Hung-Ji Tsai, Ph.D. Johns Hopkins University School of Medicine, Center for Cell Dynamics Room 440, 855 N Wolfe Street, Baltimore, MD 21205 [email protected] and [email protected] EDUCATION AND EXPERIENCE: 2015-pres. Postdoctoral Associate Johns Hopkins University School of Medicine, Baltimore, MD Advisor: Rong Li, Ph.D. Bloomberg Distinguished Professor of Cell Biology Research focus: Effects of aneuploidy on cellular physiology and genome evolution. 2015 Visiting Scientist Stowers Institute for Medical Research Molecular Biology Core, Kansas City, MO Advisor: Karen Staehling, Ph.D. Research focus: Automated high-throughput screen (HTS) using robotic platforms. 2013-2015 Postdoctoral Fellow Stowers Institute for Medical Research, Kansas City, MO Advisor: Rong Li, Ph.D. Research focus: Genetic dependency and genome structure in aneuploidy 2007-2013 Ph.D. in Molecular, Cellular, Developmental Biology and Genetics University of Minnesota, Minneapolis, MN Advisor: Judith Berman, Ph.D. Research focus: Genome organization in Candida albicans. 2004 M.S. in Microbiology National Taiwan University School of Medicine, Taipei, Taiwan Advisor: Shu-Chun Teng, Ph.D. Research focus: Molecular mechanisms of telomere-telomere recombination. 2002 B.A. in Agriculture Chemistry National Taiwan University, Taipei, Taiwan Undergraduate Research Advisor: Shu-Chun Teng, Ph.D. Research focus: Protein interactions in telomere lengthening regulation. HONORS AND AWARDS: 2015 Young Investigator Award, for the research proposal: Targeting karyotypic heterogeneity in aneuploidy, Prostate Cancer Foundation, Class of 2016 2012 Schmit-Steer Award, Minnesota Medical Foundation 2011 Milne-Brandenburg Travel Award, University of Minnesota 2010 Microbial and Plant Genomics Institute Travel Award, University of Minnesota 2006 Excellent Dissertation Award, for outstanding Master thesis research, Academic Foundation of Professor Jung-Yaw Lin Hung-Ji Tsai, Ph.D. 2002 Distinction Student Service Education Award, for outstanding extra curriculum service, National Taiwan University EXTRAMURAL RESEARCH SUPPORT: 2016-2019 Young Investigator Award - Prostate Cancer Foundation, 75,000 USD per year TEACHING EXPERIENCE: 2010-2016 Research Mentor, for graduate, undergraduate students in Berman and Li Laboratories at the University of Minnesota, Stowers Institute, University of Missouri and Johns Hopkins University (3/5 won university-wide undergraduate research awards) 2008-2010 Teaching Assistant Department of Genetics, Cell Biology, and Development, University of MN, Minneapolis, MN - Genetics (office hours and lectures) - Cell Biology (office hours) INVITED SEMINARS: 2018 Mid-Atlantic Mitosis and Meiosis Meeting 2018, - American Society for Cell Biology meeting Topic: Genetic commonality and dependency in a karyotypically heterogeneous aneuploid population. 2017 Baltimore Fungal Biology Center, Baltimore MD Topic: Aneuploidy associated cellular stress 2016 Genetics Society of America Yeast Genetics Meeting 2016, Orlando, FL Topic: Fitness landscape driven by the interaction between gene mutation and aneuploidy 2014 Graduate Institute of Microbiology. Taipei, Taiwan Topic: Genome-wide studies of replication origins in Candida albicans reveal their conserved and distinct features. PUBLICATIONS: Peer review articles 2018 Tsai, H.J., Nelliat, A.R., Choudhury, M.I., Bradford, W.D, Cook, M.E., Kim, J., Sun, S.X., Schatz, M.C., and Li, R. Hypo-Osmotic-Like Stress Underlies General Cellular Defects of Aneuploidy. Nature (3rd revision under review: 2 reviewers have agreed for publication, manuscript upon request) 2018 Zhu, J., Tsai, H.J., Gordon, M.R., and Li, R. Cellular Stress Associated with Aneuploidy. Developmental Cell. 44(4):420-431 Hung-Ji Tsai, Ph.D. 2017 Mulla, W.A., Seidel, C.W., Zhu, J., Tsai, H.J., Smith, S., Pushpendra, S., Bradford, W.D., McCroskey, S., Nelliat, N.R., Conkright, J., Peak, A., Malanowski, K., Perera, A., and Li, R. Aneuploidy causes epigenetic instability and loss of mating type speciation in Saccharomyces cerevisiae. eLife 2017;6:e27991 DOI: 10.7554/eLife.27991 2015 Chen, G., Mulla, W.A., Kucharavy, A., Tsai, H.J., Rubinstein, R., Conkright, J., McCroskey, S., Bradford, W.D., Weems, L., Haug, J.S., Seidel, C.W., Berman, J., and Li, R. Targeting the adaptability of heterogeneous aneuploids. Cell. 160(4):771-84 2014 Tsai, H.J., Baller, J.A., Liachko, I., Koren, A., Burrack, L.S., Hickman, M.A., Thevandavakkam M.A., Rusche, L.N., and Berman, J. Origin replication complex binding, nucleosome depletion patterns, and a primary sequence motif can predict origins of replication in a genome with epigenetic centromeres. mBio 5(5):e01703-14. 