Entomology Publications Entomology 1999 Genetic similarity among pheromone and voltinism races of Ostrinia nubilalis (Hubner) (Lepidoptera: Crambidae) P.C.R.G. Marcon University of Nebraska-Lincoln D. B. Taylor University of Nebraska-Lincoln C. E. Mason University of Delaware Richard L. Hellmich Iowa State University, [email protected] B. D. Siegfried University of Nebraska-Lincoln Follow this and additional works at: http://lib.dr.iastate.edu/ent_pubs Part of the Entomology Commons The ompc lete bibliographic information for this item can be found at http://lib.dr.iastate.edu/ ent_pubs/79. For information on how to cite this item, please visit http://lib.dr.iastate.edu/ howtocite.html. This Article is brought to you for free and open access by the Entomology at Iowa State University Digital Repository. It has been accepted for inclusion in Entomology Publications by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. Genetic similarity among pheromone and voltinism races of Ostrinia nubilalis (Hubner) (Lepidoptera: Crambidae) Abstract The eg netic variability of seven European corn borer populations, Ostrinia nubilalis, from North America and Europe was assessed by polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) analysis and DNA sequencing. The uclen ar ribosomal internal transcribed spacer 1 (ITS-1) region (≈ 500 base pair [bp]) and four mitochondrial (mtDNA) regions (1550 bp total) were examined. The ms artweed borer, Ostrinia obumbratalis, and south-Western corn borer, Diatraea grandiosella, were used for comparisons. Of 106 restriction sites identified (80 in mtDNA and 26 in ITS-1), none differentiated geographical populations, pheromone races, or voltine ecotypes of the European corn borer. The al ck of variation in the ITS-1 of European corn borer was confirmed by DNA sequence analysis. The eg netic similarity of European corn borer populations, despite their wide geographical range and physiological differences, may be explained by a relatively recent origin for the voltinism and pheromone races, gene flow among races, and/or expansion from genetic bottlenecks. Keywords internal transcribed spacer, mitochondrial DNA, Ostrinia nubilalis, pheromone races, voltinism ecotypes, Midwest Livestock Insects Research Laboratory, Corn Insects and Crop Genetics Research Unit Disciplines Entomology Comments This article is from Insect Molecular Biology; 8 (1999);213-221; doi:10.1046/j.1365-2583.1999.820213.x . Rights Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The onc tent of this document is not copyrighted. This article is available at Iowa State University Digital Repository: http://lib.dr.iastate.edu/ent_pubs/79 .