Appendix 5.3 MON 810 literature review – List of all hits (June 2016-May 2017) -Web of ScienceTM Core Collection database 12/8/2016 Web of Science [v.5.23] ­ Export Transfer Service Web of Science™ Page 1 (Records 1 ­­ 50) [ 1 ] Record 1 of 50 Title: Ground beetle acquisition of Cry1Ab from plant­ and residue­based food webs Author(s): Andow, DA (Andow, D. A.); Zwahlen, C (Zwahlen, C.) Source: BIOLOGICAL CONTROL Volume: 103 Pages: 204­209 DOI: 10.1016/j.biocontrol.2016.09.009 Published: DEC 2016 Abstract: Ground beetles are significant predators in agricultural habitats. While many studies have characterized effects of Bt maize on various carabid species, few have examined the potential acquisition of Cry toxins from live plants versus plant residue. In this study, we examined how live Bt maize and Bt maize residue affect acquisition of Cry1Ab in six species. Adult beetles were collected live from fields with either current­year Bt maize, one­year­old Bt maize residue, two­year­old Bt maize residue, or fields without any Bt crops or residue for the past two years, and specimens were analyzed using ELISA. Observed Cry1Ab concentrations in the beetles were similar to that reported in previously published studies. Only one specimen of Cyclotrachelus iowensis acquired Cry1Ab from two­year­old maize residue. Three species acquired Cry1Ab from fields with either live plants or plant residue (Cyclotrachelus iowensis, Poecilus lucublandus, Poecilus chalcites), implying participation in both live­plant and residue­based food webs. Two species acquired toxin from fields with live plants, but not from fields with residue (Bembidion quadrimaculatum, Elaphropus incurvus), suggesting participation only in live plant­based food webs. One species did not acquire Cry1Ab from either live­plant or residue (Scarites quadriceps), suggesting that its food sources might not contain significant amounts of Cry1Ab. These results revealed significant differentiation among carabid species in their associations with live­plant and residue­based food webs in agricultural fields. (C) 2016 Elsevier Inc. All rights reserved. Accession Number: WOS:000386468300024 ISSN: 1049­9644 eISSN: 1090­2112 Record 2 of 50 Title: Movement and survival of Busseola fusca (Lepidoptera: Noctuidae) larvae within maize plantings with different ratios of non­Bt and Bt seed Author(s): Erasmus, A (Erasmus, Annemie); Marais, J (Marais, Jaco); Van den Berg, J (Van den Berg, Johnnie) Source: PEST MANAGEMENT SCIENCE Volume: 72 Issue: 12 Pages: 2287­2294 DOI: 10.1002/ps.4273 Published: DEC 2016 Abstract: BACKGROUNDProducts of plant biotechnology, for example genetically modified Bt maize, provide useful tools for pest management. The benefits provided by insect­resistant plants are, however, threatened by the evolution of resistance by target pest species. The high­dose/refuge insect resistance management strategy (IRM) as well as seed mixtures are globally used as IRM strategies. Busseola fusca (Lepidoptera: Noctuidae), the target stem borer of Bt maize in Africa, evolved resistance to Bt maize expressing Cry1Ab protein in South Africa. Owing to high larval mobility and subsequent sublethal exposure of larvae moving between non­Bt and Bt plants, more rapid resistance evolution has been proposed as a possibility with deployment of seed mixture strategies. RESULTSLaboratory and field studies were conducted to study B. fusca larval mobility. In the laboratory, different scenarios of B. fusca larval movement between single­ gene (Cry1Ab) and stacked­trait (Cry1A.105 and Cry2Ab2) Bt maize were studied. Data on larval survival and mass over time indicated that Cry proteins do not kill larvae above certain developmental stages. A 2 year field study with the single gene and the stacked event was conducted using seed mixtures containing 5, 10, 15 and 20% non­Bt seed as well as a control treatment (non­Bt seed only). CONCLUSIONLarval movement continued for 5 weeks and resulted in a significant incidence of Bt and non­Bt damaged plants, indicating that the movement behaviour of B. fusca is of such a nature that seed mixtures as an IRM strategy may not be effective to delay resistance evolution. (c) 2016 Society of Chemical Industry Accession Number: WOS:000386927800011 PubMed ID: 26990107 ISSN: 1526­498X eISSN: 1526­4998 Record 3 of 50 Title: Frequency of Cry1F resistance alleles in Spodoptera frugiperda (Lepidoptera: Noctuidae) in Brazil Author(s): Farias, JR (Farias, Juliano R.); Andow, DA (Andow, David A.); Horikoshi, RJ (Horikoshi, Renato J.); Bernardi, D (Bernardi, Daniel); Ribeiro, RD (Ribeiro, Rebeca da S.); do Nascimento, ARB (do Nascimento, Antonio R. B.); dos Santos, AC (dos Santos, Antonio C.); Omoto, C (Omoto, Celso) Source: PEST MANAGEMENT SCIENCE Volume: 72 Issue: 12 Pages: 2295­2302 DOI: 10.1002/ps.4274 Published: DEC 2016 Abstract: BACKGROUNDThe frequency of resistance alleles is a major factor influencing the rate of resistance evolution. Here, we adapted the F­2 screen procedure for Spodoptera frugiperda (J. E. Smith) with a discriminating concentration assay, and extended associated statistical