Supplementary Materials for A simple linear relationship between resource availability and microbial community diversity Martina Dal Bello, Hyunseok Lee, Akshit Goyal, Jeff Gore. Correspondence to: [email protected] This PDF file includes: Figs. S1 to S18 Tables S1 and S2 1 Family 1.00 Aeromonadaceae Microbacteriaceae Bacillaceae Micrococcaceae Bacteriovoracaceae Microscillaceae Bdellovibrionaceae Moraxellaceae 0.75 Beijerinckiaceae Nocardiaceae Burkholderiaceae Paenibacillaceae Caulobacteraceae Planococcaceae undance b Cellulomonadaceae Pseudomonadaceae 0.50 Cellvibrionaceae Rhizobiaceae Chitinophagaceae Rhodanobacteraceae Relative a Devosiaceae Rhodobacteraceae Diplorickettsiaceae Saccharimonadaceae 0.25 Enterobacteriaceae Sphingobacteriaceae Flavobacteriaceae Sphingomonadaceae Hymenobacteraceae Spirosomaceae Hyphomicrobiaceae Streptomycetaceae Kaistiaceae Weeksellaceae 0.00 Labraceae Xanthomonadaceae Original soil sample Methylophilaceae Other Fig. S1. The soil sample used to inoculate the experimental microcosms is highly diverse and taxonomically rich. A total of 750 ASVs were detected in the soil sample using 16S rRNA amplicon sequencing at single nucleotide resolution Here the relative abundance of the 39 most prevalent families in the experimental microcosms after 7 days is shown. These families constitute less than the 20% of the relative abundance of soil taxa. 2 Single resources Cellulose 2 resources Sorbitol 4 resources Fructose 8 resources Galacturonate Xylose Starch Glucose 16 resources Glycerol Random pairing of Maltose single resources Cellobiose Sucrose Mannose Mannitol Fumarate Hydroxyproline Citrate Fig. S2. Example of random grouping of single resources in 2, 4, 8 ,16-resource combinations. A total of three random groupings were included in the experiment. The total carbon concentration is kept the same across different combinations of resources (0.1 % w/v). Also, all 15-resource combinations were included. 3 Glucose Fructose Xylose Mannose 1.00 1.00 1.00 1.00 0.75 0.75 0.75 0.75 0.50 0.50 0.50 0.50 Abundance Abundance 0.25 0.25 Abundance 0.25 0.25 0.00 0.00 0.00 0.00 Relative Abundance 246 246 246 246 Cellobiose Maltose Sucrose Citrate 1.00 1.00 1.00 1.00 0.75 0.75 0.75 0.75 0.50 0.50 0.50 0.50 Abundance Abundance 0.25 0.25 Abundance 0.25 0.25 0.00 0.00 0.00 0.00 Relative Abundance 246 246 246 246 Fumarate Galacturonate Mannitol Sorbitol 1.00 1.00 1.00 1.00 0.75 0.75 0.75 0.75 0.50 0.50 0.50 0.50 Abundance 0.25 0.25 Abundance 0.25 Abundance 0.25 Relative Abundance 0.00 0.00 0.00 0.00 246 246 246 246 Glycerol Hydroxyproline Cellulose Starch 1.00 1.00 1.00 1.00 0.75 0.75 0.75 0.75 0.50 0.50 0.50 0.50 0.25 0.25 0.25 0.25 Relative Abundance 0.00 0.00 0.00 0.00 246 246 246 246 All 16 resources All 16 resources All 16 resources All 16 resources 1.00 1.00 1.00 1.00 0.75 0.75 0.75 0.75 0.50 0.50 0.50 0.50 0.25 0.25 0.25 0.25 Relative Abundance 0.00 0.00 0.00 0.00 246 246 246 246 Dilution day Dilution day Dilution day Dilution day Aeromonadaceae Cellvibrionaceae Kaistiaceae Paenibacillaceae Sphingomonadaceae Bacillaceae Chitinophagaceae Labraceae Planococcaceae Spirosomaceae Bacteriovoracaceae Devosiaceae Methylophilaceae Pseudomonadaceae Streptomycetaceae Bdellovibrionaceae Diplorickettsiaceae Microbacteriaceae Rhizobiaceae Weeksellaceae Family Beijerinckiaceae Enterobacteriaceae Micrococcaceae Rhodanobacteraceae Xanthomonadaceae Burkholderiaceae Flavobacteriaceae Microscillaceae Rhodobacteraceae Others Caulobacteraceae Hymenobacteraceae Moraxellaceae Saccharimonadaceae Cellulomonadaceae Hyphomicrobiaceae Nocardiaceae Sphingobacteriaceae Fig. S3. The majority of communities reached equilibrium before the end of the experiment. Each panel shows the temporal trajectories of the composition of one community at the family level. The most prevalent 37 families are included. The first 16 plots depict communities grown on a single carbon source. The last four plots depict replicated communities grown on a media containing all the 16 carbon sources. 