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A'( ^ Title of the project Basic research for neuroactive materials * Duration of the November 1, 1995 ~ March 31, 1997 project Purpose of the Development of neuroacitive materials to improve neuronal defects is project one of the most important subjects in Japan that will soon become a aging society. In this project, basic research for neuroactive molecule was perfomed to develop technology for neuronal regeneration, regu ­ lation of synaptic activity and interface between artificial surface and living neurons. Summary of the A novel neurite promoting factor was discoverd and its cDNA was results cloned. Mutagenesis in vitro showed that a functional region of this factor located in a polypeptide of less than 50 aminoacids. Using neu­ ronal culture, synapse formation was found to depend on two modes of activities and long-lasting synaptic potentiation was demonstrated to depend on a macromolecules released from pre- or postsynaptic neurons. To regulate nervous activities, photoactivated caged-peptide was developed and confirmed to change in affinity to its receptor. Neurons were cultured on substrates paterned by microlithography.. Publication,patents, The results were open as 23 papers and 26 oral presentation etc. One patent was applied. Future plans The leading research for neurotechnology that had been planed based on the results of this project, started in FY 1997 to develop advanced neuronal engineering and neuroactive materials. 003 ISiSiliitWP 1C fc It a m £RB t * 7 T l' 5 #a AIJS8MI6© >fC AI# :@M56te, fibroxirosroiieice^^s t*toS, AItt g©i*£icI*. -®roflEfla6<E6grL-y hr*u. »vo. f*»©»gfflji&fr£ y h9-^©f TllD©T*@##A(%%f a, <b U o-$14£5£J!B croctii, »-«i8aT©#isaE@«T^fflflsrjMw$fi -XAStxhic, -e©m#g£ttfr"esa->X7LA©tim<!:-e;h,£Sui-ra#ig *WHtt#©SISfl:tf 2 0 1 5*PICt-;?£ffl;iaci:ttJS»]©SII T$> V , C©<k )%W;RlcMAf atA©m^%:A*A^6©^*&am • £*t tSCiH. &5fc$®i6l'!gS©l$*iT£a„ c©»£:tt5t£®!)§gu, *Bf5tlci5txT(i. 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