An International Journal Nature and Science ISSN 1545-0740 Volume 8 - Number 8 (Cumulated No. 41), August 1, 2010 Marsland Press P.O. Box 21126, Lansing, Michigan 48909, USA 525 Rockaway PKWY, #B44, Brooklyn, New York, 11212, USA http://www.sciencepub.net http://www.sciencepub.org [email protected] [email protected] 347-321-7172 http://www.sciencepub.net/nature [email protected] Nature and Science ISSN 1545-0740 Nature and Science ISSN: 1545-0740 The Nature and Science is an international journal with a purpose to enhance our natural and scientific knowledge dissemination in the world under the free publication principle. Papers submitted could be reviews, objective descriptions, research reports, opinions/debates, news, letters, and other types of writings that are nature and science related. All manuscripts submitted will be peer reviewed and the valuable papers will be considered for the publication after the peer review. 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References should include all the authors’ last names and initials, title, journal, year, volume, issue, and pages etc. ISSN: 1545-0740 ã 2003-2011 Marsland Press http://www.sciencepub.net [email protected] Nature and Science ISSN: 1545-0740 Volume 8 - Number 7 (Cumulated No. 40), July 1, 2010 Cover Page, Introduction, Contents, Call for Papers, ns0807 Welcome to send your manuscript(s) to: [email protected]. CONTENTS No. Titles / Authors Full Text The effects of Agnucaston and Metformin on the chromosomes of pregnant Full Text females and their embryos Hanaa M.Roshdy Cell Biology Department, National Research Centre, Giza, Cairo Abstract: Background:Agnucaston (chaste berry fruits) and Metformin are the most common medications in the treatment of polycystic ovary syndrome (PCOS) and hormonal imbalance. The safe use of Agnucaston and Metformin in the virgin females and in the pregnant females and their embryos has not been adequately studied. Aim of the study is to evaluate the cytogenetic effects of Agnucaston and Metformin before and during pregnancy. Materials and Methods: The sample of this study was female mice (Virgin and pregnant) divided 1 into two groups control (did not administer any medication) and treated group (administered Agnucaston and Metformin orally) by doses of 0.3 and 1.3 mg/kg/day respectively for (15) consecutive days. After one day from the last treatment the females were sacrificed and cytogenetic analysis were conducted. Results: females treated with Agnucaston(Virgin and pregnant ) showed increase in frequencies of chromosomal aberrations significantly and also in their embryos but these increases were highly significant in pregnant females than virgin While, the females treated with Metformin there was a slight significant increase in the frequencies of the chromosomal aberrations in the pregnant females and embryos but there was no significant increase in the virgin females treated with Metformin before pregnancy. Conclusion: Our results indicate that Agnucaston has a mutagenic effects on the females (Virgin and pregnant) and on the embryos while Metformin has a slight mutagenic effects on the pregnant females and their embryos but does not have a mutagenic effects on the virgin females. [Nature and Science 2010;8(7):1-7]. (ISSN: 1545-0740). Keywords: (polycystic ovary syndrome), Agnucaston, Metformin, chromosomal aberrations, mice, embryos Effects of IBA on rooting performance of Citrus auriantifolia Swingle Full Text (Kagzi-lime) in different growing conditions Bani Bhushan Bhatt1*, Yogendra Kumar Tomar2 2 1. Department of Horticulture, GBP UA&T, College of Forestry and Hill Agriculture, Hill campus Ranichauri, Tehri Garhwal. 249 199, India 2. Department of Horticulture, HNB Garhwal University, Srinagar Garhwal, Uttarakhand 246 174, India [email protected] Abstract: Considering the unavailability of information of the effect of rooting hormones in combination with modified growing conditions on the rooting characteristics of Citrus auriantifolia Swingle cuttings under valley conditions of Garhwal Himalaya, the experiment was undertaken at the HRC, Garhwal University Srinagar, Uttarakhand, India. The effect of different concentration of Indolebutyric acid (IBA) and different growing conditions have been examined for stimulatory effects adventitious root formation in stem cutting of Kagzi-lime. Properly prepared cuttings of about 22-24 cm length in the month of June were treated with different concentrations of IBA viz., 500, 1000, 1500 ppm for 5 second by concentrated solution dip method and planted in 3 different conditions namely open area, under partial shade and under low cost polyhouse. The cuttings treated with IBA 500 ppm, performed the best in all aspects, as root formation, length of root, thickening of root and leaf sprouting in shoot, whereas, the open area growing condition was found effective in increasing the success rate of the cuttings. All the rooting parameters performance was recorded highest under polyhouse condition. Overall treatment C1M3 (IBA 500ppm and polyhouse) combination was found best in all parameters taken. [Nature and Science 2010;8(7):8-11]. (ISSN: 1545-0740). Keywords: Kagzi lime, cuttings, IBA, rooting, low-cost poly house, Garhwal Himalaya Anthropometric Measurements and Appetite Related Hormones Full Text