PSA Antibody [SPM352] Cat. No.: 34-120 PSA Antibody [SPM352] Specifications HOST SPECIES: Mouse SPECIES REACTIVITY: Human Prostate specific antigen from human sperm plasma was used as the immunogen for this IMMUNOGEN: PSA antibody. TESTED APPLICATIONS: Flow, IF, IHC Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunofluorescence: 0.5-1 ug/ml Immunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) APPLICATIONS: The optimal dilution of the PSA antibody for each application should be determined by the researcher. 1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Properties September 28, 2021 1 https://www.prosci-inc.com/psa-antibody-spm352-34-120.html PURIFICATION: Protein G affinity chromatography CLONALITY: Monoclonal ISOTYPE: IgG1, kappa CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: PBS with 0.1 mg/ml BSA and 0.05% sodium azide CONCENTRATION: 0.2 mg/mL STORAGE CONDITIONS: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles. Additional Info OFFICIAL SYMBOL: KLK3 Prostate-specific antigen, PSA, Gamma-seminoprotein, Seminin, Kallikrein-3, P-30 antigen, ALTERNATE NAMES: Semenogelase, KLK3, APS GENE ID: 354 USER NOTE: Optimal dilutions for each application to be determined by the researcher Background and References Recognizes a single protein of 33-34kDa, identified as the prostate specific antigen (PSA). This mAb is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. BACKGROUND: PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 28, 2021 2 https://www.prosci-inc.com/psa-antibody-spm352-34-120.html.