'"------- HST 423 - Modem Science in World History Prof. Zuoyue Wang 12 March 2008 Beyond Photograph 51 The discovery of the structure of DNA raises the concern about women in male dominated fields, such as science and engineering, due to the discrimination that Rosalind "Rosy" Franklin faced at King's College while working on DNA X-ray crystallography. Not only was Franklin mistreated at King's College, but she also wasn't given the credit she deserved for her DNA knowledge and data that led to the discovery of the structure of DNA. The most imminent data that Franklin produced was Picture 51, which clearly showed that DNA was double helical, and was used by Watson and Crick without referencing it. The discrimination against Rosalind Franklin was brought to public attention when The Double Helix was published in 1968, by the sexist way author James D. Watson portrayed Franklin. The Double Helix caused many people underemphasize Franklin's scientific abilities and contribution to the discovery of the structure of DNA. For example, James Watson wrote that " ...she was incompetent in interpreting X-ray pictures. Ifonly she would learn some theory,,,l hence labeling her work as mere data gathering, and unable to synthesize scientific theories about DNA. Rosalind Franklin was not only a skillful crystallographer and data supplier, but beyond Photograph 51 she was a brilliant scientist, discovering important facts regarding the forms, components, and configuration of DNA that have helped us better understand this controversial molecule. I Watson, James D., The Double Helix (New York: Touchstone, 2001), 166. 1 Rosalind Franklin, the "incompetent" scientist at the eyes of her colleagues, was successful at making accurate conclusions about DNA's configuration, which Watson and Crick referenced for their model. Franklin was one the first to suggest that the phosphates go on the outside, and that the bases go in between the DNA strands. When Watson and Crick constructed their first DNA model, Franklin corrected the model's mistaken configuration that had the 2 phosphates in the center of the helix, and the bases on the outside . In order for DNA's role in genetics to be understood, the bases had to be in the inside of the molecule, which pair up in an exclusive way, and allow DNA to pass genetic information from generation to generation through the copying of the base pattern. Without Rosalind Franklin's insight on the position of the bases, Watson and Crick's would not have discovered the DNA copying mechanism at the time. In addition, embarrassing for Watson during his first model review was the fact that his model was ten times short of required water molecules. Rosalind Franklin presented the DNA water content at the seminar in 1951 that Watson attended. Watson admits that he couldn't concentrate on the lecture, and he began wondering "how she would look if she took of her glasses and did something novel with her hair,,,3 during the seminar. Perhaps if Franklin had been a typical male scientist, Watson would have paid more attention to the lecture, and maybe even taken notes. Furthermore, Rosalind Franklin, and his assistant Gosling published an MRC report, which contained Rosalind's estimations of the 34 Angstrom repeat in the B form of DNA, which was essential for checking the model for the correct pitch of the helix.4 The information in the MRC report was viewed without the consent ofFranklin, adding weight to the immorality of Watson's actions. Even when Franklin contributed this valuable information, 2 Rapoport, Sarah, "Rosalind Franklin: Unsung Hero of the DNA Revolution," The History Teacher 39, no. 1 (November 2002): 120. 3 Watson, James D., The Double Helix (New York: Touchstone, 2001), 69. 4 Manwell, Clyde, "Commentary: The Double Helix: Science and Myth in the Art of Creation," BioScience 29, no. 12 (December 1979): 744. 2 Watson, Crick, and Wilkins did not share the credit for the discovery of DNA's structure with her. Instead, James Watson described in The Double Helix as an obstacle to the discovery since "the point had been reached where Rosy would not even tell Maurice her latest results."s Author Sarah Rapoport affirms, that if Watson, Crick, and Wilkins had properly acknowledged Franklin's contribution, she would have shared the enormous public recognition that they received for discovering the structure ofthe DNA molecule. 6 "Look, there's a helix, and that damned woman just won't see it,"? were the words of Maurice Wilkins, referencing Photograph 51, which he took without her permission. James Watson also repeatedly attacked Rosalind Franklin's position against the helical structure of DNA in The Double Helix, when in fact Franklin did acknowledge the helix as the most probable structure for DNA. James Watson relates, "...since to her mind [Rosalind Franklin's] there was not a shred of evidence that DNA was helical."s James Watson was ignoring Franklin's unpublished notes from 1951 and 1952 that showed that Franklin was certain about the helical nature of wet DNA, but since dry DNA presented a different structure in the X-ray diffraction pictures, she searched for a structure which would explain that patterns produced in both forms. 