GOOD HEALTH Microgravity Protein Crystal Growth Workshop FINAL REPORT Workshop conducted at HudsonAlpha Center for Biotechnology HUNTSVILLE, ALABAMA OCTOBER 22-23, 2015 GOOD Contents HEALTH Report Introduction Introduction …………………………………………………………………………………………… 4 About the Organizers ………………………………………………………………………………… 5 Workshop Guide Invitee/Attendee List ………………………………………………………………………………… 6 Workshop Agenda …………………………………………………………………………………… 8 Recommendations From Workshop ……………………………………………… 10 Day 1 Session Summaries Historical Review …………………………………………………………………………………… 14 Current Microgravity Lessons Learned …………………………………………………………… 15 Theoretical Prediction ……………………………………………………………………………… 17 Panel 1: Molecules of Interest ……………………………………………………………………… 18 Panel 2: State-of-the-Art Imaging and Analysis ………………………………………………… 19 Panel 3: Laboratory-Based Crystallography ……………………………………………………… 20 Panel 4: Space-Based Crystallography Capabilities …………………………………………… 22 Day 2 Session Summaries Breakout Groups Summary ………………………………………………………………………… 24 Program Funding Strategies ………………………………………………………………………… 29 STEM Education/Outreach ………………………………………………………………………… 30 Next Steps ……………………………………………………………………………………… 31 ORGANIZED BY CONDUCTED AT Various images throughout publication are courtesy of NASA. CASIS, Center for the Advancement of Science in Space, and the CASIS Center for the Advancement of Science in Space logo are trademarks of the Center for the Advancement of Science in Space in the U.S. and/or other countries. Published 07/16. MICROGRAVITY PROTEIN CRYSTAL GROWTH WORKSHOP 3 Report Introduction Microgravity has been used for more than 30 years to improve outcomes of crystal analyses through production of higher quality and larger crystals. To examine the ABOUT THE ORGANIZERS readiness and requirements of this marketplace for a more commercial approach to crystallization on the International Space Station (ISS) U.S. National Laboratory, the Center for the Advancement of Science in Space (CASIS) held a subject matter expert workshop in October 2015 with experts across the field of crystallography. About CASIS: The Center for the Advancement of The mission of this workshop was to outline the basic science Science in Space (CASIS) was selected by NASA in requirements for a long-term protein crystallization program July 2011 to maximize use of the International Space onboard the ISS National Lab, including accessibility and Station (ISS) U.S. National Laboratory through 2020. timing, flight and ground resources, education, and funding. CASIS is dedicated to supporting and accelerating This report outlines the participants and their presentations, innovations and new discoveries that will enhance the discussions, and recommendations. CASIS intends to harness health and wellbeing of people and our planet. For the information gained from the workshop and the ongoing more information, visit www.iss-casis.org. support of this group to outline an ISS National Lab program for repetitive, low-cost crystallization in microgravity. Such a program will provide a platform for discovery to users across many disciplines—commercial, other government agencies, academia, and private research. In addition, a key component of the program will be inspiring the next generation through About the ISS National Lab: In 2005, Congress experiential learning opportunities. designated the U.S. portion of the International Space Station as the nation’s newest national laboratory to maximize its use for improving life on Earth, promoting collaboration among diverse users, and advancing STEM education. This unique laboratory environment is available for use by other U.S. government agencies and by academic and private institutions, providing access to the permanent microgravity setting, vantage point in low Earth orbit, and varied environments of space. ABOUT GOOD HEALTH GOOD HEALTH is a CASIS GOOD research initiative in partnership HEALTH with NASA to improve human health by enabling biomedical discovery in space for the benefit of life on Earth. Good Health capitalizes on the unique environment of the ISS National Lab to study, in humans and model organisms, transitions from health to disease that are accelerated by microgravity, including osteoporosis, muscle wasting, and immune dysfunction. GOOD 4 HEALTH LIFE SCIENCES MICROGRAVITY PROTEIN CRYSTAL GROWTH WORKSHOP 5 Workshop Guide INVITEE/ATTENDEE LIST Tim Allen Larry DeLucas Peter Moeck Mark Uhran Teledyne Brown Engineering University of Alabama at Birmingham Portland State University Oak Ridge National Laboratory Stephen Aller Owen Garriott Timothy Mueser Liz Wilson-Kubalek University of Alabama at Birmingham Astronaut The University of Toledo The Scripps Research Institute Benjamin Apker Sridhar Gorti Mary Murphy