For Research Use Only. Not For Use In Diagnostic Procedures. For Research Use Only. Not For Use In Diagnostic Procedures. Terminal Deoxynucleotidyl Transferase (TdT) Enzyme Terminal Deoxynucleotidyl Transferase (TdT) Enzyme Catalog #: 9510-01K-EB Catalog #: 9510-01K-EB Contents: 9510-01K-01 TdT Enzyme Size: 1000 Units Contents: 9510-01K-01 TdT Enzyme Size: 1000 Units 4817-60-02 10X TdT Labeling Buffer 20 mL 4817-60-02 10X TdT Labeling Buffer 20 mL Description: Terminal deoxynucleotidyl transferase (TdT) is a template independent Description: Terminal deoxynucleotidyl transferase (TdT) is a template independent polymerase that catalyzes the addition of deoxynucleotides to the 3’ hydroxyl terminus of polymerase that catalyzes the addition of deoxynucleotides to the 3’ hydroxyl terminus of DNA. TdT is the enzyme of choice for R&D Systems’ TACS™ In Situ Apoptosis Detection DNA. TdT is the enzyme of choice for R&D Systems’ TACS™ In Situ Apoptosis Detection Kits which utilize TdT to incorporate biotinylated nucleotides into the fragmented DNA Kits which utilize TdT to incorporate biotinylated nucleotides into the fragmented DNA associated with apoptosis. The specificity of TdT for protruding, recessed, blunt ended associated with apoptosis. The specificity of TdT for protruding, recessed, blunt ended double stranded DNA or for single stranded DNA is cation dependent. double stranded DNA or for single stranded DNA is cation dependent. Source: Purified from E. coli containing a recombinant plasmid harboring the bovine TdT Source: Purified from E. coli containing a recombinant plasmid harboring the bovine TdT gene. gene. Unit Activity: 15 units/μL Unit Activity: 15 units/μL Unit Definition: One Unit of enzyme catalyzes the incorporation of 1 nmole of dATP into Unit Definition: One Unit of enzyme catalyzes the incorporation of 1 nmole of dATP into acid-precipitable material in one hour at 37° C under standard assay conditions. acid-precipitable material in one hour at 37° C under standard assay conditions. Substrate Specificity: Protruding, recessed, or blunt-ended double stranded DNA, or Substrate Specificity: Protruding, recessed, or blunt-ended double stranded DNA, or single stranded DNA with a free 3’-hydroxyl terminus. single stranded DNA with a free 3’-hydroxyl terminus. Assay Conditions: 20 mM potassium cacodylate, 25 mM Tris HCl (pH 7.2), 8 mM MgCl2, Assay Conditions: 20 mM potassium cacodylate, 25 mM Tris HCl (pH 7.2), 8 mM MgCl2, μ 3 μ μ 3 μ 0.33 mM ZnSO4, 0.2 mM dATP, 42 pmol oligo d(A)18, and 0.5 Ci H dATP (1 - 0.4 M) in a 0.33 mM ZnSO4, 0.2 mM dATP, 42 pmol oligo d(A)18, and 0.5 Ci H dATP (1 - 0.4 M) in a 50 μL total reaction volume. 50 μL total reaction volume. β β Storage Buffer: 60 mM KPO4, 150 mM KCl, 1 mM -mercaptoethanol, 0.5% Triton X-100, Storage Buffer: 60 mM KPO4, 150 mM KCl, 1 mM -mercaptoethanol, 0.5% Triton X-100, 50% (v/v) glycerol at a pH of 7.2. 50% (v/v) glycerol at a pH of 7.2. Storage Conditions: The enzyme is stable for at least 1 year when stored at -20° C in a Storage Conditions: The enzyme is stable for at least 1 year when stored at -20° C in a manual defrost freezer. For long term storage, aliquot and store at -80° C. Avoid repeated manual defrost freezer. For long term storage, aliquot and store at -80° C. Avoid repeated freeze-thaw cycles. The labeling buffer may be stored at 2 - 8° C. freeze-thaw cycles. The labeling buffer may be stored at 2 - 8° C. TACS is a trademark of Trevigen, Inc. 10/07 TACS is a trademark of Trevigen, Inc. 10/07 R&D Systems, Inc. R&D Systems, Inc. 614 McKinley Place NE, Minneapolis, MN 55413 USA 614 McKinley Place NE, Minneapolis, MN 55413 USA Tel: 1-800-343-7475 • 612-379-2956 • Fax: 612-656-4400 Tel: 1-800-343-7475 • 612-379-2956 • Fax: 612-656-4400 e-mail: [email protected] • www.RnDSystems.com e-mail: [email protected] • www.RnDSystems.com Terminal Deoxynucleotidyl Terminal Deoxynucleotidyl Transferase (TdT) Enzyme Transferase (TdT) Enzyme Catalog #: 9510-01K-EB Catalog #: 9510-01K-EB Storage: -20° C Storage: -20° C R&D Systems R&D Systems 1-800-343-7475 1-800-343-7475.