methods to estimate the frequency of resistance to Cry1F protein in S. frugiperda in Brazil when resistance was not rare. RESULTSWe show that F­2 screen is efficient even when the resistance frequency is 0.250. It was possible to screen 517 isoparental lines from 12 populations sampled in five states of Brazil during the first half of 2012. Western Bahia had the highest allele frequency of Cry1F resistance, 0.192, with a 95% confidence interval (CI) between 0.163 and 0.220. All other states had a similar and lower frequency varying from 0.042 in Parana to 0.080 in Mato Grosso do Sul. CONCLUSIONThe high frequency in western Bahia may be related to year­round availability of maize, the high population density of S. frugiperda, the lack of refuges and the high adoption rate of Cry1F maize. Cry1F resistance alleles were not rare and occurred at frequencies that have already compromised the useful life of TC1507 maize in western Bahia. (c) 2016 Society of Chemical Industry Accession Number: WOS:000386927800012 PubMed ID: 26990201 ISSN: 1526­498X eISSN: 1526­4998 Record 4 of 50 Title: Impedimetric immunosensors for the detection of Cry1Ab protein from genetically modified maize seeds Author(s): Freitas, M (Freitas, Maria); Correr, W (Correr, Wagner); Cancino­Bernardi, J (Cancino­Bernardi, Juliana); Barroso, MF (Fatima Barroso, M.); Delerue­Matos, C (Delerue­Matos, Cristina); Zucolotto, V (Zucolotto, Valtencir) Source: SENSORS AND ACTUATORS B­CHEMICAL Volume: 237 Pages: 702­709 DOI: 10.1016/j.snb.2016.06.149 Published: DEC 2016 Abstract: Regardless the controversies surrounding genetically modified organisms (GMO), their cultivation is constantly increasing and in according to the EU legislation, labeling is mandatory for products containing EU­authorized­GMO higher than 0.9%. Thereby, new analytical strategies for rapid and effective detection of GMO on foodstuffs are required. In this work, an electrochemical immunosensor for effective determination of Cry1Ab protein from MON810 transgenic maize (EU­authorized­ GMO) is described. The immunosensor was developed onto indium tin oxide (ITO) electrodes modified by 3­aminopropyltrimethoxysilane (APTES) monolayer to covalently immobilize Anti­Cry1Ab polyclonal antibodies. The protein interaction with the polyclonal antibody (PAb) recognition platform was directly monitored and measured by cyclic voltammetry and electrochemical impedance spectroscopy using commercially Cry1Ab protein. After the analytical features optimization a linear response from 1 to 10 ng mL(­1), a limit of detection (LOD) of 0.37 ng mL(­1) and a limit of quantification (LOQ) of 1.23 ng mL(­1) ­ which provided accurate results (RSD <7.5%) ­ were achieved. The immunosensor allowed a simple and fast detection of Cry1Ab protein extracted from maize seeds with different GM maize mass percentages (0%, 0.5%, 1%, 2.5% and 5%). To crosscheck the detection of Cry1Ab protein, an enzyme­linked immunosorbent assay (ELISA) was used. The results indicate that the immunosensor is suitable for the transgenic protein Cry1Ab detection in GM maize representing a successfully tool to verify the compliance of the EU regulations. (C) 2016 Elsevier B.V. All rights reserved. Accession Number: WOS:000384010500085 Author Identifiers: Author ResearcherID Number ORCID Number Sao Carlos Institute of Physics, IFSC/USP M­2664­2016 ISSN: 0925­4005 Record 5 of 50 Title: People's reliance on the affect heuristic may result in a biased perception of gene technology Author(s): Siegrist, M (Siegrist, Michael); Sutterlin, B (Sutterlin, Bernadette) http://apps.webofknowledge.com/OutboundService.do?action=go&displayCitedRefs=true&displayTimesCited=true&displayUsageInfo=true&viewType=s… 1/12 12/8/2016 Web of Science [v.5.23] ­ Export Transfer Service Source: FOOD QUALITY AND PREFERENCE Volume: 54 Pages: 137­140 DOI: 10.1016/j.foodqual.2016.07.012 Published: DEC 2016 Abstract: In an experiment, we demonstrated that the same outcome of a new corn variety was evaluated less positively if it was from gene technology (GT) compared with conventional breeding technology (CT). The participants (N = 205) were randomly assigned to one of two experimental conditions­GT or CT. In the first step, the participants' affect associated with GT or CT plants was measured. Then, the participants read a hypothetical scenario about a new genetically modified (GT condition) or conventionally produced (CT condition) corn variety that was more resistant to the corn borer. They were presented with a matrix in which 1976 black (50%) and white (50%) cells were randomly scattered. The participants were informed that each cell represented a test plot and that the corn plants in the white test plots were not damaged by the corn borer. The participants were then asked to estimate the percentage of the plots infested by the corn borer and how effective they perceived the new corn variety was in resisting the pest. The results indicated that the participants assigned to the GT condition perceived the new corn variety as significantly less effective compared with the participants assigned to the CT condition.