Stock boundaries for spotted seatrout (Cynoscion nebulosus) in Florida based on population genetic structure Item Type monograph Authors Seyoum, Seifu; Tringali, Michael D.; Barthel, Brandon L.; Villanova, Vicki; Puchulutegui, Cecilia; Davis, Michelle C.; Alvarez, Alicia C. Publisher Florida Fish and Wildlife Conservation Commission, Fish and Wildlife Research Institute Download date 04/10/2021 17:27:27 Link to Item http://hdl.handle.net/1834/41131 Florida Fish and Wildlife Research Institute TECHNICAL REPORTS Stock Boundaries for Spotted Seatrout (Cynoscion nebulosus) in Florida Based on Population Genetic Structure Seifu Seyoum, Michael D. Tringali, Brandon L. Barthel, Vicki Villanova Cecilia Puchulutegui, Michelle C. Davis, Alicia C. Alvarez Rick Scott Governor of Florida Florida Fish and Wildlife Conservation Commission Nick Wiley Executive Director The Fish and Wildlife Research Institute (FWRI) is a division of the Florida Fish and Wildlife Conservation Commission (FWC). The FWC is “managing fish and wildlife resources for their long-term well-being and the benefit of people.” The FWRI conducts applied research pertinent to managing fishery resources and species of special concern in Florida. Programs at FWRI focus on obtaining the data and information that managers of fish, wildlife, and ecosystem resources need to sustain Florida’s natural resources. Topics include managing recreationally and commercially important fish and wildlife species; preserving, managing, and restoring terrestrial, freshwater, and marine habitats; collecting information related to population status, habitat requirements, life history, and recovery needs of upland and aquatic species; synthesizing ecological, habitat, and socioeconomic information; and developing educational and outreach programs for classroom educators, civic organizations, and the public. The FWRI publishes three series: Memoirs of the Hourglass Cruises, Florida Marine Research Publications, and FWRI Technical Reports. FWRI Technical Reports contain information relevant to immediate resource-management needs. Gil McRae, FWRI Director Bland Crowder, Shad Run, Word and Graphic Editor Stock Boundaries for Spotted Seatrout (Cynoscion nebulosus) in Florida Based on Population Genetic Structure Seifu Seyoum Michael D. Tringali Brandon L. Barthel Vicki Villanova Cecilia Puchulutegui Michelle C. Davis Alicia C. Alvarez Florida Fish and Wildlife Conservation Commission Florida Fish and Wildlife Research Institute 100 Eighth Avenue Southeast St. Petersburg, Florida 33701 Florida Fish and Wildlife Conservation Commission FWRI Technical Report 18 2014 Cover Photograph Spotted Seatrout in net — By Tim Donovan, FWC Copies of this document may be obtained from MyFWC.com Florida Fish and Wildlife Conservation Commission Fish and Wildlife Research Institute 100 Eighth Avenue SE St. Petersburg, FL 33701-5095 Attn: Librarian Document Citation Seyoum, S., M.D. Tringali, B.L. Barthel, V. Villanova, C. Puchulutegui, M.C. Davis, and A. C. Alvarez. 2014. Stock boundaries for spotted seatrout (Cynoscion nebulosus) in Florida based on population genetic structure. Fish and Wildlife Research Institute Technical Report TR-18 + 26 p. Document Production This document was composed in Microsoft Word® and produced using InDesign® on Apple Macintosh® computers.The headline font is Adobe® Avant Garde, the text font is Adobe® Palatino, and the cover headline is Adobe® Avant Garde. The cover and text papers used in this publication meet the minimum requirements of the American National Standard for Permanence of Paper for Printed Library Materials Z39.48—1992. Florida Fish and Wildlife Research Institute Technical Report TR-18. Table of Contents ACKNOWLEDGMENTS.................................................................................................................................... 6 EXECUTIVE SUMMARY.................................................................................................................................... 7 INTRODUCTION................................................................................................................................................ 8 METHODS.......................................................................................................................................................... 10 Sampling and DNA Extraction................................................................................................................ 10 Development of Microsatellite Markers.................................................................................................... 10 Microsatellite Genotyping........................................................................................................................ 10 Data Analyses........................................................................................................................................... 11 Phenetic Clustering.................................................................................................................................. 11 Genetic Structure..................................................................................................................................... 11 RESULTS............................................................................................................................................................. 12 Microsatellite Marker Assays................................................................................................................... 12 Standard Genetic Measures and Distances ............................................................................................. 12 Phenetic Clustering.................................................................................................................................. 12 Analysis of Molecular Variance (AMOVA)............................................................................................. 17 Bayesian Population Assignment............................................................................................................. 17 Mantel Test............................................................................................................................................... 19 IMPLICATIONS FOR THE FISHERY............................................................................................................. 19 FWC Spotted Seatrout Regional Boundaries........................................................................................... 19 Genetic Regional Boundaries.................................................................................................................... 19 Incongruencies Between the Boundaries of FWC Management Units and the Genetic Stocks................21 Stock assessment of the Spotted Seatrout in Florida................................................................................ 21 Recommendations..................................................................................................................................... 21 LITERATURE CITED......................................................................................................................................... 23 FIGURES................................................................................................................................................................... Figure 1. Locations and abbreviations of 18 Spotted Seatrout sampled for this study........................................ 10 Figure 2. Unrooted neighbor-joining phenogram................................................................................................ 14 Figure 3. Mean likelihood L determined from 10 replicates of each value of K.................................................... 17 Figure 4. Genetic population structure among Spotted Seatrout samples.......................................................... 18 Figure 5. Fish and Wildlife Conservation Commission Spotted Seatrout stock boundaries............................... 20 Figure 6. Mantel tests between pairwise genetic and geographic distances........................................................ 20 TABLES..................................................................................................................................................................... Table 1. Characterization of 29 polymorphic microsatellite loci.......................................................................... 13 Table 2. Average standard measures of genetic diversity..................................................................................... 15 Table 3. Estimates of genetic and geographic distances in Spotted Seatrout samples......................................... 15 Table 4. Analysis of molecular variance for 18 Spotted Seatrout samples........................................................... 16 Acknowledgments We thank the staff of the Fisheries Independent Monitoring Program at the Tequesta Field Laboratory of the Florida Fish and Wildlife Research Institute for collecting the samples. We are especially grateful to Caitlin Curtis for help in the development of the microsatellite markers, Joel Bickford and Sarah Walters for collecting samples, and Mike Murphy and Bob Muller for insightful comments on a draft of this report. This work was supported by the U.S. Department of the Interior, U.S. Fish and Wildlife Service, under the Federal Aid in Sport Fish Restoration Program, Grant F-69. The statements, findings, and conclusions are those of the