Electronic Supplementary Material (ESI) for Molecular Omics. This journal is © The Royal Society of Chemistry 2018 Supplementary Table 1 Biological Rep 1 Biological Rep 2 Biological Rep 3 Fold change w.r.t Control Fold change w.r.t Control Fold change w.r.t Control % CV No. of No. of No. of Acession ID Description peptides Tm 4-PBA 4-PBA+Tm peptides Tm 4-PBA 4-PBA+Tm peptides Tm 4-PBA 4-PBA+Tm Tm 4-PBA 4-PBA+Tm Cytoplasmic dynein 1 heavy chain 1 OS=Homo sapiens GN=DYNC1H1 Q14204 PE=1 SV=5 195 1.04 1.01 1.04 171 0.96 0.98 0.97 165 1.03 1.02 1.03 4.27 2.06 3.81 DNA-dependent protein kinase catalytic subunit OS=Homo sapiens P78527 GN=PRKDC PE=1 SV=3 185 0.93 1.01 0.96 149 0.93 0.99 0.94 139 0.93 0.97 0.95 0.48 2.03 0.92 Q60FE2 MYH9 variant protein OS=Homo sapiens GN=MYH9 PE=2 SV=1 151 1.16 0.99 1.17 138 1.19 1.01 1.20 137 1.15 1.00 1.19 1.49 0.97 1.46 Q60FE6 Filamin A OS=Homo sapiens GN=FLNA PE=2 SV=1 132 1.07 0.98 1.13 119 1.05 0.98 1.14 108 1.07 0.97 1.14 1.01 0.91 0.65 P49327 Fatty acid synthase OS=Homo sapiens GN=FASN PE=1 SV=3 134 0.80 0.96 0.87 96 0.82 0.94 0.87 87 0.83 0.97 0.88 2.09 1.93 0.80 Spectrin beta non-erythrocytic 1 OS=Homo sapiens GN=SPTBN1 PE=2 B2ZZ89 SV=1 103 0.98 0.99 0.97 90 0.94 0.95 0.93 73 1.02 1.04 1.01 4.15 4.78 4.13 Q00610 Clathrin heavy chain 1 OS=Homo sapiens GN=CLTC PE=1 SV=5 144 0.95 1.00 1.02 127 0.94 0.97 0.94 112 0.96 1.02 0.97 0.88 2.61 3.99 Q9Y490 Talin-1 OS=Homo sapiens GN=TLN1 PE=1 SV=3 114 1.03 0.96 1.08 121 0.96 0.91 0.99 81 1.03 1.02 1.03 3.98 5.81 4.13 Peroxisome proliferator activated receptor interacting complex protein E1NZA1 OS=Homo sapiens GN=PRIC295 PE=2 SV=1 85 0.94 1.02 0.95 65 0.96 1.03 0.98 67 0.93 0.97 0.94 1.53 3.19 2.52 Microtubule-associated protein 1B OS=Homo sapiens GN=MAP1B PE=1 P46821 SV=2 99 1.03 0.97 0.97 70 1.08 1.02 1.11 85 1.05 0.95 1.01 2.24 3.81 6.57 U5 small nuclear ribonucleoprotein 200 kDa helicase OS=Homo sapiens O75643 GN=SNRNP200 PE=1 SV=2 77 0.89 0.94 0.87 79 0.87 0.96 0.93 60 0.84 0.95 0.88 2.62 0.75 3.75 Leucine-rich PPR-motif containing OS=Homo sapiens GN=LRPPRC PE=2 E5KNY5 SV=1 93 0.94 1.02 0.98 91 0.93 1.00 0.94 82 0.93 0.98 0.97 0.62 2.01 2.07 Pre-mRNA-processing-splicing factor 8 OS=Homo sapiens GN=PRPF8 Q6P2Q9 PE=1 SV=2 72 0.88 0.95 0.94 61 0.92 0.97 0.94 64 0.92 1.01 0.95 2.63 3.01 1.13 Neuroblast differentiation-associated protein AHNAK OS=Homo sapiens Q09666 GN=AHNAK PE=1 SV=2 