TECHNICAL DATA Glusulase SHEET Caution: For Laboratory Use. A product for research purposes only GLUSULASE beta-GLUCURONIDASE AND beta-GLUCURONIDE SULFATASE (10 ml) Product Number: NEE154001EA Glusulase contains both sulfatase and glucuronidase from snail Helix Pomatia (1032-R) Specific Lot Data: Lot# 2493756 Glucuronidase Concentration (units/ml) 203,682.85 Sulfatase Concentration (units/ml) 9,084.46 Production date 10/26/2018 Biocide: 0.1% Kathon *the biocide no longer contains sodium azide Recommended Storage Conditions: Do not freeze. Store at 2 - 8°C upon receipt. ACTIVITY ASSAYS A. Assay for glucuronidase (modification of the method of Fishman 1) Principal and definition of the unit: A unit of glucuronidase activity is defined as that amount of enzyme which will produce one µg of phenolphthalein in one hour at 37°C from 0.1M acetate buffer at pH 4.7, with a substrate of 6.2 x 10 -4M phenolphthalein-ß-glucuronide. The phenolphthalein is produced by cleavage of the ß-glucuronide bond in the phenolphthalein ß-glucuronide moiety. The A 552 1%/1 cm for phenolphthalein in 0.06M phosphate, 0.04M acetate buffer, pH 10.9 is 940. This is equivalent to a molar absorptivity of 29,900. B. Assay for sulfatase (modification of method of Jarrige 2 which is a modification of Huggins and Roy 4 methods) Principal and definition of the unit: A unit of sulfatase activity is defined as that amount of enzyme which will produce 20 µg of p-nitrophenol in one hour at 37°C from 0.1M acetate buffer at pH 5.8 with a substrate of 5.0 x 10 -4M potassium p-nitrophenol sulfate. The p-nitrophenol is produced by cleavage of the sulfate ester bond. The A 401 1%/1 cm for p- nitrophenol in 0.1 N NaOH is 1.35 x 10. This is equivalent to a molar absorptivity of 1.9 x 10 4. GENERAL INFORMATION: GLUSULASE is a preparation of the intestinal juice of the snail Helix pomatia. It is a deep brown, slightly viscous (gel-free) liquid. It contains a mixture of enzymes including ß-glucuronidase, sulfatase, and a cellulase. The activities of these enzymes have proven particularly useful for breaking open yeast cell walls and for hydrolysis of glucuronides and sulfates such as occur in conjugated steroids. REFERENCES: 1. Fishman, W.H., "ß-Glucuronidase" in Methods in Enzymatic Analysis by H.H. Bergmeier, Academic Press, New York (1963). 2. Jarrige, P., and Henry, R. "Etude de L'Activite Glucuronidasique du suc Digestif D'Helix Pomatia et de sa Phenolsulfatase" Bull. Soc. Chim. Biol.,34, 872. (1952). 3. Huggins, C. and Smith, D.R. "P-Nitrophenyl Sulfatase as a Substrate for the Assay of Phenolsulfatase Activity", J. Biol.Chem. 170, 391. (1947). 4. Roy, A.B., "The Steriod Sulfatase of Patella Vulgata", Biochem. J., 62, 41(1956). PerkinElmer, Inc. 549 Albany Street Boston, MA 02118 USA P: (800) 762-4000 or (+1) 203-925-4602 www.perkinelmer.com/nenradiochemicals For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs Copyright ©2010, PerkinElmer, Inc. All rights reserved. PerkinElmer ® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners. .