25th Annual Meeting Society of Nematologists &®IIIFII ©IFII BARNES, J.M. and J. F. FULKERSON. The impacts of formula funding on the science of nematology. As the land-grant system developed and matured through provision of public lands and long-term, stable funding, so also has the Science of Nematology developed and benefitted. Changes in the scope of nematology research and in amount of formula funding of nematology research since 1961 are described. It is suggested that long-term formula funding has played significant roles in the expansion of nematology faculty positions in state institutions and in the development of graduates in the profession employed in state, federal and industrial organizations. As a result of this funding method, cores of excellence in the fundamental and applied aspects of the science of nematology exist at universities across the U.S. The mandate for adequate support for nematology research in the U.S. is clear; nematode damage occurs in a wide variety of crops including woody species, and their roles in many other problems remain to be clarified. Cooperative States Research Service, U.S. Department 9f. Agriculture, West Auditors Building, Washington, DC 20251. BOLLA, R. I. Nematodes as models for nutritional research. The development of defined media for the in vitro cultivation of several spe- cies of free-living and parasitic nematodes makes it possible to design experiments to investigate nutritional requirements for vitamins, minerals, fatty -acids and other substrates; and to follow the metabolic role for these components. These same studies may be possible by addition and subtraction of nutrients from less well defined media. Experiments using parasitic nemato- des, cultivated in vitro can be designed to investigate host parasite nutri- tional relationships. It may also be possible to select auxotrophic mutants of nematodes which can be used to investigate the metabolic reactions and developmental events for which a particular nutritional sustrate is required. Department of Biology, University of Missouri-St. Louis, St. Louis, MO 63121. BURROWS, PETER M. Interaction concepts for analysis of responses to mixtures of nematode populations. The usual statistical definition of interaction effects is inappropriate for inferences about associative effects of two or more nematode populations in designed experiments containing combinations (mixtures) of population dosages. Alternative formulations based on the product model of Fisher (1923) are suggested. Conceptual and analytical properties of bivariate dose-response surface formulations, with specified marginal responses, are developed. Experimental Statistics Unit, Clemson University~ Clemson, SC 29634-0367. 589 590 Journal of Nematology, Volume l& No. 4~ October 1986 DICKSON, D. W. and JULIA A. MEREDITH. Perspective of nematology teaching. Nematology has reached a crucial point in its development. The number of students enrolled in graduate programs in the United States is lower than previous years in spite of a greater world-wide demand for specialized training in nematology. Few educational centers offer complete programs in the discipline, and most nematology teaching functions as part of other allied subjects. Universities themselves must provide the principal funding for teaching; few other sources are available. Although there has been an increase in didactic materials, they are not necessarily well adapted to specific needs of individual courses. Greater emphasis must be placed on adequate background preparation of students. In addition, proper recruitment and the establishment of comprehensive programs of excellence will determine nematology's future potential to prepare totally trained professionals. University of Florida, Department of Entomology ~ Nematology, Gainesville, FL 32611, U.S.A. DUNN, R. A. Perspective on Extension Nematology. In the broadest sense, every nematologist who has helped any laymen to recognize, better understand, or manage nematode problems has done extension work. Because there are relatively few nematologists and our subject matter is less widely understood than many other sciences, probably more research nematologists are in direct contact with those with nematological problems than are those in most disciplines. However, there is a more formal concept of "extension" to consider. The Cooperative Extension Service was created in 1914 as one of the three strong legs of the Land Grant College System "to aid in diffusing among the people of the United States useful and practical information on subjects relating to agriculture and home economics, and to encourage the application of the same." Many land-grant colleges have included formal assignment of a small percentage of time of one or more nematologists to formal extension work. Fewer than a dozen states have had personnel whose titles included "Extension Nematologist" with a commensurately major fraction of their time assigned to extension work. The steadily increasing complexities of the problems with which agriculture is faced and of the legal, economic, and social environments within which the problems arise and in which the remedies must be applied are creating an environment in which nematologists formally dedicated to extension programs will be increasingly in demand. Dept. of Entomology & Nematology, University of Florida, Gainesville, FL 32611. ENDO, B.Y. Morphology and Ultrastructure of Host Cells Modified for Nematode Feeding. Root morphology is modified during nematode infections in ways that disrupt the vascular flow of water and nutrients and cause severe stunting of crop plants. In infections caused by certain endoparasitic nematodes, multinucleate cells, termed syncytia or giant cells, play an integral role in host susceptibility and resistance. The ultrastructural changes observed in the host resulting from penetration and feeding by nematodes have revealed: I) unique feeding site alterations of the outer wall of syncytia and giant cells; 2) cell wall dissolution and proliferations; 3) secretory products in host cells; and 4) organelle and nuclear changes within the host cells. The concepts of immunochemistry and the utilization of video-enhanced light microscopy supported with electron microscopy should provide ways to better understand host-parasite infections and to develop novel approaches for nematode control strategies. USDA, ARS, Plant Protection Institute, Beltsville Agricultural Research Center, Beltsville, MD 20705. 25th Annual Meeting 591 ESSER, R. P. and G. R. BUCKINGHAM. Genera and species of nema- todes occupyin$ freshwater habitats in North America. A comprehensive list of freshwater nematodes from North America is pre- sented, including the types of water habitats from which they originate. Detailed methods of sample collection, and laboratory processing strate- gies are included. Division of Plant Industry, Bureau of Nematoiogy, Agric. Res. Service USDA, respectively, P.O. Box 1269, Gainesville, FL 32602. EVANS, A.A.F. Diapause as a survival strategy. An obligate diapause has been clearly demonstrated in several populations of Heterodera avenae, Meloidogyne naasi,and strong- ly implicated in Ditylenchus dipsaci. A winter dormancy has been shown in several temperate Heterodera species and a Scot- tish population of Globodera rostochiensis shows a facultative diapause. Delayed maturation and hatch of eggs of several other Meloidogyne species shows different features not necessarily associated with overwintering. The literature shows that the study of diapause in nematodes is still in its descriptive phase. Analysis of its ecological implications can be attempt- ed but more information is needed on environmental factors in facultative diapause and heritability in obligate diapause be- fore approaches can be made towards the underlying physiology. Imperial College at Silwood Park, Ashurst Lodge, Sunninghill, Ascot, Berskhire, SL5 7DE, England. FERRIS, H. EXTRACTION EFFICIENCIES AND POPULATION ESTIMATION. Standardization of data is important in studies of nematode population biology and crop damage. Extraction efficiency has two components: "catch" efficiency, the proportion of the population accessible to the method- ology used; and "separation" efficiency of the technique used to separate nematodes from the surrounding medium. Intrinsic determinants of catch effi- ciency include population age structure, aggregation, and food distribution. Extrinsic determinants include the sample unit selected and the tools used. Intrinsic determinants of separation efficiency include population age structure and activity levels, life history strategy, and ornamentation. Extrinsic factors include soil characteristics, sieve apertures, temperature, and environmental suppressiveness. Most of the factors affecting efficiencies are ascertainable from knowledge of the biology of the organism and the impact of environmental conditions, and by comparing techniques against standards. Techniques vary in reliability, consistency and efficiency; they should be evaluated to test their appropriateness and to allow correction of population estimates to absolute terms. Advancements in biotechnology will provide improvements in diagnostic techniques and population assessments for nema- todes. Division of Nematology, University of California, Davis 95616. 592 Journal of Nematology, Volume 18, No. 4, October 1986 GERBER, K., and G. C. SMART, JR. Nematodes as parasites of aquatic plants. Although many nematode species have been reported to be associated with