Provided for non-commercial research and education use. Not for reproduction, distribution or commercial use. The Canadian Journal of Veterinary Research is published by Canadian Veterinary Medical Association. The attached copy is furnished to readers for personal, non-commercial research and education use. Other uses, including reproduction and distribution, or selling or licensing copies, or posting to personal, institutional or third party Web sites are prohibited. Those who require further information regarding reprints or archiving and manuscript policies are encouraged to contact [email protected]. CanadianCanadian JournalJournal ofof VVeterinaryeterinary RResearchesearch RevueRevue CanadienneCanadienne dede RRechercheecherche VVétérinaireétérinaire Articles Comparison of levels and duration of detection of antibodies to bovine viral diarrhea virus 1, bovine viral diarrhea virus 2, bovine respiratory syncytial virus, bovine herpesvirus 1, and bovine parainfluenza virus 3 in calves fed maternal colostrum or a colostrum- replacement product Manuel F. Chamorro, Paul H. Walz, Deborah M. Haines, Thomas Passler, Thomas Earleywine, Roberto A. Palomares, Kay P. Riddell, Patricia Galik, Yijing Zhang, M. Daniel Givens ........................81 Effect of the rider experience and evaluator expertise on subjective grading of lameness in sound and unsound sports horses under saddle Fernando J. Marqués, Cheryl Waldner, Stephen Reed, Fernando Autet, Louise Corbeil, John Campbell ............ 89 Acute exercise does not induce an acute phase response (APR) in Standardbred trotters Lena Kristensen, Rikke Buhl, Katarina Nostell, Lars Bak, Ellen Petersen, Maria Lindholm, Stine Jacobsen ......................... 97 Recovery from desflurane anesthesia in horses with and without post-anesthetic xylazine Turi K. Aarnes, Richard M. Bednarski, Alicia L. Bertone, John A.E. Hubbell, Phillip Lerche ......................... 103 Les biofilms bactériens : leur importance en santé animale et en santé publique Yannick D.N. Tremblay, Skander Hathroubi, Mario Jacques .........................110 Immunogenicity and efficacy of a recombinant adenovirus expressing hemagglutinin from the H5N1 subtype of swine influenza virus in mice Yunpu Wu, Chuanling Qiao, Huanliang Yang, Yan Chen, Xiaoguang Xin, Hualan Chen.....117 Porcine salivary analysis by 2-dimensional gel electrophoresis in 3 models of acute stress: A pilot study María Fuentes-Rubio, José J Cerón, Carlos de Torre, Damián Escribano, Ana M. Gutiérrez, Fernando Tecles .........127 April/Avril 2014, Vol. 78 No. 2 table of contents continued on back cover/ la table des matières se poursuit sur la couverture arrière FOR PERSONAL USE ONLY Established in 1937 ISSN 0830-9000 Canadian Journal of Revue canadienne de Veterinary Research recherche vétérinaire Formerly CANADIAN JOURNAL OF COMPARATIVE MEDICINE Remplace la REVUE CANADIENNE DE MÉDECINE COMPARÉE The journal publishes the results of original research in veterinary La revue publie les résultats de recherches originales en médecine and comparative medicine. Manuscripts must be as concise as pos- vétérinaire et comparative. Les manuscrits doivent être aussi brefs que sible, and the research described must represent a significant con- possible et la recherche décrite doit apporter une contribution impor- tribution to knowledge in veterinary medicine. Full-length papers, tante à la médecine vétérinaire. Les exposés complets, les communi- short communications, and review papers are welcome. All manu- cations brèves et les comptes rendus sont bienvenus. Nous vérifierons scripts will be reviewed for scientific content and editorial accuracy. le fonds scientifique et la forme de tous les manuscrits. Manuscripts must conform to the Instructions for Authors found Les manuscrits doivent être conformes aux directives aux auteurs on the CVMA website. Consultation of a recent issue of the journal qui se trouvent sur le site Web de l’ACMV. La consultation d’un to ensure that the manuscript conforms to current style is also recom- récent numéro de la revue est aussi profitable, afin de s’assurer qu’un mended. Please use the website (www.canadianveterinarians.net) manuscrit se conforme au style courant. Veuillez utiliser le site Web to submit articles. A publication charge of $90 per published page, (www.veterinairesaucanada.net) pour soumettre les articles. Les plus a cost of $10 per table, $15 per illustration, and $150 per color auteurs doivent acquitter des frais de 90 $ par page publiée, 10 $ illustration is payable by contributors. Instructions for ordering pour chaque tableau, 15 $ pour chaque illustration, et 150 $ pour reprints are sent with the galley proofs. Color illustrations will only chaque illustration en couleur. Un formulaire de