Curriculum Vitae Amnon Kohen Department of Chemistry Tel: (319) 335-0234 University of Iowa FAX: (319) 335-1270 Iowa City, IA 52242 [email protected] EDUCATION AND PROFESSIONAL HISTORY Education D.Sc., Chemistry 1989-1994 Technion – Israel Institute of Technology, Haifa, Israel Advisor: Professor T. Baasov Topic: Mechanistic Studies of the Enzyme KDO8P Synthase B.Sc., Chemistry (with Honors) 1986-1989 Hebrew University, Jerusalem, Israel Positions Professor 2010-Present Department of Chemistry, University of Iowa, Iowa City, IA and Molecular and Cellular Biology Program, and MSTP faculty member Associate Professor 2005-2010 Department of Chemistry, University of Iowa, Iowa City, IA and Molecular and Cellular Biology Program Assistant Professor 1999-2005 Department of Chemistry, University of Iowa, Iowa City, IA Postgraduate Researcher 1997-1999 With Professor Judith Klinman Department of Chemistry, University of California, Berkeley Topic: Hydrogen Tunneling in Biology: Alcohol Dehydrogenases Postgraduate Fellow 1995-1997 With Professor Judith Klinman Department of Chemistry, University of California, Berkeley Topic: Hydrogen Tunneling in Biology: Glucose Oxidase Visiting Scholar Fall 1994 With Professor Karen S. Anderson Department of Pharmacology, Yale Medical School, New Haven, CT Kohen, A. Affiliations American Society for Biochemistry and Molecular Biology (ASBMB, 2013 - present) Center for Biocatalysis and Bioprocessing (CBB) University of Iowa (2000-Present) The Interdisciplinary Graduate Program in Molecular Biology, University of Iowa (2003- Present) American Association for the Advancement of Science (AAAS, 2011-present) American Chemical Society (1995-Present) Divisions: Organic Chemistry, Physical Chemistry, Biochemistry Sigma Xi (1997-Present) Protein Society (1996-1998) Honors and Awards • Career Development Award (University of Iowa- 2015-2016) • Graduate College Outstanding Faculty Mentor Award (2015). • The Lady Davis Visiting Professorship (The Lady Davis Fellowship Trust, Israel – 2009) • Faculty Scholars Award (University of Iowa- 2007-2010) • Career Development Award (University of Iowa- 2006-2007) • NSF Faculty Early Career Development (CAREER) Award (2002-2007) • Wolf Foundation Award for excellence in doctoral studies (1992) • Miriam and Aaron Gutwirth Award for excellence in graduate studies (1991) • Amos-De-Shalit Scholarship for distinguished undergraduate students (1987) • Hebrew University Dean’s List (1987-1989) SCHOLARSHIP Publications All the publication below are in a peer reviewed journals and books. (* = Corresponding author, senior author, major contribution) Publications resulting from work performed at Iowa: 1. Morris, H., Singh, P., Kohen, A.,* Tivanski, A.,* “Probing Single Molecule Enzyme-Drug Interaction with Molecular Recognition Force Spectroscopy:., Submitted to J. Am. Chem. Soc. April 7, 2015. 2. Francis, K., Kohen, A.,* “Dihydrofolate Reductase as a Model for Enzyme Catalysis”, Current Biotech., 4, 1-10, 2015. (Invited review). 3. Singh, P., Francis, K., Kohen, A.,* “Network of remote and local protein dynamics in dihydrofolate reductase catalysis”, ACS Cat., 5, 3067–3073, 2015. 