4 Fig. S4. Sequencing depth did not affect richness estimates. There is no correlation between the richness of a sample and the number of reads obtained for that sample (number of samples 227). The estimated Pearson correlation coefficient is not different from zero. 5 Pseudomonadaceae Pseudomonadaceae Pseudomonadaceae Pseudomonadaceae Pseudomonadaceae Pseudomonadaceae Pseudomonadaceae Pseudomonadaceae Pseudomonadaceae Pseudomonadaceae Pseudomonadaceae Pseudomonadaceae Pseudomonadaceae Pseudomonadaceae Pseudomonadaceae Pseudomonadaceae Pseudomonadaceae Pseudomonadaceae Pseudomonadaceae Enterobacteriaceae Pseudomonadaceae Pseudomonadaceae Pseudomonadaceae Pseudomonadaceae Pseudomonadaceae Pseudomonadaceae Xanthomonadaceae Xanthomonadaceae Xanthomonadaceae Xanthomonadaceae Xanthomonadaceae Xanthomonadaceae Xanthomonadaceae Xanthomonadaceae Xanthomonadaceae Xanthomonadaceae Xanthomonadaceae Xanthomonadaceae Xanthomonadaceae Xanthomonadaceae Rhodanobacteraceae Rhodanobacteraceae Rhodanobacteraceae Rhodanobacteraceae Rhodanobacteraceae Cellvibrionaceae Moraxellaceae Moraxellaceae Moraxellaceae Moraxellaceae Moraxellaceae Moraxellaceae Aeromonadaceae Gammaproteobacteria Enterobacteriaceae Enterobacteriaceae Enterobacteriaceae Enterobacteriaceae Enterobacteriaceae Enterobacteriaceae Enterobacteriaceae Enterobacteriaceae Enterobacteriaceae Enterobacteriaceae Burkholderiaceae Burkholderiaceae Rhodocyclaceae Methylophilaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Burkholderiaceae Bdellovibrionaceae Deltaproteobacteria Caulobacteraceae Caulobacteraceae Caulobacteraceae Caulobacteraceae Sphingomonadaceae Sphingomonadaceae Sphingomonadaceae Sphingomonadaceae Sphingomonadaceae Sphingomonadaceae Sphingomonadaceae Sphingomonadaceae Sphingomonadaceae Sphingomonadaceae Rhizobiaceae Rhizobiaceae Rhizobiaceae Rhizobiaceae Rhodobacteraceae Rhizobiaceae Rhodobacteraceae Rhizobiaceae Rhizobiaceae Rhizobiaceae Rhizobiaceae Rhizobiaceae Rhizobiaceae Rhizobiaceae Alphaproteobacteria Rhizobiaceae Rhizobiaceae Rhizobiaceae Rhizobiaceae Rhizobiaceae Rhizobiaceae Rhizobiaceae Rhizobiaceae Rhizobiaceae Rhizobiaceae Hyphomicrobiaceae Labraceae Kaistiaceae Kaistiaceae Devosiaceae Devosiaceae Devosiaceae Devosiaceae Devosiaceae Devosiaceae Xanthobacteraceae Xanthobacteraceae Beijerinckiaceae Beijerinckiaceae Beijerinckiaceae Beijerinckiaceae Beijerinckiaceae Beijerinckiaceae Streptomycetaceae Nocardiaceae Nocardiaceae Nocardiaceae Micrococcaceae Micrococcaceae Micrococcaceae Micrococcaceae Actinobacteria Demequinaceae Cellulomonadaceae Promicromonosporaceae Promicromonosporaceae Microbacteriaceae Microbacteriaceae Microbacteriaceae Microbacteriaceae Sericytochromatia Hymenobacteraceae Spirosomaceae Sphingobacteriaceae Sphingobacteriaceae Sphingobacteriaceae Sphingobacteriaceae Sphingobacteriaceae Sphingobacteriaceae Sphingobacteriaceae Sphingobacteriaceae Sphingobacteriaceae Sphingobacteriaceae Bacteroidia Chitinophagaceae Chitinophagaceae Chitinophagaceae Chitinophagaceae Chitinophagaceae Weeksellaceae Flavobacteriaceae Flavobacteriaceae Flavobacteriaceae Flavobacteriaceae Flavobacteriaceae Flavobacteriaceae Flavobacteriaceae Weeksellaceae Weeksellaceae Weeksellaceae Weeksellaceae Weeksellaceae Weeksellaceae Weeksellaceae Weeksellaceae Planococcaceae Bacillaceae Bacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Bacilli Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Paenibacillaceae Starch Citrate Xylose Sorbitol Maltose Glycerol Sucrose Glucose Mannitol Fructose Mannose Cellulose Fumarate Cellobiose Galacturonate Hydroxyproline Fig. S5. Single carbon sources support microbial assemblages spanning a wide phylogenetic diversity. The pool of ASVs found across all media supplied with single resources is phylogenetically diverse, encompassing 7 classes, indicated by colored lines on the right side of the plot (black lines indicate ASVs that could not be identified at any taxonomical level except the Domain, Bacteria). Families are indicated on the left side of the phylogenetic tree. Colored tiles indicate the media in which ASV is found (for each carbon source, there are three replicated microcosms for a total of 48 communities.) Carbon sources are ordinated in an increasing order based on the average richness they support. 6 Fig. S6. Richness of microbial communities in single resources is not explained by the molecular weight of the supplied resource.