W O 2015/120270 a L 1 3 August 2015 (13.08.2015) P O P C T

W O 2015/120270 a L 1 3 August 2015 (13.08.2015) P O P C T

(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date W O 2015/120270 A l 1 3 August 2015 (13.08.2015) P O P C T (51) International Patent Classification: Drive, Urbandale, Iowa 50323 (US). LIU, Lu; 2303 Mid- C12N 15/82 (2006.01) C07K 14/415 (2006.01) dlefield Road, Palo Alto, California 94301 (US). SCHEP- ERS, Eric; 99 Water Wheel Drive, Port Deposit, Maryland (21) International Application Number: 21904 (US). YALPANI, Nasser; 6041 North Winwood PCT/US2015/014816 Drive, Johnston, Iowa 5013 1 (US). (22) International Filing Date: (74) Agent: BAUER, S . Christopher; E . I . du Pont d e 6 February 2015 (06.02.2015) Nemours and Company, Legal Patent Records Center, (25) Filing Language: English Chestnut Run Plaza 721/2640, 974 Centre Road, P O Box 2915 Wilmington, Delaware 19805 (US). (26) Publication Language: English (81) Designated States (unless otherwise indicated, for every (30) Priority Data: kind of national protection available): AE, AG, AL, AM, 61/937,288 7 February 2014 (07.02.2014) U S AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (71) Applicants: PIONEER H I BRED INTERNATIONAL, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, INC. [US/US]; 7100 N.W. 62nd Avenue, Johnston, Iowa DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, 5013 1-1014 (US). E . I . D U PONT D E NEMOURS AND HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, COMPANY [US/US]; 1007 Market Street, Wilmington, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, Delaware 19898 (US). MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, (72) Inventors: BARRY, Jennifer; 2102 Kildee Street, Ames, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, Iowa 50014 (US). HAYES, Kevin; 15802 Brookshire TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. [Continued on nextpage] (54) Title: INSECTICIDAL PROTEINS AND METHODS FOR THEIR USE (57) Abstract: Compositions and methods for controlling Fig. 2 pests are provided. The methods involve transforming organ¬ isms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful rPtIP-65F (0.0134) for preparing plants and microorganisms that possess insect¬ Group 4 - PtIP-65Fa icidal activity. Thus, transformed bacteria, plants, plant cells, — IP plant tissues and seeds are provided. Compositions are in¬ secticidal nucleic acids and proteins o f bacterial species. The — FtIP-65Ba sequences find use in the construction o f expression vectors — PtIP-65Bb (0.0767) for subsequent transformation into organisms of interest in¬ -PtIP-65Ca (0.1191) cluding plants, a s probes for the isolation o f other homolog¬ P P ous (or partially homologous) genes. The pesticidal proteins Γ ι find use in controlling, inhibiting growth or killing Lepidop- teran, Coleopteran, Dipteran, fungal, Hemipteran and nemat¬ IPtIP-65Gd Group 2 ode pest populations and for producing compositions with W-65Ge ( insecticidal activity. PIP-65Ga (0.0086) LptiP-65Gb (0.0055) PtIP-65Hj (0,1906) — PtIP-65Hg (0.2719) Group 5 PtIP-65Hh (0.2890) RIP-65Hb (0. 1485) -PHP-65Hc(l j (0.0743) 1Group 3 o -PUP- w o 2015/120270 A i III II II 11 Hill lllll 111 III III lllll 11 Hill 11 I111 llll 11llll (84) Designated States (unless otherwise indicated, for every Published: kind of regional protection available): ARIPO (BW, GH, — with international search report (Art. 21(3)) GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, — before the expiration of the time limit for amending the TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, claims and to be republished in the event of receipt of DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, amendments (Rule 48.2(h)) LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). INSECTICIDAL PROTEINS AND METHODS FOR THEIR USE CROSS REFERENCE This application claims the benefit of US Provisional Application No. 61/937,288 filed February 07, 2014, which is incorporated herein by reference in its entirety. REFERENCE TO SEQUENCE LISTING SUBMITTED ELECTRONICALLY The official copy of the sequence listing is submitted electronically via EFS-Web as an ASCII formatted sequence listing with a file named "201 50203_5295PCT_Seq Listing" created on February 03, 2015, and having a size of 1,281 kilobytes and is filed concurrently with the specification. The sequence listing contained in this ASCII formatted document is part of the specification and is herein incorporated by reference in its entirety. FIELD This disclosure relates to the field of molecular biology. Provided are novel genes that encode pesticidal proteins. These pesticidal proteins and the nucleic acid sequences that encode them are useful in preparing pesticidal formulations and in the production of transgenic pest-resistant plants. BACKGROUND Biological control of insect pests of agricultural significance using a microbial agent, such as fungi, bacteria or