European Journal of Taxonomy 131: 1–22 ISSN 2118-9773 http://dx.doi.org/10.5852/ejt.2015.131 www.europeanjournaloftaxonomy.eu 2015 · Strandberg J. & Johanson K.A. This work is licensed under a Creative Commons Attribution 3.0 License. Research article urn:lsid:zoobank.org:pub:DCD171EC-6709-4495-85B3-6430E85C529E New records of Dasyhelea Kieffer, 1911 from Sweden, with descriptions of two new species (Diptera: Ceratopogonidae) Jonas STRANDBERG 1, 2, 3, * & Kjell Arne JOHANSON 1, 4 1 Swedish Museum of Natural History, Department of Zoology, Box 50007, 104 05 Stockholm, Sweden 2 Stockholm University, Department of Zoology, 106 91 Stockholm, Sweden * Corresponding author: [email protected] 3 urn:lsid:zoobank.org:author:EDE10C9E-737E-4F88-9FFF-219BDC80F8BA 4 urn:lsid:zoobank.org:author:F2A38CF6-59EB-4F88-BFEB-761DBEA7B01A Abstract. Based on intensive collecting from various sites in Sweden, the genus Dasyhelea Kieffer, 1911 was reviewed and the number of species now known from the country increased from fi ve to twenty. Among the new species recorded there are two species described as new to science, D. dominiakae sp. nov. and D. gothlandica sp. nov., both in the subgenus Dicryptoscena Enderlein, 1936. The following subgenera are now documented from Sweden: Dasyhelea, Dicryptoscena, Pseudoculicoides Malloch, 1915, Prokempia Kieffer, 1913 and Sebessia Remm, 1979, the two latter subgenera being reported for the fi rst time. Keywords. Biting midges, taxonomy, systematics, new species, Malaise trap. Strandberg J. & Johanson K.A. 2015. New records of Dasyhelea Kieffer, 1911 from Sweden, with descriptions of two new species (Diptera: Ceratopogonidae). European Journal of Taxonomy 131: 1–22. http://dx.doi.org/10.5852/ ejt.2015.131 Introduction The genus Dasyhelea Kieffer, 1911 is classifi ed in the subfamily Dasyheleinae Lenz, 1934 and is cosmopolitan in distribution. More than 600 Dasyhelea species are known, representing one-tenth of all described Ceratopogonidae species in the World (Borkent 2015). The highest diversity is described from large countries in the northern hemisphere, i.e., China, USA and Russia (Borkent 2015). The immature stages of Dasyhelea inhabit a wide range of moist and terrestrial habitats, like peat bogs (Domniak & Szadziewski 2006), rockpools (Wirth 1978), plants of Nepenthes Linnaeus (Wirth & Beaver 1979), tree sap (Domniak 2005) and in mushrooms (Graves & Graves 1985). The short-lived adults (Zilahi- Sebess 1931) are often found in or around the vegetation near the breeding sites (Waugh & Wirth 1976). The structure of the mouthparts in both sexes is similar, i.e., reduction of both mandibles and maxillae (Glukhova 1981), and both males and females feed on nectar and honeydew and according to Wirth (1956) they can act as pollinators of plants such as rubber trees (Hevea brasiliensis (Willd. ex Adr. Juss.)). The most recently described ceratopogonid species from Sweden, which are still valid, are Forcipomyia squamigera Kieffer, 1916 and Bezzia pilipennis Lundström, 1916. Reports thereafter added more species 1 European Journal of Taxonomy 131: 1–22 (2015) records for the country, resulting in a record list of approximately one hundred species of Ceratopogonidae for Sweden (Rehnberg & Brodin 2010; Nielsen et al. 2010; Szadziewski et al. 2012). This may be compared to 184 species in Estonia, 211 in Poland and 256 in Germany (Szadziewski et al. 2012), which indicates that Sweden might have been undersampled historically compared to some other countries. Efforts were therefore made in order to more carefully examine the Swedish diversity using recently trapped material from various sites. The Swedish Dasyhelea include fi ve species previously recorded from Sweden: D. pallidiventris (Goetghebuer, 1931), D. corinneae Gosseries, 1991, D. turfi cola Kieffer, 1925, D. modesta (Winnertz, 1852) and D. notata Goetghebuer, 1920 (Dominiak & Szadziewski 2010). With this report the number of Dasyhelea species in Sweden increases to 20, a low number compared to the more than 60 species recorded from the rest of Europe (Szadziewski et al. 2012). Material & Methods The specimens in this study were collected from Djäknabygds Bokbacke during the summer of 2005, from Limhmans kalkbrott (limestone quarry) during the summer of 2009, and various other localities in Sweden during the spring and summer of 2011. Specimens were collected in Malaise traps and in sweep nets. At the lab they were stored in 80% ethanol in deep freezers. After sorting and preliminary identifi cation, the whole body was cleared using Proteinase K as part of DNA extraction and sequenced for mitochondrial cytochrome I (COI) according to the methods described in Strandberg & Johanson (2010). The COI sequences were used to associate males and females using