Anti-KHDRBS1 (aa 331-348) polyclonal antibody (CABT-B1716) This product is for research use only and is not intended for diagnostic use. PRODUCT INFORMATION Specificity This antiserum (also known as AD-1 or AD1) recognizes Sam68, but not two other GSG (GRP33, Sam68, GLD-1) proteins, the Sam68-like mammalian proteins SLM-1 and SLM-2. Immunogen KLH-conjugated peptide corresponding to amino acid residues 331-348 of human Sam 68 Isotype IgG Source/Host Rabbit Species Reactivity Human, Mouse Purification Unpurified Conjugate Unconjugated Applications WB, IP, ICC, IHC Epitope aa 331-348 Molecular Weight ~68 kDa observed Format Liquid Concentration Please refer to lot specific datasheet. Size 200 µL Buffer Serum with 0.05% sodium azide. Preservative 0.05% Sodium Azide Storage Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. BACKGROUND 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 1 © Creative Diagnostics All Rights Reserved Introduction KH domain-containing, RNA-binding, signal transduction-associated protein 1 (UniProt Q60749; also known as GAP-associated tyrosine phosphoprotein p62, Khdrbs1,p68, p21 Ras GTPase- activating protein-associated p62, Sam68, Src-associated in mitosis 68 kDa protein) is encoded by the Khdrbs1 gene (Gene ID 20218) in murine species. Sam68 is a member of the "signal transduction and activation of RNA" (STAR) family of KH domain-containing RNA-binding proteins that mediates pre-mRNA splicing, mRNA export, and protein translation. It binds to UAAA, UUUA, and UUUUUU motifs with high affinity. RNA immunoprecipitation (RIP) techniques using lysates from UV cross-linked NIH 3T3 cells have identified 23 Sam68 complex-associated mRNA species, including Thrap1, bri3bp, Myl6, Copeb/KLF6/Zf9, Hmgn1, Smc6, and biglycan. In addition, Sam68 is found to be a key regulator of Nrxn1 alternative splicing in the central nervous system in a neuronal activity-dependent manner. Sam68 is phosphorylated on three tyrosine residues (pTyr435, pTyr440, and pTyr443) within the C-terminal nuclear localization signal (NLS) region by the breast tumor kinase (BRK) following EGF stimulation of breast tumor cells with pTyr440 being a principal modulator of Sam68 nuclear localization. Studies comparing wild-type and Sam68-/- knockout mice revealed a positive regulatory role of Sam68 toward adipocyte differentiation, as well as a negative impact of Sam68 against osteoblast differentiation among aged mice. GENE INFORMATION Entrez Gene ID 20218 UniProt ID Q60749 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 2 © Creative Diagnostics All Rights Reserved.