another species of insect affords an environmentally friendly and commercially attractive alternative to synthetic chemical pesticides. Generally speaking, the use of biopesticides presents a lower risk of pollution and environmental hazards and biopesticides provide greater target specificity than is characteristic of traditional broad- spectrum chemical insecticides. In addition, biopesticides often cost less to produce and thus improve economic yield for a wide variety of crops. Certain species of microorganisms of the genus Bacillus are known to possess pesticidal activity against a range of insect pests including Lepidoptera, Diptera, Coleoptera, Hemiptera and others. Bacillus thuringiensis (Bt) and Bacillus popilliae are among the most successful biocontrol agents discovered to date. Insect pathogenicity has also been attributed to strains of B. larvae, B. lentimorbus, B. sphaericus and B. cereus. Microbial insecticides, particularly those obtained from Bacillus strains, have played an important role in agriculture as alternatives to chemical pest control. Crop plants have been developed with enhanced insect resistance by genetically engineering crop plants to produce pesticidal proteins from Bacillus. For example, corn and cotton plants have been genetically engineered to produce pesticidal proteins isolated from strains of Bt. These genetically engineered crops are now widely used in agriculture and have provided the farmer with an environmentally friendly alternative to traditional insect- control methods. While they have proven to be very successful commercially, these genetically engineered, insect-resistant crop plants provide resistance to only a narrow range of the economically important insect pests. In some cases, insects can develop resistance to different insecticidal compounds, which raises the need to identify alternative biological control agents for pest control. Accordingly, there remains a need for new pesticidal proteins with different ranges of insecticidal activity against insect pests, e.g., insecticidal proteins which are active against a variety of insects in the order Lepidoptera and the order Coleoptera including but not limited to insect pests that have developed resistance to existing insecticides. SUMMARY Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include nucleic acid molecules encoding sequences for pesticidal and insecticidal polypeptides, vectors comprising those nucleic acid molecules, and host cells comprising the vectors. Compositions also include the pesticidal polypeptide sequences and antibodies to those polypeptides. The nucleic acid sequences can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. The nucleotide or amino acid sequences may be synthetic sequences that have been designed for expression in an organism including, but not limited to, a microorganism or a plant. Compositions also comprise transformed bacteria, plants, plant cells, tissues and seeds. In particular, isolated or recombinant nucleic acid molecules are provided encoding Pteridophyta Insecticidal Protein-50 (PtlP-50) polypeptides including amino acid substitutions, deletions, insertions, fragments thereof, and combinations thereof and Pteridophyta Insecticidal Protein-65 (PtlP-65) polypeptides including amino acid substitutions, deletions, insertions, fragments thereof, and combinations thereof. Additionally, amino acid sequences corresponding to the PtlP-50 polypeptides and PtlP-65 polypeptides are encompassed. Provided are isolated or recombinant nucleic acid molecules of SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, SEQ ID NO: 46, SEQ ID NO: 47, SEQ ID NO: 48, SEQ ID NO: 49, SEQ ID NO: 50, SEQ ID NO: 5 1, SEQ ID NO: 52, SEQ ID NO: 53, SEQ ID NO: 54, SEQ ID NO: 55, SEQ ID NO: 56, SEQ ID NO: 57, SEQ ID NO: 58, SEQ ID NO: 59, SEQ ID NO: 60, SEQ ID NO: 6 1, SEQ ID NO: 62, SEQ ID NO: 63, SEQ ID NO: 64, SEQ ID NO: 65, SEQ ID NO: 66, SEQ ID NO: 67, SEQ ID NO: 68, SEQ ID NO: 69 or SEQ ID NO: 70 capable of encoding PtlP-50 polypeptides as well as amino acid substitutions, deletions, insertions, fragments thereof, and combinations thereof. Nucleic acid sequences that are complementary to a nucleic acid sequence of the embodiments or that hybridize to a sequence of the embodiments are also encompassed. Also provided are isolated or recombinant PtlP-50 polypeptides of SEQ ID NO: 71, SEQ ID NO: 72, SEQ ID NO: 73, SEQ ID NO: 74, SEQ ID NO: 75, SEQ ID NO: 76, SEQ ID NO: 77, SEQ ID NO: 78, SEQ ID NO: 79, SEQ ID NO: 80, SEQ ID NO: 8 1, SEQ ID NO: 82, SEQ ID NO: 83, SEQ ID NO: 84, SEQ ID NO: 85, SEQ ID NO: 86, SEQ ID NO: 87, SEQ ID NO: 88, SEQ ID NO: 89, SEQ ID NO: 90, SEQ ID NO: 9 1, SEQ ID NO: 92, SEQ ID NO: 93, SEQ ID NO: 94, SEQ ID NO: 95, SEQ ID NO: 96, SEQ ID NO: 97 or SEQ ID NO: 98 as well as amino acid substitutions, deletions, insertions, fragments thereof and combinations thereof.

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