66 1.02 1.07 1.00 61 1.09 1.10 1.04 45 1.05 1.04 1.04 3.27 3.11 2.29 Heat shock protein HSP 90-beta OS=Homo sapiens GN=HSP90AB1 PE=1 P08238 SV=4 142 0.83 0.96 0.88 123 0.87 1.04 0.89 122 1.35 1.22 1.21 28.44 12.42 18.96 O75369 Filamin-B OS=Homo sapiens GN=FLNB PE=1 SV=2 73 1.02 1.01 1.02 67 1.04 1.02 1.09 57 1.03 1.04 1.13 0.61 1.73 5.36 P12814 Alpha-actinin-1 OS=Homo sapiens GN=ACTN1 PE=1 SV=2 78 1.14 0.99 1.12 87 1.14 1.06 1.19 80 1.14 0.98 1.13 0.14 3.86 3.13 P13639 Elongation factor 2 OS=Homo sapiens GN=EEF2 PE=1 SV=4 129 0.87 1.01 0.91 96 0.95 1.06 0.97 93 0.88 0.96 0.93 4.54 5.35 3.57 NUMA1 variant protein (Fragment) OS=Homo sapiens GN=NUMA1 variant Q4LE64 protein PE=2 SV=1 70 0.80 0.95 0.84 57 0.87 0.93 0.86 49 0.81 0.96 0.87 5.09 1.74 1.74 IQ motif containing GTPase activating protein 1 OS=Homo sapiens A4QPB0 GN=IQGAP1 PE=2 SV=1 65 1.03 1.00 1.10 57 0.95 0.93 0.99 50 0.99 1.03 1.01 4.18 5.34 5.85 P09874 Poly [ADP-ribose] polymerase 1 OS=Homo sapiens GN=PARP1 PE=1 SV=4 66 0.87 1.01 0.86 56 0.95 1.08 0.96 54 0.89 0.97 0.92 4.43 5.30 5.26 P14618 Pyruvate kinase PKM OS=Homo sapiens GN=PKM PE=1 SV=4 115 1.01 0.98 1.06 90 0.97 0.94 0.98 97 0.97 0.98 0.98 2.45 2.71 4.26 Bifunctional glutamate/proline--tRNA ligase OS=Homo sapiens GN=EPRS P07814 PE=1 SV=5 55 1.06 0.99 1.01 46 1.07 1.05 1.12 44 1.07 0.97 1.06 0.47 4.09 5.35 Ubiquitin-like modifier-activating enzyme 1 OS=Homo sapiens GN=UBA1 P22314 PE=1 SV=3 79 1.00 1.01 0.99 63 1.01 0.98 0.97 54 1.01 1.05 1.02 0.46 3.45 2.80 E3 ubiquitin-protein ligase HUWE1 OS=Homo sapiens GN=HUWE1 PE=1 Q7Z6Z7 SV=3 42 1.01 0.99 1.03 35 0.99 0.91 0.94 27 0.96 1.05 0.96 2.26 6.77 4.68 78 kDa glucose-regulated protein OS=Homo sapiens GN=HSPA5 PE=1 P11021 SV=2 116 3.47 1.00 2.64 102 3.17 0.96 2.47 106 3.09 1.11 2.63 6.15 7.32 3.59 Q08211 ATP-dependent RNA helicase A OS=Homo sapiens GN=DHX9 PE=1 SV=4 63 0.89 0.96 0.97 61 0.91 0.94 0.95 59 0.94 0.96 0.95 2.91 1.34 1.03 B0V043 Valine--tRNA ligase OS=Homo sapiens GN=VARS PE=2 SV=1 50 0.93 1.04 0.99 31 1.01 1.05 0.98 27 0.93 1.00 0.93 5.08 2.87 3.22 Transitional endoplasmic reticulum ATPase OS=Homo sapiens GN=VCP P55072 PE=1 SV=4 66 1.03 1.03 1.05 66 1.02 0.97 1.01 57 1.03 1.03 1.06 0.60 3.41 2.65 Q9BQG0 Myb-binding protein 1A OS=Homo sapiens GN=MYBBP1A PE=1 SV=2 48 0.92 1.00 0.92 37 0.94 0.96 0.96 32 0.86 0.94 0.85 4.82 3.25 6.08 P30101 Protein disulfide-isomerase A3 OS=Homo sapiens