NeoBiotechnologies, Inc. 2 Union Square Union City, CA 94587 Tel: 510-376-5603 Email: [email protected] , [email protected] Website: www.NeoBiotechnologies.com Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Mouse Monoclonal Antibody [Clone 2A4] Catalog No Format Size Price (USD) 5420-MSM3-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00 5420-MSM3-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 459.00 5420-MSM3-P1BX Purified Ab WITHOUT BSA at 1.0mg/ml 100 ug 459.00 Human Entrez Gene ID 5420 Immunogen A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of Human SwissProt O00592 human podocalyxin Human Unigene 732423 Host / Ig Isotype Mouse / IgM, kappa Human Gene Symbol PODXL Mol. Weight of Antigen 165-170kDa Human Chromosome 7q32.3 Cellular Localization Cell surface and Cytoplasmic Location Species Reactivity Human. Rabbit. Rat. Synonyms PODXL, PDXL, GCTM-2; Gp200; PCLP1; Pcx; Podocalyxin-like protein 1 Positive Control HeLa, Raji, Jurkat cells, Angiosarcoma, Breast, Prostate, Liver, Pancreatic, Kidney. Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Podocalyxin Monoclonal Antibody (2A4). Specificity & Comments Supplied As Podocalyxin is a member of the CD34 transmembrane sialomucin family. It 200ug/ml of Ab Purified from Bioreactor Concentrate. Prepared in 10mM is over-expressed on the podocyte foot projections and plays essential roles PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide in kidney development and homeostasis, blood filtration and urine formation. at 1.0mg/ml. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more Storage and Stability aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 and other kidney pathology. to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required. Known Applications & Suggested Dilutions Limitations This antibody is available for research use only and is not approved for use in diagnosis. Flow Cytometry (1-2ug/million cells) Immunofluorescence (1-2ug/ml) Warranty Western Blot (1-2ug/ml) There are no warranties, expressed or implied, which extend beyond this Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining description. Company is not liable for any personal injury or economic of formalin-fixed tissues requires heating tissue sections in 10mM Tris with loss resulting from this product. 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined. Key References 1. Kershaw DB et. al. J Biol Chem 272:15708-15714 (1997). 2. Sassetti C et. al. J Exp Med187:1965-1975. 3. Nielsen JS et. al. J Am Soc Nephrol 20:1669-1676 (2009). NBT00483.