American Association of Veterinary Laboratory Diagnosticians The American Association of Veterinary Laboratory Diagnosticians (AAVLD) is a not-for- profit professional organization which seeks to: • Disseminate information relating to the diagnosis of animal diseases • Coordinate diagnostic activities of regulatory, research and service laboratories • Establish uniform diagnostic techniques • Improve existing diagnostic techniques • Develop new diagnostic techniques • Establish accepted guidelines for the improvement of diagnostic laboratory organizations relative to personnel qualifications and facilities • Act as a consultant to the United States Animal Health Association on uniform diagnostic criteria involved in regulatory animal disease programs AAVLD Officers, 2013 Executive Director Jim Kistler, Navarre, FL President Tom McKenna, Madison, WI President-elect Catherine Barr, Amarillo, TX Vice-president Francois Elvinger, Blacksburg, VA Secretary-Treasurer John Adaska, Tulare, CA Immediate Past-President Tim Baszler, Pullman, WA AAVLD Executive Board, 2013 Executive Director Jim Kistler, Navarre, FL President Tom McKenna, Madison, WI President-elect Catherine Barr, Amarillo, TX Vice-president Francois Elvinger, Blacksburg, VA Secretary-Treasurer John Adaska, Tulare, CA Northeast Sandra Bushmich, Storrs, CT Southeast Lanny Pace, Pearl, MS South-central Bill Johnson, Stillwater, OK North-central Pat Halbur, Ames, IA Northwest Jerry Heidel, Corvallis, OR Southwest Hana Van Campen, Fort Collins, CO Canada Provincial Estela Cornaglia, St. Hyacinthe, QC Canada Federal, ex-Officio Maria Perrone, Ottawa, ON NVSL, ex-Officio Beth Lautner, Ames, IA AVMA, ex-Officio Christine Hoang, Shaumburg, IL AAVLD Secretary-Treasurer's Office PO Box 1770, Davis, CA 95617 Phone 559-781-8900 Fax 559-781-8989 Email: [email protected] Acknowledgments The success of a meeting is a function of both presenters and attendees. A special thank you to all who present their data and findings, all exhibitors and sponsors, and everyone who attends the meeting. We would also like to give a special thank you to all of our invited speakers and moderators for the AAVLD Plenary Session and the USAHA-AAVLD Plenary Session. The Program Committee, listed below, deserves a special acknowledgement for their hard work, organization, review and editing of the abstracts, and moderation of sessions. Kim Grant, from Thomson Reuters, helped us navigate the ScholarOne software. Jackie Cassarly coordinated the meeting room arrangements, exhibitor booth arrangements, sponsor agreements, breaks, and all of the many other details that go into making a meeting a success. ************************************************* 2013 AAVLD Program Committee Scientific Session Moderators Catherine Barr, Chair Marie Culhane Francois Elvinger, Co-chair Francois C. Elvinger Tim Baszler Amy Glaser Ashley Hill Steve Bolin Doreene Hyatt Beate Crossely Bill Layton Scott Fitzgerald Gary Mason Ashley Hill Michelle Mostrom Kathy Kurth Amar Patil Hong Li Karen W. Post Tom McKenna M. D. Salman Durda Slavic Michelle Mostrom Maria Spinato Amar Patil Deepanker Tewari Carlos Reggiardo Belinda S. Thompson David Steffen Deon van der Merwe Patricia A. Talcott Binu Velayudhan Deepanker Tewari Kyoung-Jin Yoon Kyoung-Jin Yoon ************************************************* Please note: Abstracts published in these proceedings were peer reviewed by the Program Committee to determine that data supporting conclusions is likely to be presented, and were edited into a consistent format for publication. Full manuscripts were not evaluated and readers should contact the author for referral to a full presenta- tion of data or for permission to use, copy, or distribute data contained in an abstract. Table of Contents AAVLD Plenary Session Getting Ahead of the Ball: Diagnostic Adaptation in a Global Health Community Saturday, October 19, 2013 Pacific Salon 1,2,3 7:45 AM Welcome and Orientation - Catherine Barr 8:00 AM Interlaboratory Comparison of Equine Herpesvirus type 1 Polymerase Chain Reaction Techniques Utilized in North American Diagnostic Facilities Tim Baszler, Eileen Ostlund, Beate Crossley, Udeni Balasuriya, Dan Bradway, Erdal Erol, Donna Johnson, Dianne Rodman, Steven Sell . .26 8:40 AM H7N9 Emergence, Response, and Projections – Response in the Diagnostic Community Mia Kim Torchetti . .27 9:20 AM Break (45 Min) 10:05 AM Porcine Epidemic Diarrhea Virus’s Emergence in the US: Events, Observations and Opportunities Rodger Main, Eric Burrough, Vickie Cooper, Phillip Gauger, Karen Harmon, Hai T. Hoang, Darin Madson, Kate Mueller, Angela Pillatzki, Kent Schwartz, Wendy Stensland, Greg Stevenson, Dong Sun, Kyoung-Jin Yoon, Jianqiang Zhang . .28 10:45 AM It Takes a Diagnostic Village… Beth