2013 Furniss, K.L., Tsai, H.J., Byl, J.A., Lane, A.B., Vas, A.C., Hsu, W.S., Osheroff, N., and Clarke, D.J. Direct monitoring of the strand passage reaction of DNA topoisomerase II triggers checkpoint activation. PLoS Genetics. 9(10): e1003832 2011 Hsu, W.S., Erickson, S.L., Tsai, H.J., Andrews, C.A., Vas, A., and Clarke, D.J. S-phase cyclin- dependent kinases and Pds1 collaborate to preserve sister chromatid cohesion during DNA replication in budding yeast. Mol Cell Biol. 12:2470-2483. 2010 Koren, A., Tsai, H.J., Tirosh, I., Burrack, L.S., Barkai, N., and Berman, J. Epigenetically-inherited centromere and neocentromere DNA replicates earliest in S-phase. PLoS Genetics. 6:e1001068. 2010 Giménez-Abián, J.F., Díaz-Martínez, L.A., Beauchene, N.A., Hsu, W.S., Tsai, H.J., Clarke, D.J. Determinants of Rad21 localization at centrosome in human cells. Cell Cycle. 9:1759-1763. 2010 Beauchene, N.A., Díaz-Martínez, L.A., Furniss, K., Hsu, W.S., Tsai, H.J., Chamberlain, C., Esponda, P., Giménez-Abián, J.F., Clarke, D.J. Rad21 is required for centrosome in human cells independently of its role in chromosome cohesion. Cell Cycle. 9: 1774-1780. 2010 Huang, Y.C., Tseng, S.F., Tsai, H.J., Lenzmeier, B.A., Teng, S.C. Direct interaction between Utp8p and Utp9p contributes to rRNA processing in budding yeast. Biochem Biophys Res Commun. 393(2): 297-302. 2009 Tseng, S.F., Shen, Z.J., Tsai, H.J., Lin, Y.H., Teng, S.C. Rapid Cdc13 turnover and telomere length homeostasis are controlled by Cdk1-mediated phosphorylation of Cdc13. Nucleic Acids Res. 37(11): 3602-2611. 2006 Tsai, H.J., Huang, W.H., Li, T.K., Tsai, Y.L., Wu, K.J., Tseng, S.F., Teng, S.C. Involvement of topoisomerase III in telomere-telomere recombination. J Biol Chem. 281(19): 13717-13723. 2006 Wen, W.Y., Tsai, H.J., Lin, C.C., Tseng, S.F., Wong, C.W., Teng, S.C. Telomere configuration influences the choice of telomere maintenance pathways. Biochem Biophys Res Commun. 343(2): 459-466. 2003 Teng, S.C., Tsai, H.J., Tsai, M.G., Lee, W.M., Chen, I.C., Lin, C.C. Using both chemical and biological fingerprints for the quality study of estrogenic licorice (Glycyrrhiza uralensis). J Food Science. 68: 2372-2377. Hung-Ji Tsai, Ph.D. Review articles 2005 Tsai, H.J., Huang, W.H., Lu, C.Y., Chen, Y.C., Teng, S.C. Werner and Bloom syndromes. Formosan Journal of Medicine. 12: 544-51. PROFESSIONAL SOCIETIES AND EDITORIAL SERVICE: 2018-present eLife Ambassador 2016-present Genetics Society of America 2012-present Ad hoc reviewer – PLoS One, Genome Research, Scientific Report 2010-2012 American Society of Microbiology 2012-2013 American Society of Cell Biology 2011-2016 Associate Faculty member for Faculty of 1000 PROFESSIONAL SERVICE: 2004-2005 Noncommissioned officer in compulsory Taiwan military service References: Dr. Judith Berman, Professor, Tel Aviv University Department of Molecular Microbiology & Biotechnology George Wise Faculty of Life Sciences Ramat Aviv 69978, Israel. +(972) 3-640-7633 [email protected] Dr. Rong Li, Professor, Johns Hopkins University School of Medicine Director of Center for Cell Dynamics Department of Cell Biology 440 Rangos Building, 855 N Wolfe St. Baltimore, MD 21205 410-955-9938 [email protected] Dr. Arturo Casadevall, Bloomberg Distinguish Professor, Johns Hopkins University School of Public Health Chair, Molecular Microbiology and Immunology Department of Molecular Microbiology and Immunology Room E5132, 615 N. Wolfe Street, Baltimore, Maryland 21205 410-955-3457 [email protected] Dr. Duncan Clarke, Associate Professor, University of Minnesota Department of Genetics, Cell Biology, and Development 160 Church St., Minneapolis, MN 55455 612-624-3442 [email protected] .