Kenneth Shields MiTeGen NASA Marshall Space Flight Center NanoRacks CASIS, Director of Operations Diego Arias Dorit Hanein Joseph D Ng April Spinale JAMSS America, Inc. (JAI) Sanford Burnham Prebys Medical Discovery Institute The University of Alabama in Huntsville/iExpressGenes CASIS, Operations Team Jim Baird Issa Isaac Edward Pryor Debbie Wells The University of Alabama in Huntsville Molecular Dimensions Microlytic (acquired by Anatrace) CASIS, Portfolio Project Manager Eugene Boland Greg Jenkins Marc Pusey Michael Roberts Techshot, Inc. Moseley Technical Services iExpress Genes CASIS, Senior Project Scientist Gloria Borgstahl Eric Johnson Mike Read Amelia Smith University of Nebraska Medical Center Pfizer, Inc. NASA ISS National Laboratory Manager CASIS, Technical Writer Brad Carpenter Laurel Karr Joseph Reibenspies NASA SLPSRA NASA Marshall Space Flight Center Texas A&M University Walter Chazin Andrey Kovalevsky Paul Reichert Vanderbilt University Oak Ridge National Laboratory Merck Research Laboratories John Clemente Todd Link Julie A. Robinson Art Robbins University of Texas MD Anderson Cancer Center NASA ISS Program Chief Scientist Leighton Coates Donnie McCaghren Ken Savin Oak Ridge National Laboratory NASA Marshall Space Flight Center Eli Lilly and Company Dan Connor Alexander McPherson Hiroaki Tanaka University of Alabama at Birmingham’s Center University of California, Irvine Confocal Science, Inc. for Biophysical Sciences and Engineering Ashlyn Manzella Diana Tomchick Kirt Costello University of Alabama at Birmingham’s American Crystallography Association/UT Southwestern NASA ISS Program Scientist Center for Biophysical Sciences and Engineering GOOD 6 HEALTH LIFE SCIENCES MICROGRAVITY PROTEIN CRYSTAL GROWTH WORKSHOP 7 Panel 4: Space-based Crystallography Capabilities ……………………………………………… 3:15 – 4:30P Workshop Agenda » Spinale, Moderator » O’Connor (UAB), Arias/Tanaka (JAMSS), Carter (PCAM), Pusey/Gorti (MSFC), Center for the Advancement of Science in Space (CASIS) Jenkins (Granada), Boland (Techshot), Murphy (Nanoracks), Allen (TBE) Hudson Alpha Center for Biotechnology Technical Interchange Workshop ▶ Flight approved hardware – organic and inorganic molecules Implementation of a Low Cost, Robust Protein Crystallization Program aboard the International Space Station National Lab ▶ Other items not October 22-23, 2015 Day 1 Wrap-up ………………………………………………………………………………………………… 4:30 – 4:45P DAY 1, OCTOBER 22 Depart for Hotel ………………………………………………………………………………………………… 5:00P Coffee/Pastries/Fruit …………………………………………………………………………………………… 8:00A CDT DAY 2, OCTOBER 23 Welcome/Introduction – CASIS Coffee/Pastries/Fruit …………………………………………………………………………………………… 8:00A CDT » Debbie Wells/Ken Shields – Who we are and why microgravity crystallization is important. …………… 8:30 – 8:40A Summary of Day 1 Panel Points/Break-Out Prep ………………………………………………… 8:30 – 9:00A Historical Perspective » Debbie Wells » Alex McPherson/Laurel Karr …………………………………………………………………………………… 8:45 – 9:15A Break-Out Session: Crystallization to a Set of Program Science Requirements ……… 9:00 – 10:30A Current Microgravity Investigational Lessons Learned ………………………………………… 9:15 – 10:30A » IP Reps/CASIS Reps » Larry DeLucas – Double Blind Study » Paul Reichert – Merck Pharma Program ▶ Break-out teams with representatives from distributed background to narrow down the set of “requirements” for the spaceflight program » Joe Ng – Crystals for Neutron Crystallography » Ken Savin – Eli Lilly Pharma Program ▶ One CASIS person/one CASIS IP person will lead each break-out team ▶ Notes from Day 1 Sessions provided Break (Coffee/Soda) ………………………………………………………………………………………… 10:30 – 10:45A Break (Coffee/Soda) ………………………………………………………………………………………… 10:30 – 10:45A Theoretical Prediction » Jim Baird, Professor of Chemistry UAH ……………………………………………………………………… 10:45 – 11:15A Break-Out Presentations …………………………………………………………………………………… 10:45A – 12:15P » Wells/Break-Out Teams Panel 1: Molecules of Interest …………………………………………………………………………… 11:15 – 12:00P ▶ Each break-out group presents their requirement concepts » Roberts, Moderator ▶ Note-taking/grouping of ideas » Reichert (Merck), Johnson (Pfizer), Eli Lilly (Savin), Tomchick (ACA), DeLucas (UAB), Ng (UAH) ▶ What molecules or classes of molecules should be the focus of the program? Lunch (Full Meal) ……………………………………………………………………………………………… 12:15 – 1:15P ▶ Are proteins the only molecules that should be considered? Funding Strategies – Translation of Lab to National Lab ……………………………………… 1:15 – 2:15P Lunch Seminar (Box Lunches Provided) …………………………………………………………… 12:15 – 1:00P » Wells » Debbie Wells, CASIS – Mysteries of Microgravity Fluid Dynamics ▶ Commercial ▶ Academic Panel 2: State-of-the-Art Imaging and Analyses ………………………………………………… 1:00 – 2:00P ▶ Other Government Agencies »