V OMPHALIN ISSN 1925-1858 Vol. VII, No 7 Newsletter of Oct. 29, 2016 OMPHALINA OMPHALINA, newsletter of Foray Newfoundland & Labrador, has no fixed schedule of publication, and no promise to appear again. Its primary purpose is to serve as a conduit of information to registrants of the upcoming foray and secondarily as a communications tool with members. Issues of OMPHALINA are archived in: is an amateur, volunteer-run, community, Library and Archives Canada’s Electronic Collection <http://epe. not-for-profit organization with a mission to lac-bac.gc.ca/100/201/300/omphalina/index.html>, and organize enjoyable and informative amateur Centre for Newfoundland Studies, Queen Elizabeth II Library mushroom forays in Newfoundland and (printed copy also archived) <collections.mun.ca/cdm/search/ collection/omphalina/>. Labrador and disseminate the knowledge gained. The content is neither discussed nor approved by the Board of Directors. Therefore, opinions expressed do not represent the views of the Board, Webpage: www.nlmushrooms.ca the Corporation, the partners, the sponsors, or the members. Opinions are solely those of the authors and uncredited opinions solely those of the Editor. ADDRESS Foray Newfoundland & Labrador Please address comments, complaints, contributions to the self-appointed Editor, Andrus Voitk: 21 Pond Rd. Rocky Harbour NL seened AT gmail DOT com, A0K 4N0 … who eagerly invites contributions to OMPHALINA , dealing CANADA with any aspect even remotely related to mushrooms. E-mail: info AT nlmushrooms DOT ca Authors are guaranteed instant fame—fortune to follow. Authors retain copyright to all published material, and submission indicates permission to publish, subject to the usual editorial decisions. Issues are freely available to the BOARD O F DIRECTORS CONSULTANTS public on the FNL website. Because content is protected by authors’ copyright, editors of other publications wishing to use any material, should ask first. No picture, no paper. Michael Burzynski Material should be original and should deal with the mycota PRES I DEN T of Newfoundland and Labrador. Cumulative index and Geoff Thurlow detailed Information for Authors available on our website. TRE A SURER COVER MY co L O G ica L Tina Leonard Dave Malloch Russula emetica. SE C RE ta RY NB MUSEU M André Arsenault Not. Probably R. montana. Read why inside. Jim Cornish AUD ito R No voucher, so remains unidentified. Photo from Gordon Janes Jamie Graham Voitk A: A little illustrated book of common BO nn ELL COLE JA N ES Robert MacIsaac mushrooms of Newfoundland and Labrador, written after 5-6 years of trying to match our finds to Anne Marceau LEG A L CO UNSEL Andrew May descriptions of mushrooms elsewhere. Subsequent Erin McKee BROTHERS & BURDE N years of forays have accumulated sufficient Faye Murrin material, that now we can begin to describe our own Michele Piercey-Normore species. Progress is slow, and many large lacunae Helen Spencer remain—like Russula. Therefore I am grateful to Anna Bazzicalupo and friends for opening a small Marian Wissink fenestella onto this huge genus. So far, we know only three species in the R. emetica complex in NL, none of them R. emetica; commonest is R. montana. OMPHALINA V Vol. VII, No 7 ISSN 1925-1858 OMPHALIN Oct. 29, 2016 CONTENT Editor’s comments .................................. 2 Russula emetica complex in NL Anna Bazzicalupo et al. ..................... 3 Serpentinized rock lichen survey update Michele Piercey-Normore ................... 8 Craterellus ignicolor Andrus & Maria Voitk ........................... 10 FNL on MyCoPortal Alex Kuhn et al. ................................... 12 What’s wrong with this picture? Andrus Voitk ..................................... 14 The Bishop’s sketchbook ........................ 18 NAMA 2017 Britt Bunyard ...................................... 19 Mail basket ............................................ 20 Partners ......................... inside back cover Foray 2017 Notice ................... back cover This issue and all previous issues available for download from the Foray Newfoundland & Labrador website <nlmushrooms.ca>. OMPHALINA Message from the Editor The Happy Valley-Goose Bay foray is over, as seems Clearly, the accuracy of MCP as a tool rests in our to be the peak mushroom season. The Foray Report hands: to make it the best possible, we need to be issue will be out before year end. Meanwhile, a few very meticulous about the data we keep and send autumnal blooms from the mushroom world in this in. From a scientific point of view, the stock of our somewhat fuzzy Halloween bouquet. Database Team has just risen substantially within our First of all, the final word (for now) on ourRussula organization. emetica complex. The review is too restricted to There is more to mushrooming than mushrooming. speak for the province yet, but at least we have some preliminary data to build on. Happy mushrooming! Another update is about the lichen survey on serpentinized rock, peridotite. Newfoundland has some of the biggest peridotite fields in the world, yet is one