MEGA 5.2.2 (Tamura et al. 2011), applying the settings in Stur & Borkent (2014). The head, wings and abdomen/genitals from the thorax and one leg from each leg pair were mounted under separate cover slips on microscope slides using Euparal as permanent mounts. Specimens from Limhamns kalkbrott and Bunkefl ostrand are stored at the Museum of Zoology, Lund University, Sweden (MZLU) while the rest are deposited at the Department of Zoology, Swedish Museum of Natural History (NHRS). In the descriptions, specimens were measured for each character and ratios and measurements of the antennae (AR), palpi (PR (III)), wings and costal vein (CR) were obtained following the methods described by Dominiak (2012). Measurements are given for the holotype as well as the range of variation in each character for the paratypes (n = number of specimens examined). Results Class Insecta Linnaeus, 1758 Order Diptera Linnaeus, 1758 Family Ceratopogonidae Newman, 1834 Subfamily Dasyheleinae Lenz, 1934 Genus Dasyhelea Kieffer, 1911 Dasyhelea Kieffer, 1911: 5. Type species: Dasyhelea halophila Kieffer, 1911: 5 (by monotypy). Subgenus Dasyhelea Dasyhelea (Dasyhelea) bensoni Edwards, 1933 Dasyhelea bensoni Edwards, 1933: 91. Dasyhelea vernalis Remm, 1979: 56. New record SWEDEN: 2 ♂♂, Gällivare, Muddus National Park, Lake Muddus, 67.030916° N, 20.254950° E, 418 masl, loc. 036-01, Malaise trap, 7–20 Jun. 2011, leg. H. Gustafsson (NHRS). 2 STRANDBERG J. & JOHANSON K.A., New records of Dasyhelea from Sweden Distribution Great Britain, Norway, Estonia, Romania, Russia (Amur Oblast, Yakutia) (Dominiak & Szadziewski 2010). Biology Specimens were collected on mire close to a lake during mid June. Previously D. bensoni has been collected from the end of May to mid July (Edwards 1933; Remm 1979). Dasyhelea (Dasyhelea) bilineata Goetghebuer, 1920 Dasyhelea bilineata Goetghebuer, 1920: 45. Dasyhelea insignipalpis Kieffer, 1925a: 62 (as D. versicolor var. insignipalpis). Tetraphora saxicola Edwards, 1929: 426. Dasyhelea geleiana Zilahi-Sebess, 1931: 321. Dasyhelea montana Zilahi-Sebess, 1940: 48 (as D. dufouri var montana). Dasyhelea lithotelmatica Strenzke in Thienemann, 1950: 178. Dasyhelea tecticola Remmert, 1953: 334. Culicoides dieuzeidei Vaillant, 1957: 265. Dasyhelea karelica Glukhova & Brodskaya, 1997: 443. New record SWEDEN: 1 ♂, 1 ♀, Malmö, Bunkefl ostrand, Malaise trap, 16 Jul.–1 Aug. 2009, leg. B.W. Svensson (MZLU). Distribution Norway, Finland, Russia, Estonia, Great Britain, Ireland, Belgium, Germany, Poland, Czech Republic, Slovakia, France, Switzerland, Austria, Italy, Croatia, Hungary, Romania, Ukraine (Crimea), Bulgaria, Spain, Gibraltar, Algeria, Turkey (Dominiak & Szadziewski 2010). Biology The specimens were collected in an area with shore meadows on the west coast in the south of Sweden. The larva inhabits shallow water bodies; both natural and artifi cial and preimaginal stages are very tolerant of desiccation and temperature fl uctuations (Zilahi-Sebess 1931; Valkanov 1941; Disney 1975). Remarks The male keys out as D. bilineata in Dominiak (2012). The associated female has the subgenital plate with broad lateral processes and a more hearth-shaped notum in contrast to the isosceles triangular- shaped notum of D. bilineata shown in Dominiak (2012). Dasyhelea (Dasyhelea) caesia Remm, 1993 Dasyhelea caesia Remm, 1993: 193. Dasyhelea lugensis Brodskaya, 1995: 9. New record SWEDEN: 1 ♂, Haninge, Tyresta National Park, Löpanträsk, 59.182483° N, 18.303100° E, 40 masl, loc. 025-07, Malaise trap, 19 Jul.–2 Aug. 2011, leg. J. Strandberg (NHRS). 3 European Journal of Taxonomy 131: 1–22 (2015) Distribution Russia (Leningrad Oblast, Yakutia), Poland (Dominiak & Szadziewski 2010). Biology The specimen was collected in a marsh situated between two lakes with water fl owing through. Last instar larvae and pupae of Dasyhelea caesia have previously been collected from a small body of water and the littoral zone of lakes in Russia during July (Brodskaya 1995; Przhiboro 2005). Dasyhelea (Dasyhelea) malleola Remm, 1962 Dasyhelea malleolus Remm, 1962: 119. New records SWEDEN: 2 ♂♂, 1 ♀, Gällivare, Muddus National Park, Lake Muddus, 67.030916° N, 20.254950° E, 418 masl, loc. 036-01, Malaise trap, 7–20 Jun. 2011, leg. H. Gustafsson (NHRS); 1 ♂, 3 ♀♀, Gällivare, Muddus National Park, Lake Muddus, 67.030916° N, 20.254950° E, 418 masl, loc. 036-02, Malaise trap, 20 Jun.–3 Jul. 2011, leg. H. Gustafsson (NHRS); 2 ♂♂, Gällivare, Muddus National Park, 67.049916° N, 20.256533° E, 443 masl, loc. 037-01, Malaise trap, 7–20 Jun. 2011, leg. H. Gustafsson (NHRS); 1 ♀, Gällivare, Muddus National Park, 67.049916° N, 20.256533° E, 443 masl, loc. 037-02, Malaise trap, 20 Jun.–3 Jul. 2011, leg. H. Gustafsson (NHRS). Distribution Estonia, Norway, Germany, Poland, Czech Republic,