JOURNAL OF VIROLOGY Volume 52 January 1985 No. 1 ANIMAL VIRUSES Differential Stability of Host mRNAs in Friend Erythroleukemia Cells Infected with Herpes Simplex Virus Type 1. Barbara A. Mayman and Yutaka Nishioka ............................................................ 1-6 Myristic Acid, a Rare Fatty Acid, Is the Lipid Attached to the Transforming Pro- tein of Rous Sarcoma Virus and Its Cellular Homolog. Janice E. Buss and Bartholomew M. Sefton ............. ................................. 7-12 Genome Organization of Herpesvirus Aotus Type 2. Pawel G. Fuchs, Rudiger Ruger, Herbert Pfister, and Bernhard Fleckenstein ...... ................ 13-18 Isolation and Structural Mapping of a Human c-src Gene Homologous to the Transforming Gene (v-src) of Rous Sarcoma Virus. Carol P. Gibbs, Akio Tanaka, Stephen K. Anderson, Janet Radul, Joseph Baar, Anthony Ridgway, Hsing-Jien Kung, and Donald J. Fujita ........................ 19-24 Molecular Basis of Host Range Variation in Avian Retroviruses. Andrew J. Dorner, Jonathan P. Stoye, and John M. Coffin ......................... 32-39 Assignment of the Temperature-Sensitive Lesion in the Replication Mutant Al of Vesicular Stomatitis Virus to the N Gene. M. David Marks, Jennifer Kennedy-Morrow, and Judith A. Lesnaw .............................. 44-51 Mapping of the Structural Gene of Pseudorabies Virus Glycoprotein A and Identi- fication of Two Non-Glycosylated Precursor Polypeptides. Thomas C. Mettenleiter, Noemi Lukacs, and Hanns-Joachim Rziha ..... ............ 52-57 Preliminary Characterization of an Epitope Involved in Neutralization and Cell Attachment That Is Located on the Major Bovine Rotavirus Glycoprotein. Marta Sabara, James E. Gilchrist, G. R. Hudson, and L. A. Babiuk ...... 58-66 Use of a Bacterial Expression Vector to Map the Varicella-Zoster Virus Major Glycoprotein Gene, gC. Ronald W. Ellis, Paul Malcolm Keller, Robert S. Lowe, and Robert A. Zivin ........................................... 81-88 Presence of Markers for Virulence in the Unique Short Region or Repeat Region or Both of Pseudorabies Hybrid Viruses. Anton Berns, Ans van den Ouweland, Wim Quint, Jan van Oirschot, and Arno Gielkens ..... ....... 89-93 Comparison of Endogenous Murine Leukemia Virus Proviral Organization and RNA Expression in 3-Methylcholanthrene-Induced and Spontaneous Thymic Lymphomas in RF and AKR Mice. Jeffrey Chinsky, Maureen M. Goodenow, Marian Jackson, Frank Lilly, Leslie Leinwand, and Geoffrey Childs .............................................................. 94-99 Envelope and Long Terminal Repeat Sequences of a Cloned Infectious NZB Xeno- tropic Murine Leukemia Virus. Raymond R. O'Neill, Charles E. Buckler, Theodore S. Theodore, Malcolm A. Martin, and Roy Repaske ..... ...... 100-106 Characterization of a Major Protein with a Molecular Weight of 160,000 Associat- ed with the Viral Capsid of Epstein-Barr Virus. Ben Vroman, Janos Luka, Moses Rodriguez, and Gary R. Pearson ................................ 107-113 Continued on following page Continued from preceding page Middle Tumor Antigen of Polyomavirus Transformation-Defective Mutant NG59 Is Associated with pp6oCsrc. Joseph B. Bolen and Mark A. Israel ........ 114-119 Genetic and Biochemical Analysis of Transformation-Competent, Replication-De- fective Simian Virus 40 Large T Antigen Mutants. M. Michele Manos and Yakov Gluzman ..................................................... 120-127 Epitopes of Herpes Simplex Virus Type 1 Glycoprotein gC Are Clustered in Two Distinct Antigenic Sites. Steven D. Marlin, Thomas C. Holland, Myron Levine, and Joseph C. Glorioso ....................................... 128-136 Evidence for at Least Two Dominant Neutralization Antigens on Human Rhinovi- rus 14. Barbara Sherry and Roland Rueckert .......................... 137-143 Accumulation of Herpes Simplex Virus Type 1 RNAs of Different Kinetic Classes in the Cytoplasm of Infected Cells. Elizabeth Harris-Hamilton and Steven L. Bachenheimer .................................................... 144-151 Construction of Recombinants Between Molecular Clones of Murine Retrovirus MCF 247 and Akv: Determinant of an In Vitro Host Range Property That Maps in the Long Terminal Repeat. Christie A. Holland, John Wozney, Pamela A. Chatis, Nancy Hopkins, and Janet W. Hartley ................ 152-157 At Least Four Viral Genes Contribute to the Leukemogenicity of Murine Retro- virus MCF 247 in AKR Mice. Christie A. Holland, Janet W. Hartley, Wallace P. Rowe, and Nancy Hopkins ................................. 158-165 Demonstration of Three Major Species of Pseudorabies Virus Glycoproteins and Identification of a Disulfide-Linked Glycoprotein Complex. Noemi Lukacs, Heinz-Jurgen Thiel, Thomas C. Mettenleiter, and Hanns-Joachim Rziha .. 