Probe List Series Immuno-oncology RNAscope® Probes for Immuno-oncology Get probes for your gene of interest designed and ready to use in two weeks. Featured Publications using Detection of immuno-oncology markers RNAscope® Technology To better stratify patients for immunotherapy treatments, the series of events and biomarkers involved in Differential Expression of the cancer‑immunity cycle need to be better understood. In addition, spatially mapped expression data at Immune-Regulatory Genes the single‑cell level is crucial to understanding the cellular organization and cell‑to‑cell interactions in the Associated with PD-L1 Display in tumor and its complex microenvironment. Melanoma: Implications for PD-1 Pathway Blockade. RNAscope® is a unique RNA in situ hybridization (ISH) technology that provides single‑cell gene expression Taube et. al. Clin Cancer Res. resolution with spatial and morphological context. The RNAscope® assay detects mRNA and long 2015 Sep PMID: 25944800 non-coding RNA in fresh frozen, fresh fixed, and formalin-fixed paraffin-embedded cells and tissues. RNAscope® utilizes a unique probe design strategy that allows for visualization of target RNA as a single In situ Tumor PD-L1 mRNA dot, where each dot is an individual RNA molecule. RNAscope® technology offers the key benefits of high expression is associated with sensitivity, and specificity, resulting in a high signal-to-noise ratio in target gene expression. increased TILs and better outcome in breast carcinomas. Schalper et. al. Clin Cancer Res. RNAscope® target probes for immune markers 2014 May PMID: 24647569 implicated in the cancer-immunity cycle Programmed death ligand-1 Trafficking of T cells to tumors expression in non-small cell CXCL10, CCL3, CCL5, CX3CL1, CX3CR1, lung cancer. CX3CR1, CXCL13, CXCL9 Velcheti et. al. Lab Invest. Priming and activation 2014 Jan;94(1):107-16. 4-1BB (CD137/TNFRSF9), 4. PMID: 24217091 CD27, CD28, CD4, CD70, B CD80 (B7-1), CTLA4, FOXP3, LOO D V Infiltration of GITR (CD357/TNFRSF18), GPR44, ES Increased expression of the SE T cells into tumors HVEM (CD270/TNFRSF14), 3. L 5. immune modulatory molecule IL-12A, IL-12B, IL-2, OX40 EDNRB, ICAM1, ITGAL, SELE, SELP, PD-L1 (CD274) in anaplastic (CD134/TNFRSF4), OX40L VCAM1, VEGF, VEGFA (CD252/TNFSF4), PD-1 E meningioma. D (PDCD1/CD279), PD-L1 (CD274), O N Du et. al. Oncotarget. PD-L2 (CD273), PTGDR, H ® 2014 Dec 31.PMID: 25609200 P PTGDR2-CDS, PTGDS, M RNAscope Assay Y PTGER2, PTGER4, PTGES2, L Spatial resolution of immune marker B7-H1 Expression Model for PTGFR, PTGS1, PTGS2 expression in complex tissue environment Immune Evasion in Human Papillomavirus-Related Cancer antigen 2. One day experiment 6. Recognition Oropharyngeal Squamous Cell presentation & guaranteed performance of cancer cells T Bp50 (CD40/TNFRSF5), U by T cells Carcinoma. M TRAP-1 (CD154), HLA, O Ukpo et. al. Head and Neck R TRAC, TRB, TRG HMGB1, IFNα, IL-10, IL-13, Pathology, 2013 June, IL-4, TLR, TNF-α 7(2):113–21. PMID: 23179191 See more publications at 1. 