4. Guo, Q., Pagano, P., Li, Y., Kohen, A., Cheatum, C.M.,* “Line Shape Analysis of Two-dimensional Infrared Spectra”, J. Chem. Phys., 142, 212427, 2015. PMCID: PMC4409623 5. Ghosh, A.K., Islam, Z., Krueger, J.D., Abeysinghe, D.T., Kohen, A.,* “The General Base in the Thymidylate Synthase Catalyzed Proton Abstraction”, Phys. Chem. Chem. Phys. Accepted for publications, Just accepted articles on line, April 15, 2015. 2 Kohen, A. 6. Świderek, K., Kohen, A., Moliner, V.,* “Influence of Active Site Conformations on the Hydride Transfer step of the Thymidylate Synthase Reaction Mechanism”, Phys. Chem. Chem. Phys. Accepted for publication April 1, 2015. 7. Abeysinghe, T., Kohen, A.,* “Role of Long-Range Protein Dynamics in Different Thymidylate Synthase Catalyzed Reactions”, Int. J. Mol. Sci., 16, 7304-7319, 2015. Special Issue on Protein and Protein- Lingand Interactions. 8. Nilaweera, T., Saeed, M. and Kohen, A.,* “Targeting the de novo Biosynthesis of Thymidylate for the Development of PET Probe for Pancreatic Cancer Imaging”, Biochemistry, 54, 1287–1293, 2015. Chosen to be highlighted on the journal’s home page: http://pubs.acs.org/journals/bichaw/index.html. 9. Singh, P., Abeysinghe, T., Kohen, A.,* “Linking Protein Motion to Enzyme Catalysis”, Molecules, 20, 1192-1209, 2015. (Invited review) 10. Doron, D., Stojković, V., Gakhar, L., Kohen, A., Major, D.,* “Free Energy Simulations of Active-Site Mutants of Dihydrofolate Reductase”, J. Phys. Chem. B., 2015, 119, 906–916. 11. Kohen, A.,* “Role of Dynamics in Enzyme Catalysis: Substantial vs. Semantic Controversies”, Acc. Chem. Res., 2015, 48, 466–473. (Special Issue on “Enzymes Dynamics and Catalysis”) 12. Singh, P., Morris, H., Tivanski, A.V., Kohen, A.,* “Determination of concentration and activity of immobilized enzymes”, Submitted to Anal., Biochem., Accepted for publication Feb. 12, 2015. http://dx.doi.org/10.1016/j.ab.2015.02.014 13. Singh, P., Morris, H., Tivanski, A.V., Kohen, A.,* “A calibration curve for immobilized dihydrofolate reductase activity assay”, Data in Brief, 2015, 4, 19-21. 14. Mishanina, T., Kohen, A.,* “Synthesis and application of isotopically labeled flavin nucleotides”, Submitted to J. Labeled Comp. Radiopharma., Accepted for publication with minor revision Dec. 26, 2014. 15. Liu, C.T.,‡ Francis, K.,‡ Layfield , J., Huang, X., Hammes-Schiffer, S., Kohen, A.,* Benkovic, S.J.,* “The Escherichia coli Dihydrofolate Reductase Catalyzed Proton and Hydride Transfers: Order and the Roles of Asp27 and Tyr100”, Proc. Nat., Acad. Sci. USA, 2014. 111, 18231–18236. 16. Klinman, J.P.,* Kohen, A,* “Evolutionary Aspects of Enzyme Dynamics”, J. Biol. Chem., 2014. 289, 30205-30212. 17. Mishanina, T., Corcoran, J.M., Kohen, A.,* “Substrate Activation in Flavin-Dependent Thymidylate Synthase”, J. Am. Chem. Soc., 2014, 136, 10597–10600. 18. Roston, D., Kohen, A., Doron, D., Major, D.T.,* “Simulations of Remote Mutants of Dihydrofolate Reductase Reveal the Nature of a Network of Residues Coupled to Hydride Transfer”, J. Comp. Chem., 2014, 35, 1411-1417. 19. Islam, Z., Strutzenberg, S.T., Gurevic, I., and Kohen, A.*, “Concerted versus Step-Wise Mechanism in Thymidylate Synthase”, J. Am. Chem. Soc. 2014,136, 9850–9853. 