cdfa ~ Symposium Proceedings Proceedings of the 2013 Pierce’s Disease Research Symposium - December 2013 - Compiled by: Pierce’s Disease Control Program California Department of Food and Agriculture Sacramento, CA 95814 Chief Editor: Thomas Esser, CDFA Assistant Editor: Raj Randhawa, CDFA Cover Design and Photograph: Sean Veling, CDFA Printer: California Office of State Publishing, Sacramento, California Funds for Printing Provided by: CDFA Pierce’s Disease and Glassy-winged Sharpshooter Board Cite as: Pierce’s Disease Research Progress Reports. California Department of Food and Agriculture, Sacramento, CA. December 2013. Available on the Internet at: http://www.cdfa.ca.gov/pdcp/Research.html Acknowledgements: Many thanks to the scientists and cooperators who are conducting research on Pierce’s disease and submitted reports for inclusion in this document. Note to Readers: The reports contained in this document have not been subjected to scientific peer review. To Request a Copy of This Publication, Please Contact: Pierce’s Disease Control Program California Department of Food and Agriculture 1220 N Street, Sacramento, California 95814 Telephone: 916-900-5024 Website: http://www.cdfa.ca.gov/pdcp Revision History: • 12/05/2013 -- page 73 revised TABLE OF CONTENTS Section 1: Vector Biology and Ecology REPORTS • Optimizing Electrical Penetration Graph Settings to Record Blue-green Sharpshooter X Waves for Future Studies of Grape Host Plant Resistance to Xylella fastidiosa Inoculation Elaine A. Backus .............................................................................................................................................. 3 ABSTRACTS • Suitability of Fertilized and Unfertilized Eggs of the Glassy-winged Sharpshooter for the Egg Parasitoid Gonatocerus morrilli Rodrigo Krugner............................................................................................................................................. 11 • A Method to Quantify Glassy-winged Sharpshooter Egg Maturation Mark Sisterson ................................................................................................................................................ 12 Section 2: Vector Management REPORTS • The Riverside County Glassy-winged Sharpshooter Program in the Temecula Valley Matthew Daugherty, Tracy Pinckard, and Diane Soto................................................................................... 15 • RNA-Interference and Control of the Glassy-winged Sharpshooter and Other Leafhopper Vectors of Xylella fastidiosa Bryce W. Falk ................................................................................................................................................. 19 • Development and Use of Recombinant Homalodisca coagulata Virus-1 for Controlling the Glassy-winged Sharpshooter Bryce W. Falk, Bryony Bonning, W. Allen Miller, and Drake Stenger ......................................................... 26 • The Endocrine System of the Glassy-winged Sharpshooter, a Viable Insecticide Target Shizuo G. Kamita and Bruce D. Hammock ................................................................................................... 32 - i - Section 3: Pathogen Biology and Ecology REPORTS • Identification of a New Virulence Factor Required for Pierce’s Disease and Its Utility in Development of a Biological Control Thomas J. Burr, Patricia Mowery, Luciana Cursino, and Kameka Johnson.................................................. 41 • Characterization of the Xylella fastidiosa gcvR Gene Required for Pathogenicity Hong Lin and Xiangyang Shi......................................................................................................................... 48 • Elucidating the Process of Cell-Cell Communication in Xylella fastidiosa to Achieve Pierce’s Disease Control by Pathogen Confusion Steven Lindow................................................................................................................................................ 53 • Characterization of Xylella fastidiosa Genes Required for Pathogenicity Pamela Ronald and Bruce Kirkpatrick ........................................................................................................... 63 • Characterization of Xylella fastidiosa Lipopolysaccharide and Its Role in Key Steps of the Disease Cycle in Grapevine Caroline Roper and Dario Cantu .................................................................................................................... 74 ABSTRACTS • Initiation of a Pan-Genomic Research Project for Xylella fastidiosa Jianchi Chen ................................................................................................................................................... 