commande de be published if the author(s) agree to pay the extra cost. tirés à part accompagne l’en voi des épreuves d’un manuscrit. Les illustrations en couleurs ne seront publiées que si les auteurs en assument les frais supplémentaires. Canadian Journal of Veterinary Research Abstracted and/or indexed in:/Résumé et/ou catalogué dans : Revue canadienne de recherche vétérinaire Biological Abstracts Index Veterinarius Editor — Rédacteur Chemical Abstracts Nutrition Abstracts Éva Nagy, Guelph, Ontario Current Contents (Agric. Section) Science Citation Index Associate Editor — Rédacteur adjoint EMBASE/Excerpta Medica Veterinary Bulletin Deborah M. Haines, Saskatoon, Saskatchewan Index Medicus Derwent Veterinary Assistant Editors — Assistants à la rédaction Drug File Serge Messier, Saint-Hyacinthe (Québec) Jeffrey J. Wichtel, Charlottetown, Prince Edward Island Managing Editor — Directrice de la rédaction Heather Broughton, Ottawa, Ontario Assistant Managing Editor — Directrice adjointe à la rédaction Stella Wheatley, Ottawa, Ontario Advertising Manager — Gérante de la publicité Laima Laffitte, Wendover, Ontario Editorial Coordinator/Coordonnatrice de la rédaction Kelly Gray, Ottawa, Ontario Address all correspondence to/Adresser toute correspondance à : Canadian Journal of Veterinary Research Typesetting/Typographie Revue canadienne de recherche vétérinaire AN Design Communications 339, rue Booth Street, Ottawa, Ontario K1R 7K1 www.an-design.ca Tel./Tél. : (613) 236-1162 — Fax/Télécopieur : (613) 236-9681 e-mail/Courriel : [email protected] © Canadian Veterinary Medical Association 2014 L’Association canadienne des médecins vétérinaires 2014 Subscriptions/Abonnements — 2014 Published quarterly by the Canadian Veterinary Medical Association Canada $155 $ ( applicable GST/HST; Publication trimestrielle de l’Association canadienne des TPS/TVH en vigueur) médecins vétérinaires Foreign/Étranger $170 $ US/É.-U. Return undeliverable Canadian addresses to: 339 rue Booth Street, Ottawa, Ontario K1R 7K1, e-mail: [email protected] FOR PERSONAL USE ONLY Article Comparison of levels and duration of detection of antibodies to bovine viral diarrhea virus 1, bovine viral diarrhea virus 2, bovine respiratory syncytial virus, bovine herpesvirus 1, and bovine parainfluenza virus 3 in calves fed maternal colostrum or a colostrum-replacement product Manuel F. Chamorro, Paul H. Walz, Deborah M. Haines, Thomas Passler, Thomas Earleywine, Roberto A. Palomares, Kay P. Riddell, Patricia Galik, Yijing Zhang, M. Daniel Givens Abstract Colostrum-replacement products are an alternative to provide passive immunity to neonatal calves; however, their ability to provide adequate levels of antibodies recognizing respiratory viruses has not been described. The objective of this study was to compare the serum levels of IgG at 2 d of age and the duration of detection of antibodies to bovine viral diarrhea virus 1 (BVDV-1), bovine viral diarrhea virus 2 (BVDV-2), bovine respiratory syncytial virus (BRSV), bovine herpesvirus 1 (BHV-1), and bovine parainfluenza virus 3 (BPIV-3) in calves fed maternal colostrum (MC) or a colostrum replacement (CR) at birth. Forty newborn male Holstein calves were assigned to the CR or the MC group. Group CR (n = 20) received 2 packets of colostrum replacement (100 g of IgG per 470-g packet), while group MC (n = 20) received 3.8 L of maternal colostrum. Blood samples for detection of IgG and virus antibodies were collected from each calf at birth, at 2 and 7 d, and monthly until the calves became seronegative. Calves in the MC group had greater IgG concentrations at 2 d of age. The apparent efficiency of absorption of IgG was greater in the MC group than in the CR group, although the difference was not significant. Calves in the CR group had greater concentrations of BVDV neutralizing antibodies during the first 4 mo of life. The levels of antibodies to BRSV, BHV-1, and BPIV-3 were similar in the 2 groups. The mean time to seronegativity was similar for each virus in the 2 groups; however, greater variation was observed in the antibody levels and in the duration of detection of immunity in the MC group than in the CR group. Thus, the CR product provided calves with more uniform levels and duration of antibodies to common bovine respiratory viruses. Résumé Les produits de remplacement du colostrum sont une alternative pour fournir une immunité passive aux veaux nouveau-nés; toutefois, leur capacité à fournir des niveaux adéquats d’anticorps reconnaissant les virus respiratoires n’a pas été décrite. L’objectif de la présente étude était de comparer les niveaux d’IgG sériques à 2 jours d’âge et la durée de détection des anticorps contre le virus de la diarrhée virale bovine de type 1 (BVDV-1), le virus de la diarrhée