M. THEVIS H. GEYER U. MARECK (EDITORS) RECENT ADVANCES IN DOPING ANALYSIS (27) Proceedings of the Manfred Donike Workshop 37th Cologne Workshop on Dope Analysis 17th to 22nd February 2019 SPORTVERLAG Strauß - Hellenthal 2019 Bibliografische Information Der Deutschen Nationalbibliothek Die Deutsche Nationalbibliothek verzeichnet diese Publikation in der Deutschen Nationalbibliografie; detaillierte bibliografische Daten sind im Internet über <http://dnb.d‐nb.de> abrufbar. Thevis, Mario; Geyer, Hans; Mareck, Ute (Eds.) Recent Advances in Doping Analysis (27). Proceedings of the Manfred Donike Workshop, 37th Cologne Workshop on Dope Analysis, 17th to 22nd February 2019 / [MDI e.V.] – 2019 Sportverlag Strauß. ISBN 978‐3‐86884‐045‐2 ©SPORTVERLAG Strauß Neuhaus 12 – 53940 Hellenthal Tel. +49 (0)2448/2470040 – Fax +49 (0)2448/9195610 E‐mail: info@sportverlag‐strauss.de www.sportverlag‐strauss.de Satz: Autorensatz RECENT ADVANCES IN DOPING ANALYSIS (27) 2 ISBN 978-3-86884-045-2 MANFRED DONIKE WORKSHOP 2019 TABLE OF CONTENTS LECTURES PAGE Perspective Article: Biasini GM, Botrè F, de la Torre X, Donati F: Electrical brain stimulation: the rise of a new doping method? ................................................ 11‐16 POSTER PRESENTATIONS Piper T, Thevis M: Do urinary concentrations of dimethyl sulfoxide and its metabolite dimethyl sulfone indicate topical administrations of doping agents? ..................................................................... 17‐20 Muñoz G , Baltazar‐Martins JG , Plata MDM , Muñoz‐Guerra J , Carreras D , del Coso J: Prevalence of use of tramadol in conjunction with other non‐ prohibited stimulants ............. 21‐24 Martin L, Martin J, Hoang O, Collot D, Audran M, Marchand A: Detection of rEPOs in plasma from ABP blood samples: impact of freeze‐thaw cycle and hemolysis ................................................................................................................................ 25‐29 Romberg S, Kempkes R, Thevis M: Athlete Biological Passport in Practice: Computer Assisted Evaluation of Sysmex Measurements at the Analysis Site .............................................................................................. 30‐34 Fußhöller G, Geyer H, Schänzer W, Thevis M: Bacterial activities in urine samples: Formation of metandienone and 6β‐hydroxy‐ metandienone from the internal standard methyltestosterone – a case study ........................ 35‐38 Rodriguez Fernandez A, Martinez Brito D, Correa Vidal T, Lopez Castillo D, Montes de Oca Porto R: Evaluation of the steroid profile in Latin‐American population of male football players ......... 39‐44 Iannone M, di Murro V, de la Torre X, Botrè F: Effects of non‐banned and non‐monitored substances ‐ synthetic isoflavones, resveratrol and polydatin ‐ on the parameters of the urinary steroid profile ........................... 45‐49 Comunità F, Botrè F, Grippo D, Montesi G, de la Torre X, Mazzarino M: Azole antifungals and their effect on the urinary steroid profile: a dose response relationship study .......................................................................................................................... 50‐53 RECENT ADVANCES IN DOPING ANALYSIS (27) 3 ISBN 978-3-86884-045-2 PAGE Oliveira FA, Oliveira FAG, Labanca BDJ, Padilha MC, Pereira HMG: Report of four months experiencing the 2018 Technical Document for endogenous anabolic androgenic steroids in LBCD ......................................................................................... 54‐57 Masquelier M, Andersson A, Ericsson M: Confirmation of oxandrolone metabolites or the story of a false ”false positive” ................... 58‐61 Fußhöller G, Guddat S, Schänzer W, Geyer H, Thevis M: Testolactone – Derivatisation Artifact due to the Incorporation of an Ethylmercapto Group ............................................................................................................................................ 62‐65 Shiomura S, Kageyama S, Okano M: Urinary steroid profiles and carbon isotope ratios after administration of testosterone gel in Japanese subjects ............................................................................................................... 66‐70 Jardines D, Curcio D, Colamonici C, Botrè F, de la Torre X: Inter‐individual behavior of the endogenous reference compounds ........................................ 71‐74 Ericsson M, Thörngren J, Hopcraft O: A new sample preparation procedure for GC‐C‐IRMS analysis using mixed mode SPE and MS/MS detection for HPLC‐fractionation ............................................................................ 75‐80 Ndlovu F , Van der Merwe M , Goebel C , Grainger J: Development and validation of a method for the detection of small peptides in doping control .............................................................................................................................. 