GN=PDIA3 PE=1 SV=4 111 1.91 0.98 1.45 81 1.94 1.01 1.56 85 1.85 1.06 1.65 2.34 3.66 6.20 O00410 Importin-5 OS=Homo sapiens GN=IPO5 PE=1 SV=4 67 1.00 1.03 1.09 44 0.93 0.98 0.94 45 0.94 1.02 0.93 3.57 2.58 9.03 DNA fragmentation factor subunit beta OS=Homo sapiens GN=CAD PE=2 F8VPD4 SV=1 54 0.96 0.93 0.91 32 0.98 1.04 1.00 35 0.96 0.99 0.92 1.06 5.48 5.10 Q14315 Filamin-C OS=Homo sapiens GN=FLNC PE=1 SV=3 54 1.23 1.04 1.16 48 1.16 0.95 1.21 48 1.12 0.98 1.18 5.03 4.47 2.37 P53621 Coatomer subunit alpha OS=Homo sapiens GN=COPA PE=1 SV=2 43 1.02 1.03 1.03 46 1.04 1.07 1.06 40 1.05 0.96 1.04 1.16 5.60 1.45 P12270 Nucleoprotein TPR OS=Homo sapiens GN=TPR PE=1 SV=3 48 0.95 0.95 0.97 37 0.94 0.99 0.97 40 0.98 1.01 0.99 2.31 2.88 1.44 Q86UP2 Kinectin OS=Homo sapiens GN=KTN1 PE=1 SV=1 48 0.98 0.99 0.96 35 1.07 1.07 1.04 32 1.02 0.95 1.01 4.27 6.09 4.01 Isoleucyl-tRNA synthetase, cytoplasmic variant (Fragment) OS=Homo Q59G75 sapiens PE=2 SV=1 42 1.08 0.99 0.99 35 1.02 0.97 1.01 33 1.03 1.09 1.06 3.39 6.61 3.48 Heat shock cognate 71 kDa protein OS=Homo sapiens GN=HSPA8 PE=1 P11142 SV=1 129 0.81 0.97 0.84 122 0.85 1.02 0.89 106 0.71 0.88 0.91 9.37 7.69 4.03 O75533 Splicing factor 3B subunit 1 OS=Homo sapiens GN=SF3B1 PE=1 SV=3 43 0.89 0.96 0.91 33 0.92 1.01 1.00 32 0.93 0.95 0.91 2.59 2.85 5.55 Q5SU16 Beta 5-tubulin OS=Homo sapiens GN=TUBB PE=2 SV=1 283 0.84 1.01 0.94 235 0.84 0.93 0.83 238 0.87 1.00 0.91 1.65 4.72 5.92 P08670 Vimentin OS=Homo sapiens GN=VIM PE=1 SV=4 96 1.19 1.02 1.18 84 1.13 1.02 1.17 87 1.18 1.04 1.28 2.80 1.40 4.97 cDNA FLJ44436 fis, clone UTERU2019706, highly similar to T-complex B3KX11 protein 1 subunit gamma OS=Homo sapiens PE=2 SV=1 53 1.10 1.01 1.15 51 0.91 0.99 0.92 38 0.95 1.01 0.95 10.66 1.28 12.46 EIF4G1 variant protein (Fragment) OS=Homo sapiens GN=EIF4G1 variant Q4LE58 protein PE=2 SV=1 43 0.93 1.00 0.95 39 0.96 1.02 1.00 35 0.86 0.94 0.97 5.55 4.17 2.74 P34932 Heat shock 70 kDa protein 4 OS=Homo sapiens GN=HSPA4 PE=1 SV=4 56 0.93 0.99 0.98 43 0.93 0.98 0.95 33 0.92 1.03 0.97 0.61 2.62 1.89 O14980 Exportin-1 OS=Homo sapiens GN=XPO1 PE=1 SV=1 50 0.94 1.02 1.03 35 0.97 1.03 0.97 36 0.98 1.04 0.98 1.81 0.90 3.18 Proteasome 26S non-ATPase subunit 2 variant (Fragment) OS=Homo Q59EG8 sapiens PE=2 SV=1 54 0.93 0.96 0.98 40 1.02 1.01 1.02 34 0.98 0.96 0.96 4.89 2.73 3.29 ACLY variant protein (Fragment) OS=Homo sapiens GN=ACLY variant Q4LE36 protein