16S Metagenomics Report Sample: S203 Report Date: 09/08/2017 21:47:06 © 2017 Illumina, Inc. All rights reserved. Analysis software version: 2.6.2.3 16S Metagenomics Report Sample Information Sample ID: S203 Sample Name: S203 Run Folder: D:\Illumina\MiSeqAnalysis\170907_M03021_0057_000000000-BB9CG Taxonomy File: gg_13_5_species_32bp.dat Sequencing Statistics Total Reads Reads Passing % Reads Passing Quality Filtering Quality Filtering 16,985 16,595 97.7 % Classification Rate Summary Taxonomic Level Reads Classified % Total Reads Classified to Taxonomic Level to Taxonomic Level Kingdom 1,060 6.39 % Phylum 1,058 6.38 % Class 1,052 6.34 % Order 1,048 6.32 % Family 1,036 6.24 % Genus 1,029 6.20 % Species 866 5.22 % © 2017 Illumina, Inc. All rights reserved. Page 2 Analysis software version: 2.6.2.3 16S Metagenomics Report Classification Results by Taxonomic Level Tables show the highest 8 taxonomic classifications at each level. Pie charts show all classifications above 3.5% abundance. Top Kingdom Classification Results Classification Number of Reads % Total Reads Unclassified at Kingdom level 15,535 93.61 % Viruses 611 3.68 % Bacteria 448 2.70 % Archaea 1 0.01 % Note: The "Other" category in this pie chart is the sum of all classifications with less than 3.5% abundance. © 2017 Illumina, Inc. All rights reserved. Page 3 Analysis software version: 2.6.2.3 16S Metagenomics Report Top Phylum Classification Results Classification Number of Reads % Total Reads Unclassified at Phylum level 15,537 93.62 % DNA 611 3.68 % Firmicutes 209 1.26 % Proteobacteria 184 1.11 % Bacteroidetes 27 0.16 % Actinobacteria 10 0.06 % Thermi 7 0.04 % Chloroflexi 4 0.02 % Total Phylum-level Taxonomic Categories Identified: 12. This table shows the top 8 of 12 classifications. Note: The "Other" category in this pie chart is the sum of all classifications with less than 3.5% abundance. © 2017 Illumina, Inc. All rights reserved. Page 4 Analysis software version: 2.6.2.3 16S Metagenomics Report Top Class Classification Results Classification Number of Reads % Total Reads Unclassified at Class level 15,543 93.66 % Group II 611 3.68 % Bacilli 134 0.81 % Gammaproteobacteria 120 0.72 % Clostridia 75 0.45 % Alphaproteobacteria 31 0.19 % Betaproteobacteria 29 0.17 % Bacteroidia 21 0.13 % Total Class-level Taxonomic Categories Identified: 18. This table shows the top 8 of 18 classifications. Note: The "Other" category in this pie chart is the sum of all classifications with less than 3.5% abundance. © 2017 Illumina, Inc. All rights reserved. Page 5 Analysis software version: 2.6.2.3 16S Metagenomics Report Top Order Classification Results Classification Number of Reads % Total Reads Unclassified at Order level 15,547 93.68 % (Microviridae) 611 3.68 % Bacillales 105 0.63 % Clostridiales 72 0.43 % Pseudomonadales 59 0.36 % Enterobacteriales 46 0.28 % Burkholderiales 28 0.17 % Lactobacillales 28 0.17 % Total Order-level Taxonomic Categories Identified: 30. This table shows the top 8 of 30 classifications. Note: Category names appearing in parentheses are due to incomplete entries in the taxonomic database. They occur when a lower level category is specified, but the name for this higher level category is empty. Note: The "Other" category in this pie chart is the sum of all classifications with less than 3.5% abundance. © 2017 Illumina, Inc. All rights reserved. Page 6 Analysis software version: 2.6.2.3 16S Metagenomics Report Top Family Classification Results Classification Number of Reads % Total Reads Unclassified at Family level 15,559 93.76 % Microviridae 611 3.68 % Staphylococcaceae 50 0.30 % Enterobacteriaceae 46 0.28 % Planococcaceae 41 0.25 % Moraxellaceae 31 0.19 % Clostridiaceae 29 0.17 % Peptostreptococcaceae 29 0.17 % Total Family-level Taxonomic Categories Identified: 49. This table shows the top 8 of 49 classifications. Note: The "Other" category in this pie chart is the sum of all classifications with less than 3.5% abundance. © 2017 Illumina, Inc. All rights reserved. Page 7 Analysis software version: 2.6.2.3 16S Metagenomics Report Top Genus Classification Results Classification Number of Reads % Total Reads Unclassified at Genus level 15,566 93.80 % Microvirus 611 3.68 % Staphylococcus 31 0.19 % Escherichia 29 0.17 % Pseudomonas 28 0.17 % Acinetobacter 27 0.16 % Clostridium 25 0.15 % Tepidibacter 21 0.13 % Total Genus-level Taxonomic Categories Identified: 77. This table shows the top 8 of 77 classifications. Note: The "Other" category in this pie chart is the sum of all classifications with less than 3.5% abundance. © 2017 Illumina, Inc. All rights reserved. Page 8 Analysis software version: 2.6.2.3 16S Metagenomics Report Top Species Classification Results Classification Number of Reads % Total Reads Unclassified at Species level 15,729 94.78 % Microvirus Enterobacteria phage PhiX174 611 3.68 % Ralstonia pickettii 16 0.10 % Sporosarcina pasteurii 15 0.09 % Dysgonomonas wimpennyi 13 0.08 % Viridibacillus arvi 13 0.08 % Clostridium haemolyticum 12 0.07 % Macrococcus caseolyticus 12 0.07 % Total Species-level Taxonomic Categories Identified: 77. This table shows the top 8 of 77 classifications. Note: The "Other" category in this pie chart is the sum of all classifications with less than 3.5% abundance. © 2017 Illumina, Inc. All rights reserved. Page 9 Analysis software version: 2.6.2.3.