European science review № 9–10 2018 September–October Volume 1 PREMIER Vienna Publishing 2018 European Sciences review Scientific journal № 9–10 2018 (September–October) Volume 1 ISSN 2310-5577 Editor-in-chief Lucas Koenig, Austria, Doctor of Economics International editorial board Abdulkasimov Ali, Uzbekistan, Doctor of Geography Kocherbaeva Aynura Anatolevna, Kyrgyzstan, Doctor of Economics Adieva Aynura Abduzhalalovna, Kyrgyzstan, Doctor of Economics Kushaliyev Kaisar Zhalitovich, Kazakhstan, Doctor of Veterinary Medicine Arabaev Cholponkul Isaevich, Kyrgyzstan, Doctor of Law Lekerova Gulsim, Kazakhstan, Doctor of Psychology Zagir V. 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Typeset in Berling by Ziegler Buchdruckerei, Linz, Austria. Printed by Premier Publishing s.r.o., Vienna, Austria on acid-free paper. STUDY OF MUTATIONS IN THE 23 S rRNA GENE ARE ASSOCIATED WITH CLARITHROMYCIN RESISTANCE IN H.PYLORI Section 1. Biology Abdurakhimov Abrorjon Akramovich, researcher, Institute of Bioorganic Chemistry of the Academy of Sciences of Republic Uzbekistan, Tashkent E-mail: [email protected] Dalimova Dilbar Akbarovna, Senior Researcher, Center for Advanced Technologies of the Republic of Uzbekistan, Tashkent E-mail: [email protected] Turdikulova Shakhlo Utkurovna, doctor of biological science, Center for Advanced Technologies of the Republic of Uzbekistan, Tashkent E-mail: [email protected] STUDY OF MUTATIONS IN THE 23 S rRNA GENE ARE ASSOCIATED WITH CLARITHROMYCIN RESISTANCE IN H.PYLORI Abstract: Uzbekistan living in patients diagnosed with atrophic gastritis and stomach ulcers caused by H.pylori bacteria clarithromycin 23S rRNA gene that provides resistance to the domain V 2142 A/G/C, 2143 A/G muta- tions Multiplex Real-Time PCR method. 114 atrophic gastritis and gastric ulcer patients out of 109 (95,6%) caused by H.pylori bacteria and 114 samples, 19 (16,6%) caused by H.pylori 23S rRNA gene isolated from the domain V 2142 A/G/C, 2143 A/G mutations. Of them, 15 (78,9%) A2142G, 3 (15,79%), A214G and 1% (5,268) A2142C mutation was present. Keywords: H.pylori, clarithromycin, 23S rRNA, multiplex Real-time PCR, gene ureC. Helicobacter pylori is one of the most studied bacteria in eases, provides for anti-Helicobacter therapy, which, as a rule, the world today. Approximately 60% of the world’s popula- is carried out without determining the sensitivity of H. pylori tion is infected with H.pylori. For example, the infection of to antibacterial agents. the adult population of Russia ranges from 50 to 80%, and However, to increase the effectiveness of treatment of pa- in some regions it approaches 100% [1, p. 2–12; 2, p. 41]. tients with this pathology, it is necessary to take into account In infected people, H.pylori causes a disease associated with the resistance of H.pylori to antibacterial drugs. It is known asymptomatic carriage. At the same time, epidemiological that the Maastricht Agreement-5 is the main advisory docu- data obtained in various countries show that almost 100% ment on the diagnosis and treatment of diseases associated of duodenal ulcers and more than 80% of gastric ulcers are with H.pylori infection. This scientific work provides for the associated with persistence of H. pylori [1, 2–12; 2, 41]. To- empirical use of antibacterial drugs for therapy at a known day, it is proved that H.pylori infection is the most important level of H.pylori resistance and makes it possible to accurately etiopathogenetic factor not only of a peptic ulcer, but also of predict the effectiveness of therapy [3, 1–25.]. The resistance chronic gastritis associated with H.pylori in 75–92% of cases of H. pylori to antibacterial drugs is a major factor in the fail- of gastroduodenitis, stomach cancer [2, 41]. ure of treatment of Helicobacter-associated diseases. This pa- H.pylori has a wide range of genetic factors that provide it per presents data on the resistance of H.pylori strains to clar- with the ability to adapt and survive in an acidic environment, ithromycin in the region, which is associated with a decrease colonizing the mucous membrane of the stomach and duode- in the effectiveness of standard therapy regimens. num, and cause disease. It is known that in modern medicine The aim of this work is to determine the prevalence the treatment of patients with Helicobacter-associated dis- of A2142G/C and A2143G point mutations that form 3 Section 1. Biology clarithromycin resistance in Uzbekistan based on Real-Time the bacterium H. pylori was carried out. Sequencing of the polymerase chain reaction (PCR-Real-time PCR). strains of the bacterium H.pylori resistant to clarithromycin Materials and methods in the 23S rRNA gene revealed several mutations, of which In 2016–2017 in the department of Gastroenterology of 2142 A/G/C, 2143 A/G mutations were most common, and the Republican Specialized Scientific and Practical Medical the mutation 2142 A/C was not detected [6, 1–6; 7, e29694]. Center for Therapy and Medical Rehabilitation of the Minis- The sequencing of resistant