9 Evidently, Rosalind Franklin's motivation for her research on DNA was not rushing to guess the structure of DNA so that she could win a Nobel Prize, but understanding the scientific reasons behind the discrepancies ofthe two forms ofDNA. Moreover, going back to Franklin's seminar in 1951, Anne Sayre, author ofRosalind Franklin and DNA, assures that "Her notes for the talk, typed, underlined and corrected in her hand, say, 'Conclusion: Big helix in several chains, 5 Watson, James D., The Double Helix (New York: Touchstone, 2001), 56. 6 Rapoport, Sarah, "Rosalind Franklin: Unsung Hero ofthe DA Revolution," The History Teacher 39, no. 1 (November 2002): 117. 7 Manwell, Clyde, "Commentary: The Double Helix: Science and Myth in the Art ofCreation," BioScience 29, no. 12 (December 1979): 743. 8 Watson, James D., The Double Helix (New York: Touchstone, 2001), 165. 9 Shapley, Deborah, "Rosalind Franklin and DNA," New York Times, September 21, 1975, 27. 3 phosphates on the outside, phosphate-phosphate interhelical bonds disrupted by waste links available to proteins,."IO Later on, in 1953, when Rosalind Franklin review the Watson and Crick's final model of DNA, Watson commented that "I feared that her sharp, stubborn mind, caught in her self-made antihelical trap, might dig up irrelevant results that would foster uncertainty about the correctness of the double helix.,,11 Clearly, the stubborn mind belonged to Watson, who once again mistakenly assured that Franklin did not agree with the helix nature of DNA, perhaps thinking that her female intellect was not capable of seeing such plain fact after years ofstudying DNA. Moreover, Rosalind Franklin was more than capable of solving the problem of the structure of DNA, and its role in genetics, without the underhanded actions by Watson and Wilkins. In an interview by Anne Sayre, Francis Crick was asked how long would it have taken for Rosalind Franklin to fully solve the structure of DNA, and he answered, "Perhaps three weeks [after Watson and Crick's publication]. Three months is likelier.,,12 Also, author Brenda Maddox describes Aaron Klug's (Franklin's colleague at Brikbeck College) conclusion that " ...after reviewing her notebooks and reports, which had been given to him [Klug] after her death, both he and Francis Crick became convinced that she was poignantly close.,,13 Franklin and Gosling published their X-ray diffraction research results in the same journal that James Watson and Francis Crick's published their paper on the structure of DNA, but it was too late. Franklin and Gosling's paper was taken just as confirmation for Watson and Crick's discovery. 10 Shapley, Deborah, "Rosalind Franklin and DNA," NewYork Times, September 21, 1975,30. II Watson, James D., The Double Helix (New York: Touchstone, 2001), 210. 12 Manwell, Clyde, "Commentary: The Double Helix: Science and Myth in the Art ofCreation," BioScience 29, no.12 (December 1979): 743. 13 Cohen, Carolyn, "Review: Rosalind Franklin: The Dark Lady ofDNA by Brenda Maddox," The Women's Review ofBooks 20, no. 2 (November 2002): 9. 4 James Watson's portrayal ofRosy in The Double Helix is partially historically inaccurate, with of sexist comments about Franklin personality, and work. Rosalind Franklin was exceptional at both obtaining data, and synthesizing data, due to her unacknowledged discoveries on DNA. The discovery of DNA's double helix structure could not have been possible at the time without Franklin's work, and without her courage to withstand the environment she worked on for all those years at King's College. Moreover, Watson's and Wilkins ideas that Franklin was against the helical structure ofDNA were a product oftheir ignorance towards her approach to science. In contrast to Watson and Crick's trial and error approach to the DNA's structure problem, and their use of science as a medium for recognition, fame and the Nobel Prize, Rosalind Franklin did her work for scientific understanding, and the improvement ofhumanity. 5 Bibliography Cohen, Carolyn. "Review: Rosalind Franklin: The Dark Lady ofDNA by Brenda Maddox." The Women's Review ofBooks 20, no. 2 (November 2002): 9. Manwell, Clyde. "Commentary: The Double Helix: Science and Myth in the Art ofCreation." BioScience 29, no.12 (December 1979): 743-744. Rapoport, Sarah. "Rosalind Franklin: Unsung Hero ofthe DNA Revolution." The History Teacher 39, no. 1 (November 2002): 117, 120. Shapley, Deborah. "Rosalind Franklin and DNA." New York Times, September 21, 1975,27,30. Watson, James D., The Double Helix. New York: Touchstone, 2001, 56, 69, 165-166,210.