Braz654 Dent J (2012) 23(6): 654-658 D. Zaparolli et al. ISSN 0103-6440 Effect of Sodium Hypochlorite and EDTA Irrigation, Individually and in Alternation, on Dentin Microhardness at the Furcation Area of Mandibular Molars Danilo ZAPAROLLI Paulo César SAQUY Antonio Miranda CRUZ-FILHO Department of Restorative Dentistry, Ribeirão Preto Dental School, USP - University of São Paulo, Ribeirão Preto, SP, Brazil The aim of this study was to evaluate the effect of irrigation regimens on dentin microhardness at the furcation area of mandibular molars, using sodium hypochlorite and ethylenediaminetetraacetic acid (EDTA), individually and in alternation. The occlusal surface and the roots of 20 non-carious extracted human permanent mandibular molars were cut transversally and discarded. The tooth blocks were embedded in acrylic resin and randomly assigned to 4 groups (n=5) according to the irrigating regimens: 1% NaOCl solution, 17% EDTA solution, 1% NaOCl and 17% EDTA and distilled water (control). Knoop microhardness of dentin at the furcation area was evaluated. Data were analyzed using one-way ANOVA and Tukey’s multiple comparison tests (α=0.05). The results of this study indicated that all irrigation solutions, except for distilled water (control), decreased dentin microhardness. EDTA did not show a significant difference with NaOCl/EDTA (p>0.05), but showed a significant difference with NaOCl (p<0.01). EDTA and NaOCl/EDTA showed a maximum decrease in microhardness. The 17% EDTA solution, either alone or in combination with 1% NaOCl reduced significantly dentin microhardness at the furcation area of mandibular molars. Key Words: irrigating solutions, microhardness, ethylenediamine tetraacetic acid, sodium hypochlorite. INTRODUCTION canals is concerned, no currently available endodontic irrigant fulfills all ideal physicochemical properties The smear layer produced by files and drills during to act simultaneously on the organic and inorganic root canal instrumentation is a film of debris attached to components of smear layer (4). So, the combination of dentin surface, composed by excised dentin particles, auxiliary solutions is necessary to achieve the desired remnants of vital or necrotic pulp tissue, microorganisms outcomes. A recognizably efficient protocol for removal and their byproducts, and retained chemical irrigants of smear layer is the use of sodium hypochlorite (NaOCl) (1). If not removed, the smear layer has a negative in concentrations ranging from 0.5% to 6.15% and 17% impact on root canal adhesion because it will act as a ethylenediaminetetraacetic acid (EDTA) as initial and physical barrier between the filling material and canal final irrigating solutions, respectively (5). walls, undermining sealer penetration and formation of Human dentin is composed by approximately intratubular tags (2). It is also believed that removing this 70% of inorganic material, 20% of organic material layer could dissolve the attached microbiota and their and 10% of water. Ninety percent of the organic matter toxins from root canal walls and reduce the potential of is collagen, which plays a major mechanical role in bacterial survival and reproduction (3). dentin (6). Depletion of the organic phase after root Although several chemical agents are available canal irrigation with NaOCl may cause changes in the with different properties, as far as cleaning of root mechanical properties, including microhardness (7,8). Correspondence: Prof. Dr. Antonio M. Cruz-Filho, Departamento de Odontologia Restauradora, Faculdade de Odontologia de Ribeirão Preto, USP, Avenida do Café, S/N, 14040-904 Ribeirão Preto, SP, Brasil. Tel: +55-16-3602-4792. Fax: +55-16-3633-0999. e-mail: [email protected] Braz Dent J 23(6) 2012 Effect of NaOCl and EDTA on dentin microhardness at furcation 655 However, endodontic irrigants may alter the 5 specimens each, according to the irrigation regimens: physicochemical properties of dentin (3,9). NaOCl 1% NaOCl, 17% EDTA, 1% NaOCl + 17% EDTA and causes oxidation of the organic matrix and denaturation distilled water (control). A standardized volume of 0.5 of collagen, changing the chemical structure of dentin mL of each irrigating solution was delivered directly to and affecting its mechanical properties (10). Oliveira et pulp chamber using an automated micropipette. After 10 al. (11) reported that 1% NaOCl decreased root dentin min, the pulp chamber was copiously rinsed with 10 mL microhardness to depths up to 1,000 µm from the canal of distilled water to remove any residues of the tested lumen. EDTA demineralizes the inorganic components solution. In the group where both chemical irrigants were of dentin by chelating calcium ions, which reduces the used, 0.5 mL of 1% NaOCl was delivered for 10 min, microhardness (12). A previous study showed that 17% followed by aspiration, delivery of 0.5 mL of 17% EDTA EDTA, either alone or in combination with a tensoactive for 10 min and final flush with 10 mL of distilled water. cationic detergent (Cetavlon) caused a more significant After the treatments, the tooth/acrylic resin block sets reduction of root dentin microhardness than 10% citric were bisected longitudinally in a mesiodistal direction acid (13). Cruz-Filho et al. (14) reported that the action in a high-precision cutting machine (Struers; Minitom, of 17% EDTA in decreasing dentin microhardness can Copenhagen, Denmark) and the halves considered the be observed within the first minute after application of most representative of the furcation area were selected. this chelator and that dentin microhardness decreases as The specimens were ground wet with 400-, 500- and the contact time with the solution increases. 