of the few places where such surveys have not been published. Very few organisms are able to live on this substrate, and those that seem to specialize are uncommon elsewhere, therefore not well known. Michele Piercey-Normore is leading a survey of each of our big peridotite fields, one a year, to gather this data. The availability of the FNL collection on MyCoPortal (MCP) is announced. MCP is a very powerful tool, accessible to researchers and the general public alike. Go and try it out, play with it a bit. To make data about all herbarial collections available and accessible online is a fantastic concept. I have used MyCoPortal to great advantage and with significant time savings on several occasions. But, as with most things idealistic, at times the reality may fall a bit short of the dream. This does not invalidate the information, but lessens its potential usefulness. We examined our own data and discovered a well- known truth: the information we get out is only as good as the information put in. FNL already altered its record keeping in response to this discovery, to provide more accurate information in the future. 2 OMPHALINA TheThe RussulaRussula emeticaemetica complexcomplex inin NLNL —preliminary—preliminary reportreport Anna Bazzicalupo, Mary Berbee, Hayden Wood, Maria Voitk, Andrus Voitk Photo: Michael Burzynski Everybody knows Russula emetica: a small (cap We compared sequences from one season’s diameter usually <6 cm), bright red Russula with a collections of Newfoundland species to known white stem about equal in length to the cap diameter, reference sequences, and present these findings here and a hot, acrid taste (title banner). Many other as a very preliminary report. small red members of the genus (after all, russula Materials and methods means red) are often included in the Russula emetica, complex by error or intent, which do not necessarily In the 2015 season an attempt was made to collect as belong to the R. emetica phylogenetic clade (Fig. 1). A many examples of the emetica complex as possible. multitude of names and descriptions of species with Collecting, harvesting and preserving gills were done similar characters have cropped up over years and by MV, identification, photography, microscopy and continents. It becomes confusing to determine which overall coordination by AV, and sequencing and of these competing names to apply to your find, which phylogenetic analysis by AB, HW and MB. Collection to deprecate as synonyms, or whether you may have was done during the FNL foray as well as immediately something totally new and undescribed. before and after. Voucher specimens were archived by AV and FNL. We extracted genomic DNA from At Foray Newfoundland & Labrador (FNL), most the specimens using Qiagen’s DNeasy Plant Mini Kit such mushrooms were identified asR . emetica, following the instructions manual. The ITS region was with R. aquosa reserved for the occasional pale- amplified using PuReTaq Ready-To-Go PCR Beads capped specimen, until a soil study1 revealed that (GE Healthcare Life Sciences) with primers ITS1F and the commonest member of the complex for which ITS4. Sequencing was performed at the Innovation DNA was recovered from our soil matched GenBank Centre at McGill University and Génome Québec and deposits identified asR. griseascens.2 The resulting The University of BC Nucleic Acid Protein Service interest in the complex resulted in a reassignment Unit. Sequences were deposited in GenBank, and of most specimens to that taxon and the tentative sequenced specimens deposited in the National identification of an additional species, R. silvestris Museum in Ottawa (DAOM)—see Table 1. (Singer) Reumaux, based on microscopic morphology.3 OMPHALINA 3 Table 1. Sequences created for this study from NL specimens and the type of R. montana. GenBank # Accession # Collection # Name Yr Location MICH12231 R. montana 1975 USA, CO KX579797 DAOM 740070 15.09.06.av03 R. montana 2015 CANADA, NL KX579799 no voucher left 15.09.06.av05 R. montana 2015 CANADA, NL KX579801 no voucher left GM15A-018 R. montana 2015 CANADA, NL KX579802 DAOM 740071 GM15A-129 R. montana 2015 CANADA, NL KX579798 DAOM 740072 15.09.06.av04 R. silvestris 2015 CANADA, NL KX579800 DAOM 740073 15.09.08.av01 R. silvestris 2015 CANADA, NL KX579806 DAOM 740074 15.10.04.av04 R. aquosa 2015 CANADA, NL KX579807 DAOM 740075 15.10.04.av05 R. aquosa 2015 CANADA, NL KX579803 DAOM 740076 GM15c-061 R. velenovskyi 2015 CANADA, NL KX579804 DAOM 740077 GM15D-089 R. sp. “Woo 50” 2015 CANADA, NL KX579805 DAOM 740078 GM15c-086 R. peckii 2015 CANADA, NL Results collection of R. emetica was found. A total of 18 specimens assumed to fit the emetica Morphologically, the first three fit the general complex were collected and processed, 8 from the description of the complex given above, and fell foray and 10 outside. Amplifiable sequences were into that clade (Fig. 1). The caps of all begin globose, successfully recovered from 11. These were identified expand to planar, developing a central depression by matching to named deposits in GenBank or UNITe.