Lautner . .29 11:10 AM Panel discussion 20 min Symbols at the end of titles indicate the following designations: # AAVLD Trainee Travel Awardee * Graduate Student Poster Presentation Award Applicant † Graduate Student Oral Presentation Award Applicant + AAVLD/ACVP Pathology Award Applicant ◊ USAHA Paper AAVLD Annual Conference Proceedings 3 San Diego, CA October 2013 Bacteriology 1 Saturday, October 19, 2013 Pacific Salon 3 Sponsor: Thermo Scientific Moderators: Doreene Hyatt, Karen W. Post 1:00 PM Poultry Check MP® MS-MG, a Multiplexed Fluorometric Immunoassay (MFIA) for the Detection of Antibodies to Mycoplasma synoviae and Mycoplasma gallisepticum Martine Bertrand, Isabelle Caya, Andre Ch. Broes . .32 1:15 PM Comparative Evaluation of the TF-Transit Tubes (Biomed) vs. InPouch-TF (Biomed) for the Detection of Tritrichomonas foetus DNA from Bovine Preputial Samples by Real Time PCR Feng (Julie) Sun, Amy Swinford, Pamela J. Ferro, Debabrata Mahapatra, Alesia Reinisch, Anthony Smith, Hemant Naikare . .33 1:30 PM Detection of Bovine Tuberculosis Antibody Response in Sensitized Cattle using the IDEXX M. bovis Antibody Test ◊ Jeffrey T. Nelson . .34 1:45 PM Impact of Blood Sample Storage Time and Temperature on Detection of Bovine Tuberculosis Antibodies using the IDEXX M. bovis Antibody Test ◊ Jeffrey T. Nelson . .35 2:00 PM A Four Plex Real-Time PCR Assay for the Detection and Quantification of Escherichia coli O157 in Cattle Feces * † Lance W. Noll, Pragathi Belagola Shridhar, Xiaorong Shi, Baoyan An, Tiruvoor Nagaraja, Jianfa Bai . .36 2:15 PM Validation of a Broth Microdilution Method and Investigation of in vitro Antimicrobial Susceptibilities of Porcine Brachyspira Species#*† Nandita Mirajkar, Connie Gebhart . .37 2:30 PM Evaluation of MALDI TOF Mass Spectrometry to Identify Brachyspira from Swine Samples Hallie Warneke, Timothy Frana, Joann M. Kinyon, Eric Burrough, Leslie Bower . .38 2:45 PM Detection of Brachyspira in Swine Oral Fluids Hallie Warneke, Timothy Frana, Joann M. Kinyon, Eric Burrough, Bailey L. Wilberts . .39 Symbols at the end of titles indicate the following designations: # AAVLD Trainee Travel Awardee * Graduate Student Poster Presentation Award Applicant † Graduate Student Oral Presentation Award Applicant + AAVLD/ACVP Pathology Award Applicant ◊ USAHA Paper AAVLD Annual Conference Proceedings 4 San Diego, CA October 2013 Epidemiology 1 Saturday, October 19, 2013 Pacific Salon 6,7 Moderators: Ashley Hill, François Elvinger 1:00 PM Zinc Toxicity in Dairy Cattle Alan F. Julian . .42 1:15 PM Copper Overload in Wisconsin Holsteins Doug Lyman . .43 1:30 PM Evaluation of a Commercially-Available ELISA for Detection of ≥500 ppt Aflatoxin M1 in Milk Ashley Hill, Linda Aston, Elizabeth R. Tor, Robert H. Poppenga, Hailu Kinde . .44 1:45 PM Resources for Collecting and Accessioning Single Bulk Milk Samples from Every Commercial Dairy Farm in New York State ◊ Belinda S. Thompson, Paul Virkler, Elizabeth A. Lussier, David Smith . .45 2:00 PM Salmonella Dublin Herd Bulk Tank Seroprevalence of New York Dairy Farms ◊ Belinda S. Thompson, Paul Virkler, Elizabeth A. Lussier, Diane Kilts, David Smith, Bettina Wagner . .46 2:15 PM Beta-hydroxybutyrate Diagnosis of Ketosis in Periparturient Dairy Cattle: Comparison of Blood and Multiple Milk Test Methods for Concordance and Prevalence Estimates in the Same Population of Cows David J. Wilson, Gregory M. Goodell . .47 2:30 PM Comparison of the Ability of a Novel Umbilical Dip, Super7+™ Navel Dip, Verses that of 7% Tincture of Iodine to Desiccate the Umbilical Remnant in Neonatal Holstein Dairy Calves ◊ Julie A. Gard, Soren P. Rodning, Debra Taylor, Sue Duran, Brad Fields, Robin Farrell, Misty Edmondson, Megan Schnuelle, Teri Hathcock, Elizabeth Reed, Rebecca Woodall, Alfred Bearden . .48 2:45 PM Designing a Mobile Application for Field Use on Dairies Experiencing a Toxicologic Event#†◊ Steven Gallego, Hailu Kinde . .49 Symbols at the end of titles indicate the following designations: # AAVLD Trainee Travel Awardee * Graduate Student Poster Presentation Award Applicant † Graduate Student Oral Presentation Award Applicant + AAVLD/ACVP Pathology Award Applicant ◊ USAHA Paper AAVLD Annual Conference Proceedings 5 San Diego, CA October 2013 Pathology 1 Saturday, October 19, 2013 Pacific Salon 1 Sponsor: Biocare Medical Moderators: Gary Mason, Maria Spinato 1:00 PM Dilated Cardiomyopathy in Rio Grande Subspecies of Wild Turkey (Meleagris gallopavo) Jane Kelly, David D. Frame, Leslie McFarlane . .52 1:15 PM Outbreak of Foot and Mouth Disease in Black Bears (Ursus thibetanus) and a Sun Bear (Helarctos malayanus) at a Bear Rescue Facility in Vietnam # Kirsty Officer, Nguyen Thi Lan, Leanne Wicker, Jill Robinson, Panayiotis Loukopoulos . .53 1:30 PM Comparison of the