600-grit silicon carbide papers and polished with felt There is extensive scientific literature on the discs (Diamond; FGM, Joinvile, SC, Brazil) embedded action of different chelating and demineralizing solutions in aluminum oxide paste (Alpha micropolish LC; Union on the microhardness of root dentin thirds and canal Carbide Corp., Houston, TX, USA) at low speed. The lumen (7,8,11-15). However, little is known about the specimens were washed in running water for 4 h, action of the chelating agents on the microhardness of dried with gauze and examined at ×40 magnification the furcation area of teeth. The aim of this study was to confirm their smoothness. These procedures are to evaluate the effect of irrigation regimens on dentin necessary because measurement of microhardness is microhardness at the furcation area of mandibular only possible on smooth dentin surface. The indentations molars, using 1% NaOCl and 17% EDTA, individually are not visible on non-polished surfaces. and in alternation. The furcation area to be examined (F) was delimited with a #15 scalpel blade by tracing two MATERIAL AND METHODS orthogonal lines, which started from a point (C) located on the outer surface of the root in the most concave The research protocol was approved by portion of the furcation, extending up to the pulp the institutional Ethics Committee (Process chamber floor, towards the entrance of mesial and distal #2010.1.1118.58.9). Twenty human mandibular molars canals (Fig. 1A). Dentin microhardness was measured from the institutional Human Tooth Bank were used in in this region with a Knoop indenter (Shimadzu HMV- the study. Using a water-cooled double-faced diamond 2000; Shimadzu Corporation, Kyoto, Japan) under 10 disc in a high-speed handpiece, the occlusal surface was removed to expose the pulp chamber and the mesial and distal roots were cut transversally approximately 5 mm below the cementoenamel junction and discarded, producing 20 tooth blocks. The pulp tissue remaining in the canal lumen was removed with a Hedströem file (Kerr UK, Peterborough, UK). The canal apical opening was sealed with light-cured composite resin and the access to the pulp chamber was left open. The tooth blocks were stored in sterile saline at 4°C for 24 Figure 1. A: Delimited furcation area, showing the analyzed h and thereafter embedded in self-curing, fast setting region (F), maximum concavity point of the furcation area (C), acrylic resin, except for the occlusal side. The tooth/ composite resin restoration (CR). B: Indentation scheme on the acrylic resin block sets were distributed into 4 groups of furcation area. Braz Dent J 23(6) 2012 656 D. Zaparolli et al. g load and 15 s dwell time. Five indentations spaced by lubricating the dentin canal walls. Final irrigation 200 µm from each other were made in each specimen, with 17% EDTA for 2 min has been recommended for following a direction parallel to the bisecting line removal of smear layer from root canal walls, especially between the orthogonal lines, starting from the pulp at the cervical and middle thirds (12). Therefore, in the chamber floor towards the point “C” (Fig. 1B). The present study, 1% NaOCl and 17% EDTA were used representative dentin microhardness value for each individually and in alternation to evaluate the effect of specimen was obtained as the average of the results for these irrigating regimens on dentin microhardness at the five indentations. Data were analyzed statistically the furcation area. Microhardness determination can by one-way ANOVA and Tukey’s multiple-comparison provide indirect evidence of mineral loss or gain in the test using GraphPad InStat, v.3, software (GraphPad dental hard tissues (16). Knoop indenter microhardness Software Inc., San Diego, CA, USA). A significance test has been used in previous studies to measure dentin level of 5% was set for all analyses. microhardness and the suitability and practicality of this test for evaluating surface changes of dental hard tissues RESULTS treated with chemical agents has been demonstrated (7,12). The Knoop microhardness values (mean ± SD) In the present study, 1% NaOCl reduced for the irrigating regimens are summarized in Table 1. significantly dentin microhardness at the furcation area Statistically significant difference was detected among when compared with distilled water (control group). the irrigating solutions (p<0.0001). The results showed Kinney et al.