OriGene Technologies, Inc. OriGene Technologies GmbH 9620 Medical Center Drive, Ste 200 Schillerstr. 5 Rockville, MD 20850 32052 Herford UNITED STATES GERMANY Phone: +1-888-267-4436 Phone: +49-5221-34606-0 Fax: +1-301-340-8606 Fax: +49-5221-34606-11 [email protected] [email protected] AM05324PU-N Monoclonal Antibody to SMC2 / CAPE - Purified Alternate names: CAP-E, Chromosome-associated protein E, SMC protein 2, SMC-2, SMC2L1, Structural maintenance of chromosomes protein 2, XCAP-E homolog Quantity: 0.1 mg Concentration: 1.0 mg/ml Background: CAP-E and CAP-C (Chromosome Associated Protein-E & C) are also SMC (Structural Maintenance of Chromosome) family members that form a heterodimeric complex required for mitotic chromosome condensation to achieve proper segregation of genetic information during subsequent cell division. hCAP-C/hCAP-E is a component of a multiprotein complex called condensin that interacts with phosphorylation of Histone H3 resulting in the mitotic chromosome condensation. The distribution patterns of the two heterodimeric complexes in interphase nucleus indicate independent behaviour of the two complexes during cell cycle. These results suggest that the two distinct complexes are involved in different aspects of mitotic chromosome organization in human cells. Uniprot ID: O95347 NCBI: NP_001036015.1 GeneID: 10592 Host / Isotype: Mouse / IgG Clone: E1M Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant protein corresponding to amino acids 523-768 of Human CAP-E and Mouse myeloma cells. Genename: SMC2 Format: State: Liquid purified IgG fraction. Buffer System: PBS containing 0.08% Sodium Azide as preservative. Applications: Western Blot: 1-5 µg/ml. Positive Control/Tissue Expression: HeLa cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: Recognizes SMC2/CAPE. Species: Human. Other species not tested. Storage: The antibody can be shipped at ambient temperature. Store (in aliquots) at -20°C. Shelf life: one year from despatch. General Readings: 1. Hirano T, Kobayashi R, Hirano M. Condensins, chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosophila Barren protein. Cell. 1997 May 16;89(4):511-21. PubMed PMID: 9160743. 2. Schmiesing JA, Gregson HC, Zhou S, Yokomori K. A human condensin complex For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20130513 1 / 2 AM05324PU-N: Monoclonal Antibody to SMC2 / CAPE - Purified containing hCAP-C-hCAP-E and CNAP1, a homolog of Xenopus XCAP-D2, colocalizes with phosphorylated histone H3 during the early stage of mitotic chromosome condensation. Mol Cell Biol. 2000 Sep;20(18):6996-7006. PubMed PMID: 10958694. Pictures: Western blot analysis: AM05324PU-N CAPE antibody staining of HeLa cell lysate. For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20130513 2 / 2 Powered by TCPDF (www.tcpdf.org).