7. acdbio.com/publications Release of cancer cell antigens Killing of cancer cells AKT1, CDH1, EGFR, IFNγ, IL-6, KRAS, TGFβ,1, TNFα ARG1, ARG2, BTLA (CD272), C10orf54, PD-L1 (CD274), B7-1 (CD80), TIM3 (HAVCR2), IDO1, IDO2, IFNγ, LAG3, MICA, MICB, PD-1 (PDCD1), TGFβ,1, VTCN1, GNLY, FAS (CD95) Simultaneous detection of immune marker mRNA in non-small cell lung cancer (NSCLC) tissue using RNAscope® 2.5 HD Duplex Assay LAG3 / PD‑L1 PD1 / PD‑L1 PD‑L2 / PD‑L1 4-1BB / PD‑L1 CTLA4 / PD‑L1 TIM3 / PD‑L1 DapB negative control NSCLC IL10 / PD‑L1 2 Probe List Detection of immune marker mRNA in cancer samples using RNAscope® 2.5 HD Brown Assay FOXP3 IDO1 Gastric tissue Ovarian tissue CXCL9 CX3CR1 Breast tissue Liver tissue Bp50 TRAP‑1 Lymphoma tissue Lymphoma tissue IL12A IL21R Ovarian cancer Breast cancer Immunotherapy 3 RNAscope® Probes for Immuno‑oncology Targets Probe information, probes for Leica and Ventana platforms and probes for other species are available at www.acdbio.com/probesearch Catalog No. Probe Name Catalog No. Probe Name Catalog No. Probe Name Catalog No. Probe Name 415171 4-1BB (CD137/TNFRSF9) 415181 GITR (CD357/TNFRSF18) 553931 LAG3 454481 TLR9 419211 AKT1 407371 GNLY 427161 MICA 310421 TNF-α 401581 ARG1 606051 GPR44 427181 MICB 433651 TRAC 401591 ARG2 428351 HLA-DRB1 412381 OX40 (CD134/TNFRSF4) 542341 TRAP-1 (CD154) 421471 B7-1 (CD80) 457361 HLA‑e 427201 OX40L (CD252/TNFSF4) 433661 TRB 578471 Bp50 (CD40) 426691 HLA‑y 602021 PD-1 (CD279/PDCD1) 433341 TRG 401601 BTLA (CD272) 434631 HMGB1 410131 PD‑2 (PDCD2) 446351 TRG‑O1 423811 CCL2 319731 HVEM (CD270/TNFRSF14) 600861 PD-L1 (CD274/PDCD1L1) 440371 VCAM1 455331 CCL3 402951 ICAM1 551891 PD-L2 (CD273/PDCD1L2) 311351 VEGF 549171 CCL5 407141 ICOS 407381 PRF1 423161 VEGFA 425261 CCL7 602681 IDO1 415401 PTGDR 418081 VTCN1 (B7-H4) 415451 CD27 401631 IDO2 313761 PTGDR2‑CDS 448871 CD28 472391 IFNα 431471 PTGDS 599391 CD3D 417071 IFNβ1 406791 PTGER2 553971 CD3E 559781 IFNγ 406771 PTGER4 605601 CD4 602051 IL‑10 406781 PTGES2 408061 CD4-40LE-FL 402061 IL‑12 A 462581 PTGFR 419331 CD70 402071 IL-12B 406811 PTGS1 560391 CD8A 586241 IL-13 406801 PTGS2 311091 CDH1 310931 IL-17A 601991 PTPRC (CD45) 310221 CK19 (KRT19) 400301 IL-18 440361 SELE 313001 CSF1 310361 IL-1B 435081 SELP 554341 CTLA4 402041 IL‑2 425601 SISP1(VISTA) 411261 CX3CL1 412201 IL‑20 400881 TGFβ1 411251 CX3CR1 401251 IL‑21 560681 TIM3 (HAVCR2) 311851 CXCL10 400111 IL-33 462711 TLR1 311321 CXCL13 421241 IL-3RA (CD123) 403111 TLR2 440161 CXCL9 315191 IL-4 605951 TLR3 528301 EDNRB 310371 IL- 6 311281 TLR4 310061 EGFR 424251 IL-7 462721 TLR6 427031 FAS (CD95) 415601 ITGAL (LFA‑1) 541441 TLR7 418471 FOXP3 595101 KRAS 542521 TLR8 ACD offers an ever‑growing selection of RNA ISH probes. Don’t see your gene of interest? RNAscope® Made‑to‑Order Target Probes are manufactured within 2 weeks for virtually ANY gene from ANY species in ANY tissue with guaranteed performance. Learn more at www.acdbio.com/target-probes-made-order Visualize other immune markers at www.acdbio.com/immunooncology For Research Use Only. Not for diagnostic use. RNAscope is a registered trademark of Advanced Cell Diagnostics, Inc. in the United States or other countries. All rights reserved. California, USA ©2016 Advanced Cell Diagnostics, Inc. Doc #: MK 51-068/Rev A/06302016.