SM P 2019 Strasbourg, September 16-19 2019 Société Française de Société Française Spectrométrie de Masse d’Electrophorèse et d’Analyse (SFSM) Protéomique (SFEAP) www.sfsm.fr www.sfeap.fr https://smap2019.sciencesconf.org/ 1 Table des matières Planning .............................................................................................................................................. 14 PMC Plans ........................................................................................................................................... 19 Sponsors ............................................................................................................................................. 21 Plenary lectures ................................................................................................................................ 26 Biomarker discovery and validation – from shotgun proteomics to targeted methods [PL1] ..... 27 Mass spectrometry and peptide analysis: our contribution to a long lasting story [PL2] ............ 30 Machine learning methods for the interpretation of label-free proteomics data [PL3] ............... 31 Mass spectrometric epitope mapping [PL4] ................................................................................. 32 Proteomes in 3D [PL5] ................................................................................................................... 34 Mass spectrometry approaches to dynamic protein structure: from disorder to membrane pores [PL6] ............................................................................................................................................... 35 Direct-Injection Mass Spectrometry (DIMS) for the analysis of aroma compounds [PL7] ........... 36 Hair reveals what people conceal: biometric traits from the chemical analysis of human hair [PL8] ............................................................................................................................................... 37 From analytical proteomics to biology or from biology to analytical proteomics ? [PL9] ............ 38 Oral communications ....................................................................................................................... 39 Parallel Session 1 : Ion Mobility ......................................................................................................... 40 Structure determination of large isomeric oligosaccharides of natural origin through multi-pass and multi-stage cyclic traveling wave ion mobility mass spectrometry [KL1] .............................. 41 Temperature-controlled IMS-IMS measurements for conformational thermodynamics [OC1] .. 43 Circular dichroism mass spectrometry of nucleic acids ions [OC2] ............................................... 45 Intact- and middle-level Collision Induced Unfolding experiments to decipher gas-phase unfolding mechanism of hybrid and canonical mAbs [OC3] ......................................................... 47 Parallel Session 2 : Clinical Mass Spectrometry ................................................................................ 49 Towards in-vivo molecular diagnostics of esogastric cancer with SpiderMass real-time, mini invasive analysis [KL2] ................................................................................................................... 50 Multiplexed detection and quantification of the protein effectors of antibiotic resistance in clinical strains of Pseudomonas aeruginosa and Enterobacteriaceae by Scout-MRM – Application to positive blood cultures [OC4] .................................................................................................... 52 Quantitative determination of IGF-I in blood using DBS and VAMS after solid phase extraction microelution and analysis by LC-HRMS for doping control purposes [OC5] ................................. 54 De novo sequencing of patient-derived monoclonal antibody light chains by top-down and bottom-up proteomics [OC6] ........................................................................................................ 56 Parallel Session 3 : Bio-Chemo-Informatics and Statistics ...................................................... 58 Striking the current metaproteomics dogma for deeper characterization of microbiota [KL3] ... 59 Proteomics and metabolomics data integration for deep phenotyping [OC7] ............................. 61 2 MetGem software for the generation of molecular networks based on the t-SNE algorithm [OC8] ....................................................................................................................................................... 64 Replacing target-decoy competition to stabilize the FDR control of peptide identification in MS- based discovery proteomics [OC9] ................................................................................................ 66 Parallel Session 4 : Instrumentation ........................................................................................... 68 Andromede: MeV-Nanoparticle-SIMS for surface analysis [KL4] .................................................. 69 Benefits of Py-GCxGC/MS for the analysis of complex samples from cultural heritage [OC10] ... 71 BLI-MS: coupling BioLayer Interferometry with Mass Spectrometry [OC11] ............................... 73 Implementation and evaluation of a homemadenanoflow interface for capillary electrophoresis- mass spectrometry coupling [OC12] ............................................................................................. 75 Parallel Session 5 : Proteomic Sciences 1 ................................................................................... 77 Proteomics for hunting molecular targets to combat biofilms and proteomics again for deciphering associated molecular mechanisms [KL5] ................................................................... 78 Critical role of a phosphorylation in sheath assembly and function of an atypical type VI secretion system [OC13] ............................................................................................................... 79 Functional proteomics of glioblastoma: investigation of tumor initiating cell secretome [OC14] 81 Dynamic analysis of the VAV1 interactome in primary T cells reveals a crosstalk between the TCR and CD226 [OC15] ......................................................................................................................... 83 Parallel Session 6 : Structural Characterization ........................................................................ 85 From molecular recognition to cellular function: Pex5p receptor phosphorylation controls peroxisomal matrix protein import [KL6] ...................................................................................... 86 Negatively charged liganded gold clusters: where is the charge? Insight from IMS-MS [OC16] .. 88 Distribution study of paracetamol and its metabolites in rat whole body after on-tissue chemical derivatization by MALDI Imaging Mass Spectrometry [OC17] ...................................................... 90 Glycoproteomic study of Saccharomyces cerevisiae yeast cell wall mannoproteins [OC18] ....... 92 Parallel Session 7 : Structural Biology ........................................................................................ 94 Study of the largest and most heterogeneous macromolecular complex by HDX-MS, bringing new important mechanistic insights in proteasome regulation [KL7] .......................................... 95 Structure of Aβ42 pore-forming oligomers [OC19] ....................................................................... 97 Benzophenone photoreactivity in a lipid bilayer to probe cell-penetrating peptides/lipid membrane interactions by affinity photocrosslinking coupled to mass spectrometry [OC20] .. 100 An innovative in vivo cross-linking mass spectrometry workflow for proteome-wide studies [OC21] .......................................................................................................................................... 102 Parallel Session 8 : Geochemistry / Environment / Polymers ............................................... 104 Laser Desorption Ionisation coupled to FTICR for analysis of whole soils [KL8] ......................... 105 Robust proteomics workflow for the high throughput identification and classification by MALDI FT-ICR MS of paleontological bones indiscernible by their anatomy [OC22] ............................. 107 Non-targeted analysis applied to archaeological samples for a fast composition overview [OC23] ..................................................................................................................................................... 109 3 Analysis of bitumen by high performance thin layer chromatography coupled to laser desorption ionization Fourier transform ion cyclotron resonance mass spectrometry [OC24] .................... 111 Parallel Session 9 : Proteomic Sciences 2 ................................................................................. 113 Quantitative determination of Staphylococcus aureus enterotoxins in complex food matrices by a multiplex immunocapture mass spectrometry [KL9] ............................................................... 114 Quantifying the cellular redoxome : effect of MnSOD mimic, a potential metallodrug against Inflammatory Bowel Diseases [OC25] ......................................................................................... 116 Development of quantitative targeted proteomics