SUPPLEMENTAL MATERIAL Online OnlY Supplemental material miRNA expression profiling of cerebrospinal fluid in patients with cerebral aneurysmal subarachnoid hemorrhage S. S. Stylli et al. http://thejns.org/doi/abs/10.3171/2016.1.JNS151454 DisClaimer The Journal of Neurosurgery acknowledges that the following section is published verbatim as submitted by the authors and did not go through either the Journal’s peer-review or editing process. ©AANS, 2016 J Neurosurg Supplementary Table 1 nSolver Differential Expression Analysis Comparison Groups Comparison 1 No SAH SAH / No vasospasm (combined) CSF0012, CSF0018, CSF0030, CSF0034 CSF0020, CSF0023, CSF0024, CSF0025, CSF0038, CSF0039, CSF0040, CSF0041, CSF0042 Comparison 2 No SAH SAH / No vasospasm (Sample Day 1) CSF0012, CSF0018, CSF0030, CSF0034 CSF0020, CSF0023, CSF0024, CSF0027, CSF0041, CSF0038 Comparison 3 No SAH SAH / Vasospasm (combined) CSF0012, CSF0018, CSF0030, CSF0034 CSF0 022, CSF0027, CSF0032, CSF0033, CSF0036, CSF0044, CSF0045, CSF0048, CSF0052, CSF0053 Comparison 4 No SAH SAH / Vasospasm (Sample Day 1) CSF0012, CSF0018, CSF0030, CSF0034 CSF0027, CSF0032, CSF0036, CSF0044, CSF0052 Comparison 5 No SAH SAH / Vasospasm (post Sample Day 1) CSF0012, CSF0018, CSF0030, CSF0034 CSF0022, CSF0033, CSF0045, CSF0048, CSF0053 Comparison 6 SAH / No vasospasm (combined) SAH / Vasospasm (combined) CSF0020, CSF0023, CSF0024, CSF0025, CSF0022, CSF0027, CSF0032, CSF0033, CSF0036, CSF0038, CSF0039, CSF0040, CSF0041, CSF0044, CSF0045, CSF0048, CSF0052, CSF0053 CSF0042 Comparison 7 SAH / No vasospasm (Sample Day 1) SAH / Vasospasm (Sample Day 1) CSF0020, CSF0023, CSF0024, CSF0038, CSF0027, CSF0032, CSF0036, CSF0044, CSF0052 CSF0041 Comparison 8 SAH / No vasospasm (post Sample Day 1) SAH / vasospasm (post Sample Day 1) CSF0025, CSF0038, CSF0039, CSF0040, CSF0022, CSF0033, CSF0045, CSF0048, CSF0053 CSF0042 Supplementary Table 2 miRNA showing statistically significant fold level changes as determined by nSolver Analysis Software v2.6 Comparison 1 No SAH versus SAH / No vasospasm (combined) p-value Control Mean Case Mean Fold Change hsa-miR-451a* 0.0002 17.53 1262.2 72.01 hsa-miR-152* 0.0008 22.1 12.32 -1.79 hsa-miR-223-3p* 0.0009 9.81 28.94 2.95 hsa-miR-599* 0.0014 13.37 8.75 -1.53 hsa-miR-1224-3p* 0.0023 18.37 11.67 -1.57 hsa-miR-1301* 0.0036 13.73 8.24 -1.67 hsa-miR-548m* 0.0038 21.35 15.01 -1.42 hsa-miR-204-5p* 0.0045 13.43 