20. Wang, Z., Singh, P., Czekster, C., Kohen, A.,* Schramm, V.L.,* “Protein Mass-modulated Effects in the Catalytic Mechanism of Dihydrofolate Reductase: Beyond Promoting Vibrations”, J. Am. Chem. Soc., 2014, 136, 8333-8341. 21. Doron, D., Kohen, A., Nam, K., Major, D.T.,* “How Accurate are Transition States from Simulations of Enzymatic Reactions?” J. Chem. Theory Comput., 2014, 10, 1863-1871. 3 Kohen, A. 22. Francis, K., Kohen, A.,* “Protein Motions and the Activation of the C-H Bond Catalyzed by Dihydrofolate Reductase”, Curr. Opinion Chem. Biol., 2014, 21, 19–24. (Invited Review). 23. Singh, P., Sen, A., Francis, K., Kohen, A.,* “Extension and limits of the network of coupled motions correlated to hydride transfer in dihydrofolate reductase”, J. Am. Chem. Soc., 2014, 136, 2575–2582. 24. Roston, D., Kohen, A.,* “Stereospecific Multiple Isotopic Labeling of Benzyl Alcohol”, J. Labeled Comp. Radiopharma., 2014, 57, 75-77. 25. Roston, D., Islam, Z., Kohen, A., “Kinetic Isotope Effects as a Probe of Hydrogen Transfers to and from Common Enzymatic Cofactors”, Archives Biochem. Biophys, 2014, 544, 96-104. 26. Francis, K., Kohen, A., “Standards for the Reporting of Kinetic Isotope Effects in Enzymology”, Perspectives in Science, 2014, 1, 110–120. A STRENDA and Beilstein book, Eds. Cornish-Bowden, A., and Cattenr, C., Pub. Elsevir. (Invited Chapter). 27. Francis, K., Stojković, V., Kohen, A.,* “Preservation of Protein Dynamics in Enzyme Evolution“, J. Biol. Chem., 2013, 288, 35961-35968. 28. Roston, D., Kohen, A.,* “A Critical Test of the “Tunneling and Coupled Motion” Concept in Enzymatic Alcohol Oxidation”, J. Am. Chem. Soc., 2013, 135, 13624–13627. 29. Islam, Z, Roston, D., Kohen, A., “Isotope effects as probes for H-tunneling and enzyme dynamics”, Special Issue on "Isotope Effects" in Molecules, Ed. Hansen, P.E., 2013, 18, 5543-5567. (Invited Review). 30. Wang, Z., Sapienza, P.J., Abeysinghe, T., Luzum, C., Lee, A.L., Finer-Moore, J.S., Stroud, R.M., and Kohen, A.,* “Mg2+ binds to the surface of thymidylate synthase and affects hydride transfer at the interior active site”, J. Am. Chem. Soc., 2013. 135, 7583-7592. 31. Wang., Z., Ferrer, S., Moliner, V.,* Kohen, A.,* “QM/MM calculations suggest a unique mechanism for the proton abstraction catalyzed by thymidylate synthase”, Biochemistry, 2013, 52, 2348-2358. 32. Kohen, A, “Positron emission tomograpgy tracer“, US 2013/0034496 A1, Patent application published Feb. 7, 2013. 33. Cheatum, C.M.,* Kohen, A,* “Relationship of Femtoseconds Dynamics to the Catalyzed Hydrogen Transfer” Topics in Current Chemistry, Special issue on “Macromolecular Dynamics in Biological Catalysis”, Eds, Hammes-Schiffer, S. & Klinman, J.P., Pub: Elsevier, (Invited Review). Published on-line March 28, 2013. 34. Kohen, A.*, Klinman, J.P.*, “Hydrogen Tunneling Links Protein Dynamics to Enzyme Catalysis”, Ann. Rev. Biochem., 2013, 82, 471–496. (Invited Review). 35. Wang, Z., Abeysinghe, T., Finer-Moore, J.S., Stroud, R.M., Kohen, A.*, “A Remote Mutation Affects the Hydride Transfer by Disrupting Concerted Protein Motions in Thymidylate Synthase”, J. Am. Chem. Soc., 2012, 134, 17722-17730. 36. Vardi-Kilshtain, A.,