PE=2 SV=1 39 0.91 0.93 0.92 35 1.00 1.01 0.98 47 0.92 0.99 0.96 5.29 3.99 3.39 Q6IAT1 GDI2 protein OS=Homo sapiens GN=GDI2 PE=2 SV=1 61 0.92 1.02 0.97 53 0.90 0.98 0.92 41 0.91 1.01 0.95 1.00 2.12 2.69 cDNA FLJ54365, highly similar to DNA replication licensing factor MCM4 B4DLA6 OS=Homo sapiens PE=2 SV=1 41 0.86 1.00 0.89 41 0.88 1.00 0.91 30 0.87 0.94 0.87 1.14 3.10 2.01 P13667 Protein disulfide-isomerase A4 OS=Homo sapiens GN=PDIA4 PE=1 SV=2 82 2.30 1.00 1.85 57 2.27 0.99 1.85 61 2.11 1.07 1.82 4.53 4.03 0.74 P53618 Coatomer subunit beta OS=Homo sapiens GN=COPB1 PE=1 SV=3 35 1.01 1.07 1.00 39 1.06 1.04 1.05 37 1.09 1.03 1.07 3.76 1.84 3.65 X-ray repair cross-complementing protein 5 OS=Homo sapiens GN=XRCC5 P13010 PE=1 SV=3 46 0.95 0.98 0.91 37 0.97 1.00 0.96 33 0.95 0.95 0.94 1.42 2.66 2.78 Q92598 Heat shock protein 105 kDa OS=Homo sapiens GN=HSPH1 PE=1 SV=1 55 0.86 0.97 0.87 43 0.86 1.02 0.90 37 0.91 1.01 0.92 3.31 2.93 2.98 P78371 T-complex protein 1 subunit beta OS=Homo sapiens GN=CCT2 PE=1 SV=4 55 0.93 1.05 0.94 55 0.98 1.00 0.94 51 0.98 1.00 0.99 2.86 3.27 3.02 Q6IBM8 U5-116KD protein OS=Homo sapiens GN=U5-116KD PE=2 SV=1 34 0.95 0.96 0.98 31 0.90 1.03 0.96 29 0.96 1.01 0.98 3.49 3.46 1.23 E7EX90 Dynactin subunit 1 OS=Homo sapiens GN=DCTN1 PE=2 SV=1 33 0.51 0.51 1.17 36 1.41 0.87 1.40 26 0.66 0.92 0.77 55.89 29.01 28.37 Eukaryotic translation initiation factor 3 subunit A OS=Homo sapiens Q24JU4 GN=EIF3A PE=2 SV=1 31 0.95 0.99 0.85 29 0.97 1.03 0.97 26 0.96 0.97 1.00 1.40 3.06 8.20 P55060 Exportin-2 OS=Homo sapiens GN=CSE1L PE=1 SV=3 42 0.93 1.01 1.01 40 0.91 0.99 0.94 37 0.93 1.08 0.97 1.42 4.61 3.57 cDNA FLJ55916, highly similar to General transcription factor II-I OS=Homo B4DH52 sapiens PE=2 SV=1 39 0.83 0.96 0.76 38 0.92 1.03 0.91 33 0.84 0.93 0.84 6.19 5.31 9.45 Epididymis tissue sperm binding protein Li 14m OS=Homo sapiens PE=2 E9KL44 SV=1 49 1.06 1.01 1.08 29 1.06 1.02 1.03 34 1.08 1.03 1.05 1.20 1.08 2.34 Q16531 DNA damage-binding protein 1 OS=Homo sapiens GN=DDB1 PE=1 SV=1 34 0.93 1.03 1.04 25 0.92 1.05 0.95 22 0.89 0.96 0.92 2.42 4.43 6.42 Dihydropyrimidinase-like 2 variant (Fragment) OS=Homo sapiens PE=2 Q59GB4 SV=1 70 0.86 0.98 0.87 56 0.93 0.92 0.98 55 0.92 0.95 0.94 4.17 3.11 5.87 Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens P04406 GN=GAPDH PE=1 SV=3 241 0.99 1.01 0.90 129 1.03 1.03 1.00 131 1.02 0.97 1.01 1.79 3.25 6.18 E9PDF6