AP2A2 Antibody Cat. No.: 63-513 AP2A2 Antibody Formalin-fixed and paraffin-embedded human Flow cytometric analysis of HepG2 cells using AP2A2 hepatocarcinoma with AP2A2 Antibody , which was Antibody (bottom histogram) compared to a negative peroxidase-conjugated to the secondary antibody, control cell (top histogram). FITC-conjugated goat-anti- followed by DAB staining. rabbit secondary antibodies were used for the analysis. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human This AP2A2 antibody is generated from rabbits immunized with a KLH conjugated IMMUNOGEN: synthetic peptide between 610-637 amino acids from the Central region of human AP2A2. TESTED APPLICATIONS: Flow, IHC-P, WB For WB starting dilution is: 1:1000 APPLICATIONS: For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 September 25, 2021 1 https://www.prosci-inc.com/ap2a2-antibody-63-513.html PREDICTED MOLECULAR 104 kDa WEIGHT: Properties This antibody is purified through a protein A column, followed by peptide affinity PURIFICATION: purification. CLONALITY: Polyclonal ISOTYPE: Rabbit Ig CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide. CONCENTRATION: batch dependent Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Additional Info OFFICIAL SYMBOL: AP2A2 AP-2 complex subunit alpha-2, 100 kDa coated vesicle protein C, Adaptor protein complex AP-2 subunit alpha-2, Adaptor-related protein complex 2 subunit alpha-2, Alpha-adaptin C, Alpha2-adaptin, Clathrin assembly protein complex 2 alpha-C large chain, Huntingtin ALTERNATE NAMES: yeast partner J, Huntingtin-interacting protein 9, HIP-9, Huntingtin-interacting protein J, Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit, AP2A2, ADTAB, CLAPA2, HIP9, HYPJ, KIAA0899 ACCESSION NO.: O94973 GENE ID: 161 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the BACKGROUND: cytoplasmic tails of membrane proteins, leading to their selection and concentration. Alpha adaptin is a subunit of the plasma membrane adaptor. It binds polyphosphoinositides. REFERENCES: 1) Scorilas,A., et.al., Gene 289 (1-2), 191-199 (2002) 2) Benmerah,A., et.al., J. Biol. Chem. 271 (20), 12111-12116 (1996) September 25, 2021 2 https://www.prosci-inc.com/ap2a2-antibody-63-513.html ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 25, 2021 3 https://www.prosci-inc.com/ap2a2-antibody-63-513.html.