Anti-Phospholipase A2 (iPLA2) (C-terminal region) produced in rabbit, affinity isolated antibody Product Number SAB4200130 Product Description iPLA2 group VIA comprises at least 5 alternatively Anti-Phospholipase A2 (iPLA2) (C-terminal region) is spliced isoforms. Isoforms LH-iPLA2 (90 kDa), and produced in rabbit using as the immunogen a synthetic SH-iPLA2 (85 kDa) iPLA2 have been implicated in peptide corresponding to a sequence at the C-terminal phospholipid remodeling, nitric oxide-induced or of human iPLA2 (GeneID 8398), conjugated to KLH. vasopressin-induced arachidonic acid release, and in The corresponding sequence is highly conserved in leukotriene and prostaglandin production. Mutations in mouse iPLA2 (83% identity) and in rat iPLA2 (72% the PLA2G6 gene are the cause of two childhood identity). The antibody is affinity-purified using the neurologic disorders, neurodegeneration with brain iron immunizing peptide immobilized on agarose. accumulation (NBIA) and infantile neuroaxonal 4,5 dystrophy 1 (INAD1). Recent evidence suggests that Anti-Phospholipase A2 (iPLA2) (C-terminal region), both cPLA2 and iPLA2 may play a central role in specifically recognizes human and rat iPLA2. The memory deficits at early stages of AD and in the AD antibody can be used in several immunochemical neurodegenerative process.6 techniques including immunoblotting (85 kDa human iPLA2, and 95 kDa rat iPLA2). Detection of the iPLA2 Reagent bands by immunoblotting is specifically inhibited by the Supplied as a solution in 0.01 M phosphate buffered iPLA2 immunizing peptide. saline, pH 7.4, containing 15 mM sodium azide. 2+ Ca -independent phospholipase A2 (iPLA2, also known Antibody concentration: 1.5 mg/mL as PLA2G6, INAD1, PARK14, PNPLA9) is a member of the PLA2 superfamily that catalyzes the cleavage of Precautions and Disclaimer fatty acids from the sn-2 position of phospholipids.1,2 For R&D use only. Not for drug, household, or other PLA2 isoenzymes vary in their cellular localizations, uses. Please consult the Safety Data Sheet for Ca2+ sensitivities, and substrate specificities. They information regarding hazards and safe handling share the ability to catalyze the synthesis of precursors practices. of proinflammatory mediators such as prostaglandins and leukotrienes through the release of arachidonic Storage/Stability acid (AA) from membrane phospholipids. Store at −20 C. For continuous use, the product may be stored at 2−8 C for up to one month. For extended PLA2s play crucial roles in several cellular processes, storage, freeze at −20 C in working aliquots. Repeated including intracellular membrane trafficking, freezing and thawing, or storage in “frost-free” freezers, differentiation, proliferation, and apoptosis. They are is not recommended. If slight turbidity occurs upon thought to play a role in oxidative and inflammatory prolonged storage, clarify the solution by centrifugation. responses in cerebral ischemia, Alzheimer’s disease Discard working dilutions if not used within 12 hours. (AD), and neuronal injury.3 Product Profile Immunoblotting: a working antibody concentration of 1-2 g/mL is recommended using extract of HEK-293T cells overexpressing human iPLA2, and 1-2 g/mL using rat kidney extract (S1 fraction). Note: In order to obtain best results in various techniques and preparations, it is recommended to determine optimal working dilutions by titration. References 1. Balsinde, J., and Balboa, M.A., Cell. Signal., 17, 4. Morgan, N.V., et al., Nat. Genet., 38, 752-754 1052-1062 (2005). (2006). 2. Pontus, K.A., et al., Eur. J. Biochem., 262, 575-585 5. Gregory, A., et al., Neurol., 71, 1402-1409 (2008). (1999). 6. Schaeffer, E.L., and Gattaz, W.F., 3. Sun, G.Y., et al., J. Lipid Res., 45, 205-213 (2004). Psychopharmacol., 198, 1-27 (2008). VS,ER,KAA,PHC,MAM 07/19-1 2019 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich Co. LLC, registered in the US and other countries. Sigma brand products are sold through Sigma-Aldrich, Inc. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see product information on the Sigma-Aldrich website at www.sigmaaldrich.com and/or on the reverse side of the invoice or packing slip. .