Objectives for biological microscopes CFI Plan Apochromat Lambda Series Objectives for biological microscopes CFI Plan Apochromat Lambda Series Nano Crystal Coat guarantees optimum brightness Nikon’s exclusive ultra-low refractive index coating technology “Nano Crystal Coat,” used in the manufacture High-quality materials and technology — the foundations of Nikon lens products of professional SLR camera lenses, is now employed in the new CFI Plan Apochromat λ objective series. This technology enables remarkably high transmission throughout a broad range of wavelengths, from UV to the Nikon’s extremely reliable high-tech products have Nikon draws upon experience and technologies that have near-IR region. Offering bright, sharp, high-contrast images, these lenses are perfect for multi-color incorporated the company’s cutting-edge optical and been accumulated through the production of precision optics. precision technologies since 1917. Over the past century, This enables the transfer of skills and knowledge between fluorescence live-cell imaging, particularly for fluorescent dyes with longer wavelengths that are less Nikon has researched and developed optical glass products in semiconductor equipment, camera lenses and also in the phototoxic to living specimens. Moreover, with the world’s highest level of chromatic aberration correction, combination with optical designs for cameras, microscopes, production of microscope objective lenses. In 1976, Nikon resolution and image flatness, they ensure the capture of high-quality brightfield images. Capable of semiconductor exposure equipment and others. released the CF (Chromatic aberration Free) objectives, which visualizing the minute structures and dynamics of living cells or organisms, the CFI Plan Apochromat λ series corrected chromatic aberration within the objective itself. These was followed in 1996 by the CFI60 (Chromatic supports bioscience research in its quest to reveal the mechanisms of life. aberration Free Infinity) series, which combined the benefits of the CF optics with an infinity corrected optical system. Since then, Nikon has met the needs of diverse bioscience research by manufacturing market-leading objective lenses such as the CFI Plan Apochromat VC series, CFI Apochromat TIRF series and CFI Apochromat λS series. The front lens, which is the lens element at the tip of a high- power objective, is extremely small and has a distinctive shape. A highly skilled expert must grind the lens by hand to meet the required high-precision standards and desired shape. The ground lens is then stringently and repeatedly checked using high-precision processing technology to ensure it meets Nikon’s compulsory high performance. Nikon Master Craftperson Within the Nikon organization, there are dedicated personnel with the title of Nikon Master Craftperson. They have passed rigorous tests and possess a high degree of skill and expert knowledge, specifically for the production of objective lenses. Everyday, these “masters” utilize their techniques and knowledge to deliver unrivalled glass-based optical solutions. Nano Crystal Coat is Nikon’s superlative coating technology With its origins in Nikon's semiconductor manufacturing Nano Crystal Coat eliminates ghost effects caused by red technology, Nano Crystal Coat is an anti-reflective coating light, an achievement that has taken a long time, and that assimilates ultra-fine crystallized particles of nanometer effectively reduces flare effects caused by light entering the size. With particles arranged in a spongy construction with lens at an angle. uniform spaces between them, this coarse structure enables lower refractive indices, facilitating the passage of light Incident light Reflected light Incident light Reflected light through the lens. These crystallized particles eliminate reflections inside the lens throughout the spectrum of visible light waves in ways that far exceed the limits of conven- tional anti-reflective coating systems. Lens Lens Conventional multi-layer coating Nano Crystal Coat 2 3 Stunningly sharp and clear imaging for applications from bioscience research and drug discovery to routine clinical analysis The newly developed high resolution CFI Plan Apochromat λ series objectives provide chromatic aberration correction throughout the wide wavelength range of 435 nm to 850 nm, and high transmittance up to the near IR range. They are suitable for acquiring bright, high-contrast, multi-wavelength fluorescence images, near IR images and confocal 3D images. They also capture clear and sharp brightfield images. Multi-wavelength fluorescence image using multiple dyes Three-dimensional fluorescence images Image of HeLa cells labeled with four probes: Hoechst33342 (Nuclei, blue), Venus (Mitochondria, green), Honey