166-173 A Soluble Transcription System Derived from Purified Vaccinia Virions. Fred Golini and Joseph R. Kates ........................................... 205-213 A Monoclonal Antibody Specific for a 52,000-Molecular-Weight Human T-Cell Leukemia Virus-Associated Glycoprotein Expressed by Infected Cells. Marjorie Robert-Guroff and Emily Shepard ............................. 214-220 In Vitro Transcription of Herpes Simplex Virus Genes: Identification of a New Initiation Site and Second Intervening Sequence in the Immediate-Early RNA-5 Gene. Kristine L. Engstrom Fontichiaro, Thomas W. Beck, and Robert L. Millette ................. .................................. 235-242 Anatomy of the Herpes Simplex Virus 1 Strain F Glycoprotein B Gene: Primary Sequence and Predicted Protein Structure of the Wild Type and of Monoclo- nal Antibody-Resistant Mutants. Philip E. Pellett, Konstantin G. Kousoulas, Lenore Pereira, and Bernard Roizman ...................... 243-253 Constitutive Expression of Epstein-Barr Virus-Encoded RNAs and Nuclear Anti- gen During Latency and After Induction of Epstein-Barr Virus Replication. Ronald Weigel, Duncan K. Fischer, Lee Heston, and George Miller ...... 254-259 Analysis of Viral and Somatic Activations of the cHa-ras Gene. Clifford J. Tabin andand~~~~RobertRoetAA. Weinberg.260-265eneg...................................................... 26-5 Purification of a Soluble Template-Dependent Rhinovirus RNA Polymerase and Its Dependence on a Host Cell Protein for Viral RNA Synthesis. Casey D. Morrow, John Lubinski, Janet Hocko, Gwendolyn F. Gibbons, and Asim Dasgupta ........................................................... 266-272 Analysis of Herpesvirus Saimiri Structural Proteins with Monoclonal Antibodies. John E. Dahlberg, Eric Zintz, and Dharam V. Ablashi ................... 279-286 Integration of Spleen Focus-Forming Virus Proviruses in Friend Tumor Cells. Francoise Moreau-Gachelin, Jacqueline Robert-Lezenes, Franqoise Wendling, Armand Tavitian, and Pierre Tambourin ...... ............... 292-295 Continued on following page Continued from preceding page Epstein-Barr Virus Transformation of Human B Lymphocytes Despite Inhibition of Viral Polymerase. John W. Sixbey and Joseph S. Pagano ............. 299-301 Naturally Occurring BK Virus Variants (JL and Dik) with Deletions in the Puta- tive Early Enhancer-Promoter Sequences. Jan ter Schegget, Cees J. A. Sol, Elly Wouters Baan, Jan van der Noordaa, and Hans van Ormondt ........ 302-305 Differences in Regulatory Sequences of Naturally Occurring JC Virus Variants. J. D. Martin, D. M. King, J. M. Slauch, and R. J. Frisque ............... 306-311 Transcriptional Mapping of the Vaccinia Virus DNA Polymerase Gene. Elaine V. Jones and Bernard Moss .......... ................................ 312-315 Mapping of a Vaccinia Host Range Sequence by Insertion into the Viral Thymi- dine Kinase Gene. fylvie Gillard, Daniele Spehner, and Robert Drillien 316-318 Biologically Active Peptides of the Vesicular Stomatitis Virus Glycoprotein. Richard Schlegel and Marta Wade ..................................... 319-323 RNase H-Mediated Release of the Retrovirus RNA Polyadenylate Tail During Re- verse Transcription. John C. Olsen and Kenneth F. Watson ............. 324-329 Generation of a Uniform 3' End RNA of Murine Leukemia Virus. Alik Honigman, Anat Bar-Shira, Hana Silberberg, and Amos Panet .... ....... 330-334 A Determinant of Polyomavirus Virulence Enhances Virus Growth in Cells of Re- nal Origin. Joseph B. Bolen, Suzanne E. Fisher, Kamal Chowdhury, Tai-Chi Shan, Jeannette E. Williams, Clyde J. Dawe, and Mark A. Israel 335-339 BACTERIAL VIRUSES Transfection of REP- Mycoplasmas with Viral Single-Stranded DNA. Todd L. Sladek and Jack Maniloff ............................................. 25-31 Assembly-Controlled Autogenous Modulation of Bacteriophage P22 Scaffolding Protein Gene Expression. Sherwood Casjens, Mark B. Adams, Carol Hall, and Jonathan King ................................................... 174-179 Bacteriophage P22 Tail Protein Gene Expression. Mark B. Adams, H. Richard Brown, and Sherwood Casjens ........................................ 180-184 Posttranscriptional Modulation of Bacteriophage P22 Scaffolding Protein Gene Expression. Sherwood Casjens and Mark B. Adams .................... 185-191 Autoregulation of the Bacteriophage P22 Scaffolding Protein Gene. Elizabeth Wyckoff and Sherwood Casjens ......... .............................. 192-197 Involvement of DNA Gyrase in Bacteriophage T7 Growth. Todd R. Steck and Karl Drlica ......................................................... 296-298 Mapping of Transcription Terminators of Bacteriophages 4X174 and G4 by Se- quence Analysis. Volker Brendel ..................................... 340-342 VIRAL PATHOGENESIS