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(n Y"#)%# F 4 (! ) (! ) •/‚ o :; Y" ' . 3V E :W5Q TN 8 :; > =5< :; ' X9 X9 . 3% (5! 5 5X $ Z< ± (5! 5 8 S .; K \ [ ? 8 5. 5% ; D" > =5< :; ' ; ( 5 ?E ( 5 % /'1P 8 R9 * P<0.01 , ** P<0.001 /(! ) P‚ ‚) T 56 Y g ,$A : F5'% . ? ? M (t % g ,$A ' YFT H T pl (!) c % I NA %# 1 , ."# ' 100 ) ED 50 . [ F(3.30 ) = 6.484 , P<0.0016 ] . \ 80 min * 30 / . (p Y"#)%# F 4 (!) (! ) • o \ 60 ** *** 40 G ? G ? $ GH IG% ; B5 E 20 Number of writhes / (••! ••) n) Y•• ' ) T 5••6 Y•• g••, $A : (5 GG? 0 0510 15 =C M (% I g , $A ' YFT H T pl(!) c Norharmane (mg/kg) J%9 D " > =5< :; ' ; ? E :MB [F(4,28 ) = ( (!•) c • (•! •) Pl) • [ K 5% F5'% (! ) •/‚) (~ Y"#) 12.566 , P<0.0001 ] (‚Y"#) 29 ) = 5.149 , P<0.0056 ].[F(3 ((!) /'5 5 N ) (5 % ( 5P / 5 L L) ? ((!) (! ) •) 5% F5'% U" 9 BVQ :W5Q TN Q RS B< 8 S :; ( 5P 29 ) = 3.752 , P<0.0216 ] [F(3 . 3VE :W5Q TN 8 :; > =5< :; ' X9 "D9 . $ ! K (| Y"#) . 3% (5! 5 5X $ Z< ± (5! 5 8 S :; Y" ' .; K \ ] ? 8 5. X9 /'1P 8 R9 * P < 0.05 , ** P< 0.01 , *** P< 0.001 .? ( 5 % j9k< ? 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( 5 % /'1P 8 R9 * P<0.05 , ** P<0.01 Saline (10 ml /kg ) * Flumazenil (2 mg /kg ) Saline (10 ml /kg ) * Harmine (7 mg /kg ) 120 Flumazenil (2 mg /kg ) 100 Harmine + Flumazenil Harmane (10 mg /kg ) ) Harmane + Flumazenil ) 100 min 80 min 30 / 80 30 / 60 * 60 ** 40 40 20 20 Number of writhes Number of writhes 0 0 (5 ? $ H I% B5 `- 5 'A :[ B^ ? $ H I% B5 `- 5 'A :_ B^ G; BG5 (ED 50) G5P / G G5 b G; ( 5 ? G; BG5 (ED 50) G5P / G 5 N ; ? 8 GS :; 5P / '5 5 N `D. ; (5 % 5P / 5 M 8 GS :; 5P /'5 5 N `D. ; (5 % 5P / 5 M ± (5! G5 8 GS :G; Y" ' . " U" 9 8 5. :; Q RS B< ± (5! G5 8 GS :G; Y" ' . " U" 9 8 5. :; Q RS B< G" A ? 8 5. X9 . 3% 5X $ Z< " A ? 8 5. X9 . 3% 5X $ Z< . ; K \ ] . ; K \ [ .? /'1P 8 R9 *P < 0.05 G ( 5 % /'1P 8 R9 ** P < 0.001 *P < 0.01 .? 6 j9k< ? ' / 23 .# / / ' / 0 ! e#$ ()* +& )1 ... ( Writhing ) /? " A %= / " , _ 7 5 , 4 W /( & pI- . (nP)%# =# € D)S .@ g H4A , $ /? 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