Lectin from Ricinus communis Ricin, B Chain, from RCA60 Product Number L 9639 Storage Temperature 2-8 °C Product Description This lectin product is a solution in 10 mM phosphate Sigma offers a range of lectins suitable for the above buffer, pH 6.5 containing 150 mM NaCl, 10 mM applications. Most Sigma lectins are highly purified by galactose, 0.5 mM dithioerythritol, and 0.02% sodium affinity chromatography, but some are offered as azide. purified or partially purified lectins, suitable for specific applications. Lectins are proteins or glycoproteins of non-immune origin that agglutinate cells and/or precipitate complex Ricinus communis agglutinin should have good carbohydrates. Lectins are capable of binding binding affinity for lactose containing proteins, such as glycoproteins even in presence of various detergents.1 Lactosyl-BSA (Product No. A 5783).2 The agglutination activity of these highly specific carbohydrate-binding molecules is usually inhibited by Many of the lectins are available conjugated to a simple monosaccharide, but for some lectins, di, tri, (conjugation does not alter the specificity of the lectin): and even polysaccharides are required. 1. fluorochromes (for detection by fluorimetry). Lectins are isolated from a wide variety of natural 2. enzymes (for enzyme-linked assays). sources, including seeds, plant roots and bark, fungi, 3. insoluble matrices (for use as affinity media). bacteria, seaweed and sponges, mollusks, fish eggs, body fluids of invertebrates and lower vertebrates, and Please refer to the table for general information on the from mammalian cell membranes. The precise most common lectins. physiological role of lectins in nature is still unknown, but they have proved to be very valuable in a wide This lectin product is purified to apparent variety of applications in vitro, including: homogeneity. SDS-PAGE analysis shows a single band corresponding to Ricin B chain. Ricin B chain 1. blood grouping and erythrocyte polyagglutination has a molecular weight of 33-34 kDa. studies. 2. mitogenic stimulation of lymphocytes. Precautions and Disclaimer 3. lymphocyte subpopulation studies. For Laboratory Use Only. Not for drug, household or 4. fractionation of cells and other particles. other uses. 5. histochemical studies of normal and pathological conditions. Specificity Mitogenic Lectin MW (kDa) Subunits Blood Group Sugar Activity . Abrus precatorius − + Agglutinin 134 4 gal Abrin A (toxin) 60 2 gal Abrin B (toxin) 63.8 2(αβ) gal Agarius bisporus 58.5 − − β-gal(1→3)galNAc Anguilla anguilla 40 2 H α-L-Fuc Arachis hypogaea 120 4 T β-gal(1→3)galNAc Artocarpus integrifolia 42 4 T α-gal→OMe + Bandeiraea simplicifolia BS-I 114 4 A, B α-gal, α-galNAc BS-I-A4 114 4 A α-galNAc BS-I-B4 114 4 B α-gal BS-II 113 4 acq, B, Tk, T glcNAc Bauhinia purpurea 195 4 − β-gal(1→3)galNAc + Caragana arborescens 60; 120a 2/4 − galNAc Cicer arietinum 44 2 − fetuin Codium fragile 60 4 − galNAc Concanavalin A 102 4 − α-man, α-glc + Succinyl-Concanavalin A 51 2 − α-man, α-glc +b Cytisus scoparius − − − galNAc, gal Datura stramonium 86 2(αβ) − (glcNAc)2 Dolichos biflorus 140 4 A1 α-galNAc Erythrina corallodendron 60 2 − β-gal(1→4)glcNAc + Erythrina cristagalli 56.8 2(αβ) − β-gal(1→4)glcNAc Euonymus europaeus 166 4(αβ) B, H α-gal(1→3)gal + Galanthus nivalis 52 4 (h) non-reduc. α-man Glycine max 110 4 − galNAc +c Helix aspersa 79 − A galNAc Helix pomatia 79 6 A galNAc Lathyrus odoratus 40-43 4(αβ) − α-man + Lens culinaris 49 2 − α-man + Limulus polyphemus 400 18 − NeuNAc Bacterial agglutinin − − − galNAc, glcNAc Lycopersicon esculentum 71 − − (glcNAc)3 Maackia amurensis 130 2(αβ) O sialic acid + Maclura pomifera 40-43 2(αβ) − α-gal, α-galNAc Momordica charantia 115-129 4(αβ) − gal, galNAc Naja mocambique mocambique − − − − Naja naja kaouthia − − − − Narcissus pseudonarcissus 26 2 (h) α-D-man Perseau americana − − − − Phaseolus coccineus 112 4 − − Phaseolus limensis 247(II) 8 A galNAc + 124(III) 4 Phaseolus vulgaris PHA-E 128 4 − oligosaccharide + PHA-L 128 4 − oligosaccharide + PHA-P PHA-M - - - - - - - - - - - - - - - - - - - - - - - - - - Table continued on next page - - - - - - - - - - - - - - - - - - - - - - - - - - Specificity Mitogenic Lectin MW (kDa) Subunits Blood Group Sugar Activity Phytolacca americana 32 − − (glcNAc)3 + Pisum sativum 49 4(αβ) − α-man + Pseudomonas aeruginosa PA-I 13-13.7 − − gal +c Psophocarpus tetragonolobus 35 1 − galNAc, gal Ptilota plumosa 65; 170 − B α-gal Ricinus communis Toxin, RCA60 60 2 − galNAc, β-gal Toxin, RCA120 120 4 − β-gal Sambucus nigra 140 4(αβ) − αNeuNAC(2→6)gal +c galNAc a Solanum tuberosum 50; 100 1, 2 − (glcNAc)3 Sophora japonica 133 4 A, B β-galNAc Tetragonolobus purpureas 120(A) 4 H α-L-fuc 58(BA) 2 H α-L-fuc 117(C) 4 H α-L-fuc Triticum vulgaris 36 2 − (glcNAc)2, NeuNAc + Ulex europaeus UEA I 68 − H α-L-fuc UEA II 68 − − (glcNAc)2 Vicia faba 50 4(αβ) − man, glc + Vicia sativa 40 4(αβ) − glc, man + Vicia villosa 139 4 A1+Tn galNAc A4 134 4 A1 galNAc B4 143 4 Tn galNAc Vigna radiata 160 4 − α-gal Viscum album 115 4(αβ) − β-gal Wisteria floribunda 68 2 − galNAc a Concentration-dependent molecular weight b Non-agglutinating and mitogenic c Mitogenic for neuraminidase-treated lymphocytes References 1. Rueben, L., et al., Activities of lectins and their 2. Liener, et al., The Lectins: Properties, Functions, immobilized derivatives in detergent solutions. and Applications in Biology and Medicine, p. 183 Implications on the use of lectin affinity (1986). chromatography for the purification of membrane glycoproteins. Biochemistry, 16, 1787-1794 (1977). IRB/MWM/JRC/NSB/SAG 2/03 Sigma brand products are sold through Sigma-Aldrich, Inc. Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see reverse side of the invoice or packing slip..