bee antenna mCherry (a-tubulin, orange), Alexa 750 (Nucleoli, red) DAPI: Cell nucleus FITC: Dorsal branch of the antennal nerve Rhodamine: Ventral branch of the antennal nerve Objective lens: CFI Plan Apochromat λ 100x oil Image of HeLa cells labeled with four probes: Hoechst33342 (Nuclei, blue), Venus (Mitochondria, green), mCherry (a-tubulin, orange), Alexa 700 (Nucleoli, red) Objective lens: CFI Plan Apochromat λ 20x Objective lens: CFI Plan Apochromat λ 40x Specimen courtesy of: Dr. Hiroshi Nishino and Dr. Takeharu Nagai, Research Institute for Electronic Science, Hokkaido University Objective lens: CFI Plan Apochromat λ 40x Photos courtesy of: Pathology examination image Dr. Kenta Saito, Dr. Masahiro Nakano, Dr. Kentarou Kobayashi, Dr. Takeharu Nagai, Research Institute for Electronic Science, Hokkaido University Objective lens: CFI Plan Apochromat λ 10x Membranous glomerulonephritis, HE staining Objective lens: CFI Plan Apochromat λ 20x Near-IR dye images Indocyanine green (ICG) fluorescence image BALB/c mice were administered with IRDye800CW 2-DG via tail vein injection one week of mouse auricularis blood vessels after the 4T1 breast cancer cells expressing mCherry were transplanted. Twenty-four hours post-injection, increased uptake of 2-deoxyglucose (red) in cells was observed, consistent with 4T1 breast cancer cells (blue) in the tumors, and increased sugar metabolism in the tumor cells was apparent. Photos courtesy of: Yoji Urata, MD. PhD, Department of Pathology, Kyoto City Hospital Gastric cancer, Ki-67 immunostaining ＋＝ Objective lens: CFI Plan Apochromat λ 10x Objective lens: CFI Plan Apochromat λ 20x Excitation wavelength: 785nm Photos courtesy of: Peak emission wavelength: 832nm Hirofumi Inoue M.D., Ph.D., Department of Biochemistry and Molecular Genetics, Ehime University Graduate School of Medicine, Objective lens: CFI Plan Apochromat λ 20x Ehime-Nikon Bioimaging Core Laboratory, Proteo-Medicine Research Center (ProMRes), Ehime University Photos courtesy of: Yoji Urata, MD. PhD, Department of Pathology, Kyoto City Hospital 4 5 High-resolution images with unbeatable brightness and sharpness at CFI Plan Apochromat λSeries any magnification and wavelength High transmission rates across a broad High numerical aperture for bright images spectrum range Unprecedented high resolution with a high NA (Numerical Aperture) By incorporating Nikon’s leading optical technologies and Nano can be achieved at any magnification. The CFI Plan Apochromat λ Crystal Coat, the objectives have dramatically increased transmission 100x oil objective has an incredibly high NA of 1.45, enabling the rates throughout the entire visible range, from UV to near infrared. capture of sharp, crystal clear images of minute structures. CFI Plan Apochromat λ objectives minimize the possibility of Moreover, the exceptional brightness of these lenses minimizes the damage to live cells and enable long-term imaging thanks to their use of excitation light to reduce the effects of photobleaching. ability to image by maximizing brightness with minimum excitation intensity. Vastly improved transmission ratio (CFI Plan Apochromat λ60X oil) 1 0.8 0.6 CFI Plan Apochromat λ DM Series 0.4 Transmittance 0.2 Conventional 100x oil lens 0 300 400 500 600 700 800 900 1000 Wavelength（nm） Chromatic aberration correction to near IR range λ series 100x oil lens Chromatic aberrations are corrected over a wide wavelength range, High-resolution, high-contrast images of minute structures can be acquired with high NA of 1.45. extending from 435 nm to 850 nm, so crystal clear images are Specimen: Diatoms captured during multi-wavelength imaging. By correcting the focus shift for all wavelengths, the focus point is maintained when Superior image flatness Specifications switching between dyes. More reliable intensity data for each color Image flatness is maintained across the entire field of view for all can be acquired when visualizing the structure and functions of a Fluorescence objectives from low to high magnifications because of Nikon’s W.D. Cover glass Correction Phase living specimen using multiple fluorescence proteins in confocal Model NA Brightfield Darkfield DIC Polarizing (from visible outstanding optical design technology. (mm) thickness ring contrast spectral imaging. light to NIR) Unobtrusive lens shape CFI Plan Apochromat λ2X 0.10 8.50 ― ◎ × × × ○ ◎ Searching for and confirming the observation point is difficult with CFI Plan Apochromat λ4X 0.20 20.00 ― ◎ × × × ○ ◎ conventional objectives that have a bulky lens top. There is always a CFI Plan Apochromat λ10X 0.45 4.00 0.17 ◎ △ ◎ × ○ ◎ risk that part of the lens could touch the specimen. The CFI Plan Apochromat λ series objectives have a more acute top lens design, CFI Plan Apochromat λ20X 0.75