APPLICATION OF PHOTOSYNTHETIC BACTERIA: BIOCONTROL OF PATHOGENIC ROOT ROT FUNGUS AND OTHER APPLICATIONS （光合成細菌の応用に関する研究） Hongyi Wei 2016 TABLE OF CONTENTS ABSTRACT ............................................................................................................................................................... 1 INTRODUCTION ..................................................................................................................................................... 3 REFERENCES ...................................................................................................................................................... 5 CHAPTER 1 ............................................................................................................................................................... 7 ISOLATION AND CHARACTERIZATION OF PURPLE NON-SULFUR PHOTOSYNTHETIC BACTERIA ................................................................................................................................................................ 7 ABSTRACT ........................................................................................................................................................... 7 INTRODUCTION ................................................................................................................................................. 8 MATERIALS AND METHODS ........................................................................................................................... 9 Sample collection ............................................................................................................................................... 9 Microorganism isolation and cultivation method ........................................................................................... 9 Cell morphology of PSB isolates ..................................................................................................................... 10 Identification of PSB isolates ........................................................................................................................... 11 Accession number ............................................................................................................................................ 12 Pigments Analyses ............................................................................................................................................ 12 Growth ability of PSB isolates ........................................................................................................................ 12 Solid-liquid separation ability ......................................................................................................................... 13 Availability of organic acid by PSB strains ................................................................................................... 13 Statistical analysis ............................................................................................................................................ 14 RESULTS ............................................................................................................................................................. 14 Characterization and identificationof PSB isolates ...................................................................................... 14 Solid-liquid separation ability and growth of PSB Strains .......................................................................... 15 DISCUSSION ....................................................................................................................................................... 15 REFERENCES .................................................................................................................................................... 18 i FIGURES AND TABLES .................................................................................................................................... 21 CHAPTER 2 ............................................................................................................................................................. 34 SWINE WASTEWATER TREATMENT BY APPLYING PHOTOSYNTHETIC BACTERIA STRAIN A ... 34 ABSTRACT ......................................................................................................................................................... 34 INTRODUCTION ............................................................................................................................................... 35 MATERIALS AND METHODS ......................................................................................................................... 36 Sampling and microorganisms ....................................................................................................................... 36 Swine sewage waste water pretreatment ........................................................................................................ 36 Incubation experiment in swine sewage wastewater .................................................................................... 37 Growth rate, VFAs and TOC analyses ........................................................................................................... 37 Ammonia nitrogen, nitrite nitrogen and nitrate nitrogen analyses ............................................................. 37 Statistical analysis ............................................................................................................................................ 38 RESULTS ............................................................................................................................................................. 38 Contents of swine sewage wastewater after pre-treatment .......................................................................... 38 PSB growth in swine sewage wastewater ....................................................................................................... 38 Reduction of VFAs contents ............................................................................................................................ 38 Reduction of TOC ............................................................................................................................................ 39 Reduction of ammonia nitrogen ..................................................................................................................... 39 DISSCUSSION ..................................................................................................................................................... 39 REFERENCES .................................................................................................................................................... 43 FIGURES AND TABLES .................................................................................................................................... 47 CHAPTER 3 ............................................................................................................................................................. 52 ENHANCEMENT OF ISOLATED BACTERIA IN THEIR ANTIFUNGAL ACTIVITIES ON ROOT ROT FUNGI BY APPLYING PHOTOSYNTHETIC BACTERIA .............................................................................. 52 ABSTRACT ......................................................................................................................................................... 52 INTRODUCTION ............................................................................................................................................... 53 ii MATERIALS AND METHODS ............................................................................................................................. 54 Sample collection ............................................................................................................................................. 54 Isolation, cultivation and identification of microorganisms ......................................................................... 54 Accession number ............................................................................................................................................ 56 Antagonistic test of microorganisms to pathogenic root rot fungus ............................................................ 56 Preliminary test ................................................................................................................................................ 56 Antagonistic test using mycelial disk placement method ............................................................................. 57 RESULTS ................................................................................................................................................................. 57 Isolation and identification of the microorganisms....................................................................................... 57 Preliminary test of antagonistic activity to pathogenic root rot fungus ...................................................... 58 Antagonistic test of the bacterial isolates by using the mycelial disk placement method .......................... 58 DISCUSSION ........................................................................................................................................................... 59 REFERENCES .......................................................................................................................................................