Anti-Mono-Methyl-Histone H3 (Arg42) Rabbit pAb Catalog # PTM-610 General Information Images Host species Rabbit Dot Blot Clonality Polyclonal Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Clone number / Primary ab incubation condition: 2 hours at room temperature Synonym H3R42me1 Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Immunogen UniProt MW (kDa) Applications Immunogen peptide quantity: 1 ng, 4 ng, 16 ng species Exposure time: 60 s Human P68431 15 WB The list of peptides is included in the table below: No. Peptide No. Peptide Product Usage Information Lane 1 H3R42me1 Lane 2 H3R17me1 Lane 3 H3R17me2 (sy) Lane 4 H3R17me2 (asy) Application Dilution Recommended species Lane 5 H3R17cit Lane 6 H3R26me1 WB 1:500-1:2000 Human, Mouse, Rat Lane 7 H3R26me2 (sy) Lane 8 H3R26me2 (asy) Lane 9 H3R26cit Lane 10 H3R42me2 (asy) Properties Lane 11 H3R42un Storage Store at -20 °C. Avoid freeze / thaw cycles. WB Stability Stable for 12 months from date of receipt / reconstitution Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:2000 Constituents PBS, Glycerol, BSA Primary ab incubation condition: 2 hours at Purity Antibodies are purified by Protein A conjugated agarose room temperature followed by Mono-methylated H3 (Arg42) peptide affinity Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: MCF-7, C2C12, N2a chromatography. Protein loading quantity: 20 μg Exposure time: 60 s Isotype IgG Predicted MW: 15 kDa Observed MW: 15 kDa Target Information Function Histone post-translational modifications (PTMs) are key Blocking buffer: 5% NFDM/TBST mechanisms of epigenetics that modulate chromatin Primary ab dilution: 1:2000 structures, termed as “histone code”. The PTMs on Primary ab incubation condition: 2 hours at room temperature histone including acetylation, methylation, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) phosphorylation and novel acylations directly affect the Lysate: JK, BRL Protein loading quantity: 20 μg accessibility of chromatin to transcription factors and other Exposure time: 60 s epigenetic regulators, altering genome stability, gene Predicted MW: 15 kDa Observed MW: 15 kDa transcription, etc. Histone methylation occurs primarily at lysine and arginine residues on the amino terminal of core Research Use histones. Methylation of histones can either increase or For research use only, not for use in diagnostic procedures. decrease transcription of genes, depending on which amino acids (Lys or Arg) in the histones are methylated and how many methyl groups are attached (mono-, di-, tri- methylation on Lys, mono-di-symmetric/asymmetric methylation on Arg). Mostly, lysine methylation occurs primarily on histone H3 Lys4, 9, 27, 36, 79 and H4 Lys20, while Arginine methylation occurs primarily on histone H3 Arg2, 8, 17, 26 and H4 Arg3. Histone methylases (HMTs) and histone demethylases (HDMs) are major regulating factors. Cellular location Nucleus APPLICATIONS WB: Western blot IP: Immunoprecipitation CONTACT US China Global IHC-P: Immunohistochemistry-Paraffin ChIP: Chromatin Immunoprecipitation Tel: 400-100-1145 +1 312-802-6843 ICC/IF: Immunocytochemistry/Immunofluorescence FC: Flow Cytometry Email: [email protected] [email protected] MODIFICATIONS ac: acetylated bhb: β-hydroxybutyrylated bu: butyrylated bz: benzoylated cr: crotonylated hib: 2-hydroxyisobutyrylated la: lactylated Web: www.ptm-biolab.com.cn www.ptmbiolabs.com pr: propionylated su: succinylated un: unmodified .