DynaPro® Plate Reader™ II User’s Guide M3101 Rev C Copyright © 2014, Wyatt Technology Corporation WYATT TECHNOLOGY Corp., makes no warranties, either express or implied, regarding this instrument, computer software package, its merchantability or its fitness for any particular purpose. The software is provided “as is,” without warranty of any kind. Furthermore, Wyatt Technology does not warrant, guarantee, or make any representations regarding the use, or the results of the use, of the software or written materials in terms of correctness, accuracy, reliability, currentness, or otherwise. The exclusion of implied warranties is not permitted by some states, so the above exclusion may not apply to you. All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, in any form by any means, electronic, mechanical, photocopying, recording, or otherwise, without the prior written permission of Wyatt Technology Corporation. ® DynaPro, DYNAMICS, Wyatt Technology, and its logo are registered trademarks of the Wyatt Technology Corporation. ™ Plate Reader and Protein Solutions are trademarks of Wyatt Technology Corporation. A variety of U.S. and foreign patents have been issued and/or are pending on various aspects of the apparatus and methodology implemented by this instrumentation. Table of Contents Chapter 1: Introduction Overview ............................................................................................................................6 The Instrument ............................................................................................................6 DynaPro Plate Reader II Operations ...........................................................................7 The Software ...............................................................................................................7 How the Manual Is Organized .....................................................................................8 Contacting Wyatt Technology ............................................................................................9 Where to Go from Here .....................................................................................................9 Chapter 2: Safety, Warnings, and Cautions Laser Specifications and Safety Notes ............................................................................ 11 Chapter 3: Installation and Setup Unpacking the Instrument ................................................................................................13 Installing DYNAMICS Software .......................................................................................13 System Requirements ...............................................................................................13 User Accounts with Restricted Privileges ..................................................................14 CD Contents ..............................................................................................................14 Installing the Software ...............................................................................................14 Installing the DynaPro Plate Reader II .............................................................................15 Nitrogen Connection ........................................................................................................16 Chapter 4: Components Front Panel View .............................................................................................................18 Back Panel View ..............................................................................................................19 Top Panel View ................................................................................................................20 Laser ................................................................................................................................21 Laser Beam Warning .................................................................................................21 Laser Enable Standby Time ......................................................................................21 Collecting and Viewing Well Images ................................................................................22 Chapter 5: Using the Display Window Using the Display Tabs ....................................................................................................24 Main Tab ..........................................................................................................................26 Selecting Display Settings for the X, Y Axes .............................................................26 Adjusting the Display Range .....................................................................................27 Setting Temperature ..................................................................................................28 ACF Tab ...........................................................................................................................30 Alarm Tab ........................................................................................................................32 System Tab ......................................................................................................................34 Camera Tab .....................................................................................................................36 Comm Tab .......................................................................................................................37 DynaPro Plate Reader II User’s Guide (M3101 Rev C) 3 Contents Chapter 6: Using the Plate Reader Introduction ......................................................................................................................39 Instrument Warm-up ........................................................................................................39 Choosing the Correct Well Plate and Well Plate Insert ...................................................39 Well Plate Insert Installation ............................................................................................40 Bar Code Labels ..............................................................................................................42 Preparing Samples ..........................................................................................................42 Filtering Samples ......................................................................................................42 Filtering Options ........................................................................................................43 Centrifuging Samples ................................................................................................43 Loading the Well Plate .....................................................................................................44 Loading the sample manually: ..................................................................................44 Loading Well Plates Automatically ...........................................................................45 Loading the Plate Holder .................................................................................................46 Controlling the Plate Reader from DYNAMICS ...............................................................47 Plate Reader Control Buttons ...................................................................................47 Well Labeling Conventions Used By Dynamics ........................................................48 Measuring Automatically .................................................................................................51 Frequently Asked Questions ...........................................................................................52 Chapter 7: Hardware Maintenance General Maintenance ......................................................................................................56 Replacing the Fuses ........................................................................................................57 DynaPro Plate Reader II Firmware Upgrades .................................................................57 Appendix A: Sources for Well Plates Preferred Well Plates .......................................................................................................58 Compatible Well Plates ....................................................................................................59 Appendix B: Technical Specifications Appendix C: Troubleshooting Low Count Rates Appendix D: Connecting to a Network or PC Components ....................................................................................................................64 Instrument connections: ............................................................................................64 LAN connection: ........................................................................................................64 Computer connections: .............................................................................................65 Crossover cable: .......................................................................................................65 Ethernet cable: ..........................................................................................................66 Ethernet to USB adapter: ..........................................................................................66 Ethernet switch: .........................................................................................................67