R. Kühn, W. Wurst (Eds.) Gene Knockout Protocols Series: Methods in Molecular Biology, Vol. 530 ▶ Comprehensive guide to all techniques required for the generation of classical and conditional knockout mouse strains ▶ Includes techniques for ES cell establishment, ES cell in vitro differentiation, and nuclear cloning ▶ Provides a systematic overview of techniques for mutant phenotyping ▶ Covers all gene knockout techniques, including gene targeting, chemical, gene trap, and transposon mutagenesis ▶ Written by hands-on experts who have optimized each method for experimental success 2nd ed. 2009, XVI, 516 p. With the completion of the mouse and human genome sequences, the functional A product of Humana Press characterization of every mammalian gene and the deciphering of their molecular interaction network has become the next major challenge, and the mouse genome provides a convenient and vital model for these studies. In Gene Knockout Protocols, Printed book Second Edition, distinguished contributors with extensive experience in the gene targeting Hardcover and mouse genetics fields reveal a comprehensive collection of step-by-step laboratory ▶ 164,99 € | £145.00 | $199.00 protocols. Emphasizing the many new mutagenesis techniques developed over the last ▶ *176,54 € (D) | 181,49 € (A) | CHF 194.50 seven years, the volume covers topics such as gene modification in ES cells, stem cell manipulation, the generation of genetically engineered mice, and mutant phenotype analysis. Written in the highly successful Methods in Molecular Biology™ series format, eBook chapters contain brief introductions to their respective subjects, lists of the necessary Available from your bookstore or materials and reagents, readily reproducible protocols, and notes on troubleshooting and ▶ springer.com/shop avoiding known pitfalls. Fully updated and authoritative, Gene Knockout Protocols, Second Edition provides a MyCopy unique collection of expert bench protocols certain to be a valuable resource for those just Printed eBook for just entering the field of gene targeting but also for all genetic researchers today. ▶ € | $ 24.99 ▶ springer.com/mycopy Order online at springer.com ▶ or for the Americas call (toll free) 1-800-SPRINGER ▶ or email us at: [email protected]. ▶ For outside the Americas call +49 (0) 6221-345-4301 ▶ or email us at: [email protected]. The first € price and the £ and $ price are net prices, subject to local VAT. Prices indicated with * include VAT for books; the €(D) includes 7% for Germany, the €(A) includes 10% for Austria. Prices indicated with ** include VAT for electronic products; 19% for Germany, 20% for Austria. All prices exclusive of carriage charges. Prices and other details are subject to change without notice. All errors and omissions excepted..