M L E I M G B A R N A N D E T & A R G R NPFF1 Neuropeptide FF Receptor, E E T C S Subtype 1 (Human, Recombinant) Y E S T P L I G A N D & R E C E P T O R U P D A T E E RBHNF1M T M O S R Background Packaging and Storage The human NPFF1 receptor was cloned in 2000 • Membranes are suspended in 50 mM Tris-HCl, U from a human spinal cord cDNA library (Bonini et o P pH 7.4 @ 27 C, 1mM MgCl2, 60mM NaCl, 0.5% al.). NPFF is the endogenous agonist acting on this BSA. D receptor as well as on the NPFF2 receptor. This central nervous system octapeptide (NPFF) is • Available in 400 UA vials (enough for 400 assay A known to interfere in pain modulation, insulin units) T release, food intake, memory, blood pressure and E o opiate tolerance. The NPFF C-terminal (arg-phe- • Store at –70 C amide) is the sequence recognized by the NPFF1 receptor. NPFF seems to inhibit adenylyl cyclase through the Gi protein coupled to NPFF1 receptor (Hinuma et al., 2000). NPFF1 receptor has a high Availability sequence homology to the NPFF2 receptor (amino acid sequence identity of 59% (Bonini et al.)) and Please contact your local PerkinElmer sales office to orexin, NPY and cholecystokinin families. for availability. www.perkinelmer.com/lasoffices Features Related Products 125 Cat. No. Size • B for [ I] NPFF (PerkinElmer NEX381) : 2.7 max 125 pmol/mg protein NEX381 [ I] NPFF 125 • Kd for [ I] NPFF binding: 0.084 nM • Protein Concentration: 0.8 mg/ml References: • Host cell: CHO-DHFR(-) Bonini, J.A. et al., J. Biol. Chem. 275: 39324-39331 (2000) • GenBank Accession Number: NM_022146 Hinuma, S. et al., Nat. Cell Biol. 2: 703-708 (2000) www.perkinelmer.com PerkinElmer Life and Analytical Sciences 710 Bridgeport Avenue Shelton, CT 06484-4794 USA Phone: (800) 762-4000 or (+1) 203-925-4602 www.perkinelmer.com For a complete listing of our global offices, visit www.perkinelmer.com/lasoffices ©2004 PerkinElmer, Inc. All rights reserved. The PerkinElmer logo and design are registered trademarks of PerkinElmer, Inc. All other trademarks not owned by PerkinElmer, Inc. or its subsidiaries that are depicted herein are the property of their respective owners. PerkinElmer reserves the right to change this document at any time and disclaims liability for editorial, pictorial or typographical errors. 007130_01 Printed in USA .