84 • Identification and Characterization of Xylella fastidiosa Isolated from Coffee Plants in France Bruno Legendre, Stelly Mississipi, Valérie Olivier, Emmanuelle Morel, Dominique Crouzillat, Marie-Agnès Jacques, Françoise Poliakoff, and Charles Manceau ................................................................ 85 Section 4: Pathogen and Disease Management REPORTS • Building a Next Generation Chimeric Antimicrobial Protein to Provide Rootstock- Mediated Resistance to Pierce’s Disease in Grapevines Abhaya M. Dandekar...................................................................................................................................... 89 • Chimeric Antimicrobial Protein and Polygalacturonase-Inhibiting Protein Transgenic Grapevines Field Trial Abhaya M. Dandekar...................................................................................................................................... 95 • Field Evaluation of Grape Plants Expressing Potential Protective DNA Sequences Effective Against Pierce’s Disease David Gilchrist, Abhaya Dandekar, John Labavitch, and Steven Lindow................................................... 101 - ii - • Field Evaluation of Grape Plants Expressing PR1 and UT456 Transgenic DNA Sequences for Protection Against Pierce’s Disease David Gilchrist and James Lincoln ..............................................................................................................105 • Evaluation of Pierce’s Disease Resistance in Transgenic Vitis vinifera Grapevines Expressing Either Grape Thaumatin-Like Protein or Xylella fastidiosa Hemagglutinin Protein Bruce Kirkpatrick ......................................................................................................................................... 112 • Inhibition of Xylella fastidiosa Polygalacturonase to Produce Pierce’s Disease Resistant Grapevines Bruce Kirkpatrick ......................................................................................................................................... 121 • Optimizing Grape Rootstock Production and Export of Inhibitors of Xylella fastidiosa Polygalacturonase Activity John M. Labavitch, Ann L.T. Powell, Alan Bennett, Daniel King, and Rachell Booth ...............................127 • Continued Field Evaluation of Diffusible Signal Factor Producing Grape for Control of Pierce’s Disease Steven E. Lindow ......................................................................................................................................... 136 • Field Trial for Resistance to Pierce’s Disease Thomas A. Miller and Matthew Daugherty .................................................................................................145 • Field Evaluation of Grafted Grape Lines Expressing Polygalacturonase-Inhibiting Proteins Ann L.T. Powell and John M. Labavitch .....................................................................................................147 • Tools for Identifying Polygalacturonase-Inhibiting Protein Transmission from Grapevine Rootstock to Scion Ann L.T. Powell, John M. Labavitch, and Abhaya Dandekar......................................................................156 • Greenhouse Evaluation of Grapevine Fungal Endophytes and Fungal Natural Products Antagonistic to Xylella fastidiosa for Control of Pierce’s Disease Philippe Rolshausen and Caroline Roper .....................................................................................................161 • Can Pierce’s Disease PdR1 Resistance Introgressed into Vitis vinifera be Translocated from a Resistant Rootstock to a Susceptible Scion? Drake C. Stenger, David W. Ramming, and Elizabeth E. Rogers ................................................................169 ABSTRACTS • Virulent Phages of Xylella fastidiosa: Prophylactic and Therapeutic Evaluation Carlos F. Gonzalez, Ry Young, Mayukh Das, Tushar Suvra Bhowmick, and Stephen J. Ahern ................173 • Identification of Novel Secreted Virulence Factors from Xylella fastidiosa Elizabeth Rogers and Steven A. Lee ............................................................................................................174 - iii - Section 5: Crop Biology and Disease Epidemiology REPORTS • Blocking Xylella fastidiosa Transmission Rodrigo Almeida and Fabien Labroussaa ....................................................................................................177 • Development of a Grape Tissue Culture and Transformation Platform for the California Grape Research Community David Tricoli ................................................................................................................................................ 184 • Breeding Pierce’s Disease Resistant Winegrapes Andrew Walker ...........................................................................................................................................