42.28 3.15 hsa-miR-626* 0.0046 29.37 15.64 -1.88 hsa-miR-3180-5p* 0.0073 14.34 9.46 -1.52 hsa-miR-421* 0.0074 17.2 11.71 -1.47 hsa-miR-606* 0.0091 18.57 13 -1.43 hsa-miR-26a-5p* 0.0092 20.67 13.47 -1.53 hsa-miR-376c* 0.0097 17.1 11.62 -1.47 hsa-miR-3127-5p 0.0113 13.58 8.51 -1.6 hsa-miR-215 0.0179 30.85 19.23 -1.6 hsa-miR-1244 0.0254 34.11 23.91 -1.43 hsa-miR-580 0.0268 18.78 12.49 -1.5 hsa-miR-652-3p 0.027 12.13 8.24 -1.47 hsa-miR-452-5p 0.0291 12.7 6.85 -1.85 hsa-miR-521 0.0302 12.65 8.63 -1.47 hsa-miR-346 0.0359 20.2 11.63 -1.74 hsa-miR-510 0.0396 13.21 8.86 -1.49 hsa-miR-615-3p 0.0431 14.81 7.51 -1.97 hsa-miR-210 0.0432 16.03 10.56 -1.52 hsa-miR-485-5p 0.0454 18.94 12.16 -1.56 hsa-miR-144-3p 0.0465 25.77 66.32 2.57 Comparison 2 No SAH versus SAH / No vasospasm (Sample Day 1) p-value Control Mean Case Mean Fold Change hsa-miR-301a-3p* 0.0024 23.15 13.44 -1.72 hsa-miR-378d* 0.0087 18.44 7.94 -2.32 hsa-miR-596 0.0105 18.09 12.11 -1.49 hsa-miR-197-3p 0.0178 17.24 9.98 -1.73 hsa-miR-326 0.0207 10.29 4.43 -2.32 hsa-miR-576-3p 0.0258 15.07 24.64 1.64 hsa-miR-301b 0.0294 18.47 11 -1.68 hsa-miR-548y 0.0296 20.17 10.28 -1.96 hsa-miR-148b-3p 0.0324 18.65 12.2 -1.53 hsa-miR-890 0.0344 21.24 28.93 1.36 hsa-miR-370 0.0367 13.18 19.06 1.45 hsa-miR-486-3p 0.0369 20.56 12.88 -1.6 hsa-miR-4647 0.0405 19.59 12.51 -1.57 hsa-miR-3690 0.0443 21.96 13.52 -1.62 hsa-miR-1291 0.049 14.67 9.46 -1.55 hsa-miR-491-5p 0.0496 20.52 11.24 -1.83 Comparison 3 No SAH versus SAH / vasospasm (combined) p-value Control Mean Case Mean Fold Change hsa-miR-451a* 0.0005 19.26 1145.97 59.5 hsa-miR-937* 0.0006 20.27 11.19 -1.81 hsa-miR-519b-3p* 0.001 14.66 8.68 -1.69 hsa-miR-1224-3p* 0.0013 20.18 9.69 -2.08 hsa-miR-548m* 0.0014 23.46 14.3 -1.64 hsa-miR-1244* 0.0015 37.48 20.01 -1.87 hsa-miR-1301* 0.0018 15.08 8.78 -1.72 hsa-miR-2117* 0.0021 20.8 15.98 -1.3 hsa-miR-489* 0.0024 34.45 21.69 -1.59 hsa-miR-204-5p* 0.0034 14.75 41.16 2.79 hsa-miR-514b-3p* 0.0034 16.87 8.49 -1.99 hsa-miR-223-3p* 0.004 10.77 59.75 5.55 hsa-miR-521* 0.0044 13.9 7.99 -1.74 hsa-miR-624-3p* 0.0048 17.53 11.74 -1.49 hsa-miR-708-5p* 0.0052 11.86 6.75 -1.76 hsa-miR-508-3p* 0.0062 16.86 9.12 -1.85 hsa-miR-345-5p* 0.0073 17.63 9.39 -1.88 hsa-miR-137* 0.0077 18.83 10.74 -1.75 hsa-miR-599* 0.0077 14.69 7.71 -1.9 hsa-miR-4521* 0.0079 15.26 7.96 -1.92 hsa-miR-337-5p* 0.0089 21.18 17.09 -1.24 hsa-miR-320e* 0.0094 20.34 63.34 3.11 