Functional proteomics Focus on Post-translational Modifications Introduction ...................................................................................................................................................26 Proteomics – Post-translational modifications Major modifications, related antibodies & enzymes ................27 Phosphorylation : Kinases & Phosphatases Kinases in Cancer ............................................................................................................................................................................................................ 28 Kinases in Neurodegenerative Diseases ......................................................................................................................................................................... 31 Anti-kinases antibodies .................................................................................................................................................................................................... 33 Anti-phosphatases antibodies .......................................................................................................................................................................................... 39 Phosphospecific antibodies ............................................................................................................................................................................................. 40 Active Kinases et Phosphatases for enzyme assays ...................................................................................................................................................... 45 Ubiquitination & Sumoylation Ubiquitination ................................................................................................................................................................................................................... 47 Sumoylation ..................................................................................................................................................................................................................... 49 Ubiquitination & Sumoylation related antibodies ............................................................................................................................................................. 51 Methylation & Acetylation Histone Deacetylases ...................................................................................................................................................................................................... 53 Methylation, Acetlylation and Histone modification related antibodies ............................................................................................................................ 54 Other modifications Functional Proteomics Related antibodies ........................................................................................................................................................................................................... 56 References and sources of information Proteomics - Protein Expression ................................................................................................................58 Protein Expression & Production Peptide synthesis ............................................................................................................................................................................................................. 58 LEXSY protein expression system................................................................................................................................................................................... 59 Protein Expression & Reporting Firefly Luciferase 1-Step Assay Kit, 2 h reading .............................................................................................................................................................. 61 Reporter systems: GFP & lacZ (Renilla GFP vectors, b-Galactosidase Staining Kits) ................................................................................................... 62 Protein Expression & Silencing (siRNA, miRNA) vector based microRNA technology (miRNA) .................................................................................................................................................................. 63 miRNA Analysis with Agilent BioAnalyser ........................................................................................................................................................................ 64 UptiFectin Transfection agent for Protein silencing (siRNA) ............................................................................................................................................ 65 Proteomics - Biochemistry of proteins .....................................................................................................66 Protein preparation Protein Extraction Reagent .............................................................................................................................................................................................. 66 Uptitip™ Titanium ............................................................................................................................................................................................................ 66 IPeX Ab-free Ag Immunoprecipitation Kit ......................................................................................................................................................................... 67 Protein modifications FeBABE ......................................................................................................................................................................................................................... 68 other special modifiers/crosslinkers for 3D structure studies ........................................................................................................................................... 68 ControlledAmine™ conjugation kit ................................................................................................................................................................................... 69 Chromalink-Biotin ............................................................................................................................................................................................................ 69 PEO/PEG Biochemistry : new reagents .......................................................................................................................................................................... 70 Standard and improved fluorescent agents and kits ........................................................................................................................................................ 71 Protein analysis Glycoprotein or sugar fluorescent labeling and analysis ................................................................................................................................................. 72 Colorimetric & fluorimetric Protein assays ....................................................................................................................................................................... 73 BC Assay kit - colorimetric protein assay ......................................................................................................................................................................... 74 LavaPep™ peptide & protein assay kit ............................................................................................................................................................................ 75 Phosphate Assays .......................................................................................................................................................................................................... 76 Innovative electrophoresis: NEXT gels ............................................................................................................................................................................ 77 GeBa Electrophoresis system / pre-cast gels .................................................................................................................................................................. 78 ProSave™ Protein Gel stain ............................................................................................................................................................................................ 79 LavaPurple™ protein gel & blot stain .............................................................................................................................................................................. 80 Blotting glycated proteins with Protran® .......................................................................................................................................................................... 81 One-Step Western Blot Kit, ReliaBLOT™ IP/Western Blot .............................................................................................................................................. 82 UptiLight™, the best of ECL detections ........................................................................................................................................................................... 83 High quality Biochemicals ...............................................................................................................................................................................................