81‐86 Radu M , Berghes B , Toboc A: Development and validation of a LC‐HRMS method for the detection of small peptides in human urine .............................................................................................................. 87‐90 Pettersson‐Bohlin K, von Walden J, Ericsson M, Thörngren J: Confirmation of derivatized methylhexanamine and its isomers using Q‐exactive ................. 91‐93 Olgaz Bingöl S, Onol S, Aytekin S, Basaran AA: Differentiation of Modafinil and Dimenhydrinate by UPLC‐ESI‐MS/MS in Doping Analysis ......................................................................................................................................... 94‐97 Krug O , Thomas A , Walpurgis K , Piper T , Geyer H , Thevis M: Black market products suspected to contain doping relevant ingredients ‐ annual report for 2018 ............................................................................................................................ 98‐102 PRESENTATIONS ABSTRACTS ‐ LECTURES Garzinsky AM , Walpurgis K , Krug O , Thevis M: Does saliva contribute to exhaled breath samples collected by means of an electret membrane? ...................................................................................................................................... 103 RECENT ADVANCES IN DOPING ANALYSIS (27) 4 ISBN 978-3-86884-045-2 PAGE Van Wagoner R, Miller G, Husk J, Bruno B, Eichner D: Investigating oral fluid and exhaled breath as alternative matrices for anti‐doping testing: a collaborative study with minor league baseball and the partnership for clean competition ............................................................................................................................. 104 Pereira HM, Sardela V, Matos R, Prado E, Gomes G, Lima de Sá C, Anselmo C, Nunes I, Ramalho G, Padilha M, Vanini G, Oliveira MA, Oliveira A, Amaral L, Canuto C, Siqueira D, Ambrósio J, de Aquino Neto FR: Zebrafish water tank model: scale down, study pipeline and reference collections .................... 105 Coll Camenforte S , Matabosch Gerones X , Llorente Onaindia J , Pérez Mañá C , Monfort Mercader N, Monfort Faure J , Ventura Alemany R: Elimination profile of triamcinolone hexacetonide and its metabolites in human urine and plasma after a single intra‐articular administration ................................................................ 106 Reichel C, Fröschl H, Gmeiner G: Establishing doping‐related reference distributions for cobalt in human urine .................... 107‐108 Lange T , Walpurgis K , Thomas A , Geyer H , Thevis M: Development of two complementary LC‐HRMS methods for analyzing Sotatercept in dried blood spots for doping controls ............................................................................................. 109 Thomas A, Thevis M: Identification of metabolites of peptide‐derived drugs using skin tissue microsomes and Isotope‐labeled Reporter Ion Detection strategy ................................................................... 110 Piper T, Fusshöller G, Schänzer W, Thevis M: Investigations into the in vivo metabolism of Methylstenbolone .......................................... 111‐112 Cavalcanti G, Gujar A, Pereira H, Padilha M: Advanced Electron Ionization source (AEI source) : Improving the reliability of confirmation methods in sports drug testing ................................................................................. 113 Polet M, Van Gansbeke W, Albertsdóttir AD, Coppieters G, Deventer K, Van Eenoo P: Analysis of non‐hydrolyzed sulfated steroids by gas chromatography mass spectrometry ......... 114 Martinez‐Brito D, de la Torre X, Botrè F: 7‐Keto‐DHEA: metabolite or precursor? Potential pitfalls in interpreting the analytical results and WADA list ...................................................................................................................... 115 Mazzarino M, Martellone L, Comunità F, de la Torre X, Botrè F: Evaluation of the efficacy of the protocols used in the anti‐doping tests to reveal the presence of 5α‐reductase inhibitors ............................................................................................... 116 Alechaga E, Esquivel A, Monfort N, Ventura R: Potential of endogenous steroid sulfate metabolites as markers of intramuscular administration of testosterone in Caucasian and Asian population .............................................. 117 Miller G, Bruno B, Eichner D, Thevis M: Using pigs as an in vivo metabolic model for performance‐enhancing drugs ............................... 118 RECENT ADVANCES IN DOPING ANALYSIS (27) 5 ISBN 978-3-86884-045-2 PAGE Ponzetto F, Nicoli R, Kuuranne T, Saugy M, Boccard