hsa-miR-346* 0.0095 22.19 13.43 -1.65 hsa-miR-152 0.0102 24.28 15.27 -1.59 hsa-miR-376c 0.011 18.79 14.85 -1.27 hsa-miR-198 0.0116 22.96 12.99 -1.77 hsa-miR-655 0.0118 18.89 8.15 -2.32 hsa-miR-580 0.0123 20.64 12.04 -1.71 hsa-miR-764 0.013 17.37 10.66 -1.63 hsa-miR-744-5p 0.0141 19.2 11.35 -1.69 hsa-miR-302a-3p 0.0177 13.69 10.37 -1.32 hsa-miR-548z 0.0189 29.2 19.87 -1.47 hsa-miR-485-5p 0.0196 20.81 11.99 -1.74 hsa-miR-548l 0.0201 15.28 11 -1.39 hsa-miR-548k 0.0202 24.2 15.79 -1.53 hsa-miR-328 0.0224 11.96 8.28 -1.44 hsa-miR-891a 0.0249 31.24 22.79 -1.37 hsa-miR-215 0.0262 33.89 24.02 -1.41 hsa-miR-4458 0.027 23.38 13.61 -1.72 hsa-miR-3147 0.0281 19.65 10.18 -1.93 hsa-miR-1277-3p 0.0284 21.41 13.34 -1.6 hsa-miR-26a-5p 0.0289 22.71 14.74 -1.54 hsa-miR-525-3p 0.0305 12.1 5.78 -2.1 hsa-miR-452-5p 0.0317 13.95 8.13 -1.72 hsa-miR-548c-5p 0.0324 21.73 14.63 -1.49 hsa-miR-765 0.0325 13.19 7.87 -1.68 hsa-miR-652-3p 0.0326 13.32 8.2 -1.62 hsa-miR-648 0.0332 15.93 8.43 -1.89 hsa-miR-615-3p 0.0339 16.27 9.39 -1.73 hsa-miR-649 0.0351 16.92 7.96 -2.13 hsa-miR-518e-3p 0.0356 9.78 6.06 -1.61 hsa-miR-519c-3p 0.0357 23.89 16.61 -1.44 hsa-miR-510 0.0365 14.51 9.28 -1.56 hsa-miR-516a-5p 0.0387 10.85 8.18 -1.33 hsa-miR-520h 0.0392 19.99 13.05 -1.53 hsa-miR-15a-5p 0.0398 19.36 27.22 1.41 hsa-miR-1281 0.0399 16.19 11.78 -1.37 hsa-miR-607 0.0402 16.82 8.06 -2.09 hsa-miR-133b 0.0418 15.77 12.3 -1.28 hsa-miR-146b-3p 0.042 12.5 8.43 -1.48 hsa-miR-421 0.0436 18.9 13.97 -1.35 hsa-miR-1303 0.0443 13.7 8.16 -1.68 hsa-miR-369-3p 0.0469 25.35 18.74 -1.35 hsa-miR-552 0.0469 15.84 10.83 -1.46 hsa-miR-596 0.0474 19.28 12.48 -1.55 hsa-miR-924 0.0475 13.91 10.51 -1.32 hsa-miR-1224-5p 0.0482 11.98 8.02 -1.49 hsa-miR-217 0.0488 39.9 26.6 -1.5 hsa-miR-320d 0.0492 12.6 8.43 -1.49 hsa-miR-626 0.0498 32.27 20.93 -1.54 hsa-miR-409-3p 0.0499 16.33 11.74 -1.39 Comparison 4 No SAH versus SAH / vasospasm (Sample Day 1) p-value Control Mean Case Mean Fold Change hsa-miR-548m* 0.0028 22.34 13.15 -1.7 hsa-miR-337-5p* 0.0056 20.17 16.68 -1.21 hsa-miR-519b-3p* 0.0078 13.96 8.06 -1.73 hsa-miR-764 0.0102 16.54 10.46 -1.58 hsa-miR-514b-3p 0.012 16.07 8.07 -1.99 hsa-miR-1301 0.019 14.36 8.91 -1.61 hsa-miR-204-5p 0.0195 14.05 45.58 3.24 hsa-miR-545-3p 0.0202 10.55 6.43 -1.64 hsa-miR-26a-5p 0.0226 21.63 15.02 -1.44 hsa-miR-3180-5p 0.0249 15.01 9.25 -1.62 hsa-miR-521 0.0272 13.24 7.02 -1.89 hsa-miR-599 0.0281 13.99 8.4 -1.67 hsa-miR-4448 0.0297 13.99 8.56 -1.63 hsa-miR-508-3p 0.0311 16.06 8.27 -1.94 hsa-miR-137 0.0346 17.93 12.2 -1.47 hsa-miR-525-3p 0.035 11.53 6.17 -1.87 hsa-miR-708-5p 0.035 11.3 5.93 -1.91 hsa-miR-23c 0.037 31.77 21.64 -1.47 hsa-miR-937 0.0408 19.31 12.05 -1.6 hsa-miR-1246 0.0442 1.12 1.03 -1.09 hsa-miR-1253 0.0442 1.12 1.03 -1.09 hsa-miR-1283 0.0442 1.12 1.03 -1.09 hsa-miR-143-3p 0.0442 1.12 1.03 -1.09 hsa-miR-1244 0.0457 35.69 23.56 -1.52 hsa-miR-451a 0.0463 18.34 722.04 39.37 hsa-miR-10b-5p 0.0483 13 6.74 -1.93 hsa-miR-146b-3p 0.0485 11.91 7.93 -1.5 hsa-miR-548q 0.0493 10.53 5.28 -1.99 hsa-miR-323a-5p 0.0498 12.9 8.27 -1.56 Comparison 5 No SAH versus SAH / vasospasm (post Sample Day 1) p-value Control Mean Case Mean Fold Change hsa-miR-489* 0.0007 33.41 17.31 -1.93 hsa-miR-548l* 0.0013 14.82 9.09 -1.63 hsa-miR-937* 0.003 19.66 9.81 -2 hsa-miR-302a-3p* 0.0038 13.28 8.22 -1.61 hsa-miR-1224-3p* 0.008 19.57 7.5 -2.61 hsa-miR-451a* 0.0093 18.68 2029.1 108.64 hsa-miR-345-5p* 0.0096 17.09 8.78 -1.95 hsa-miR-520h 0.0119 19.39 10.45 -1.86 hsa-miR-580 0.0165 20.01 10.74 -1.86 hsa-miR-320e 0.0172 19.72 82.09 4.16 hsa-miR-383 0.0177 16.03 11.4 -1.41 hsa-miR-548c-5p 0.018 21.08 12.23 -1.72 hsa-miR-1244 0.0184 36.35 16.03 -2.27 hsa-miR-1277-3p 0.0221 20.76 11 -1.89 hsa-miR-152 0.0229 23.55 13.58 -1.73 hsa-miR-1224-5p 0.0246 11.62 6.51 -1.78 hsa-miR-649 0.025 16.41 6.64 -2.47 hsa-miR-328 0.0254 11.6 7.21 -1.61 hsa-miR-2117 0.027 20.17 15.01 -1.34 hsa-miR-566 0.0275 21.66 13.9 -1.56 hsa-miR-409-3p 0.0278 15.84 9.38 -1.69 hsa-miR-4521 0.0292 14.8 5.69 -2.6 hsa-miR-378b 0.0302 21.54 13.24 -1.63 hsa-miR-508-3p 0.0318 16.36 9.49 -1.72 hsa-miR-1270 0.0322 16.51 10.81 -1.53 hsa-miR-624-3p 0.0338 17.01 9.61 -1.77 hsa-miR-496 0.0342 13.65 7.54 -1.81 hsa-miR-320d 0.0369 12.22 7.46 -1.64 hsa-miR-516a-5p 0.0381 10.52 7.03 -1.5 hsa-miR-655 0.0405 18.32 6.73 -2.72 hsa-miR-3147 0.0406 19.05 7.03 -2.71 hsa-miR-337-5p 0.0427 20.54 16.52 -1.24 hsa-miR-1301 0.0428 14.63 8.17 -1.79 hsa-miR-548m 0.0435 22.75 14.68 -1.55 hsa-miR-548k 0.0446 23.47 13.41 -1.75 hsa-miR-217 0.0447 38.7 23.66 -1.64 hsa-miR-215 0.0456 32.87 21.47 -1.53 hsa-miR-196b-5p 0.0462 16.51 8.54 -1.93 hsa-miR-708-5p 0.0462 11.51 7.25 -1.59 hsa-miR-421 0.0475 18.33 12.82 -1.43 hsa-miR-876-5p 0.0489 12.82 7.25 -1.77 hsa-miR-891a 0.0489 30.3 19.59 -1.55 hsa-miR-346 0.0492 21.52 13.28 -1.62 hsa-miR-133b 0.0493 15.3 10.44 -1.47