Transcription Profile of HTLV-I-Infected Cells Compared to Activated Lymphocytes Cynthia A. Pise-Masison1, Michael Radonovich1, Renaud Mahieux2, Pramita Chatterjee1, Craig Whiteford3, Janet Duvall1, Claire Guillerm1, Antoine Gessain2, and John N. Brady1* 1Basic Research Laboratory, Virus Tumor Biology Section, National Cancer Institute, 9000 Rockville Pike, Bethesda, MD 20892, 2Unite d'Epidemiologie et Physiopathologie des Virus Oncogenes, Batiment SIDA-Retrovirus, 28, rue du Dr Roux, Institut Pasteur, 75724 Paris cedex 15, 3ATC, National Institutes of Health, Gaithersburg, MD, 20805 *Corresponding Author: [email protected]; tel.:(301)496-0986; fax.:(301)496-4951 [email protected]; tel: (301)435-2499; fax: (301)496-4951 [email protected]; tel: (301)496-4953;fax: (301)496-4951 [email protected]; tel: (33)-1-45-68-89-06;fax: (33)-1- 40-61-34-65 [email protected]; tel: (301)496-4793;fax: (301)496-4951 [email protected]; tel: (301)402-9217;fax: (301)496-4951 [email protected]; tel: (301)435-2501; fax: (301)496-4951 [email protected]; tel: (301)496-0988;fax: (301)496-4951 [email protected]; tel: (33)-1-45-68-89-06;fax: (33)-1- 40-61-34-65 Running title: Gene Expression Profiles of HTLV-I-Infected Cells Key Words: HTLV-I, ATL, Transcription, Microarray, Gene Expression 1 Supplementary Table. A list of the 763 differentially expressed genes in the HTLV-I-infected cells compared to activated donor peripheral blood lymphocytes (PBLs), listed by category. All genes listed were expressed either 2-fold or greater (increased) or 2-fold or less (decreased) in comparison to activated PBLs. Indicated by gray shading, genes already identified as differentially expressed in microarray studies are listed. GenBank Acc. No. Gene Description Citationa Harhaj de La Ruckes et.al. Fuente et.al. et. al. Increased Genes Transcription factors AD000092_cds7_s_at AF005887_at (ATF6) CREB binding serum response factor D14826_s_at (CREM) cAMP responsive element modulator D87673_at (HSF4) heat shock transcription factor 4 D90070_s_at (APR) PMA induced mRNA D90224_at (TNFSF4) tumor necrosis factor (ligand) superfamily up up gp34 J04076_at early growth response 2 (Krox-20 homolog) up J04102_at ETS2 up J04111_at c-Jun up L19871_at (ATF3) activating transcription factor 3 L23587_at DP-1 up L34587_at putative gene up L37936_at elongation factor Ts L40386_s_at DP-2 L46353_at HMG1-C M16038_at Lyn M37583_at (H2AFZ) M57732_at transcription factor 1, hepatic; LF-B1, HNF1 M61156_at (TFAP2A) transcription factor AP-2 alpha M62810_at transcription factor 6-like 1 up M69043_at (NFKBIA) IKB alpha M74719_at (TCF4) transcription factor 4 M77810_at GATA-2 up M92357_at B94 up S73205_at (INSAF) insulin activator factor U15460_at B-ATF U20734_s_at JunB up U24166_at EB1 U51127_at (IRF5) interferon regulatory factor 5 U52682_at (IRF4) interferon regulatory factor 4 U59863_at (I-TRAF) U91616_at (NFKBIE) cytoplasmic NFkB inhibitor epsilon Receptors/Signaling molecules 2 D26070_at (ITPR1) inositol 1,4,5-triphosphate receptor, type 1 D38076_at (RANBP1) D38128_at (PTGIR) prostaglandin I2 (prostacyclin) receptor (IP) D49489_at The transcript is amplified in hydroxyurea-resistant cells D50678_at (LRP8) low density lipoprotein receptor-related protein 8 J03258_at (VDR) vitamin D (1,25- dihydroxyvitamin D3) receptor K02405_f_at prepeptide MHC HLA-DC3-beta L08069_at (HSJ2) heat shock protein DNAJ-like 2 L08096_at (TNFSF7) CD27 ligand up L08187_at (EBI3) up L08488_at (INPP1) inositol polyphosphate-1-phosphatase L12723_at (HSPA4) heat shock 70kD protein 4 L13740_at TR3 orphan receptor L19872_at (AHR) aryl hydrocarbon receptor L38608_at (ALCAM) CD6 ligand L40357_at (TRIP7) thyroid hormone receptor interactor 7 L40379_at (TRIP10) thyroid receptor interacting protein 10 L77886_at (PTPRK) protein tyrosine phosphatase, receptor type, K M11058_at (HMGCR)3-hydroxy-3-methylglutaryl-Coenzyme A reductase M11749_at Thy-1 glycoprotein M16276_at (HLA-DQB1) MHC, class II, DQ beta 1 M16441_cds1_ (TNF) tumor necrosis factor (TNF superfamily, member 2) at M22382_at (HSPD1) heat shock protein 60kDa up M23197_at (CD33) M24283_at (ICAM1) intercellular adhesion molecule 1 (CD54) up M26958_at (PTHrP) M33600_f_at (HLA-DRB5) M34996_at cell surface glycoprotein class II M35128_at (CHRM1) cholinergic receptor, muscarinic 1 M57466_s_at (HLA-DPB1) MHC, class II, DP beta 1 M59465_at (A20) TNFAIP1 M62424_at (F2R) coagulation factor II receptor up M83554_at (TNFRSF8) TNF receptor superfamily, member 8 up CD30 M83664_at (HLA-DPB1) MHC, class II, DP beta 1 M83738_at (PTPN9) protein tyrosine phosphatase, non-receptor type 9 M84349_at (CD59) U03398_at (TNFSF9) ILA ligand (TNF related) U07550_at (HSPE1) heat shock protein 10kDa U10550_at (gem) GTPase U12767_at (MINOR) U29343_at (HMMR) hyaluronan-mediated motility receptor (RHAMM) U31628_at (IL15RA) interleukin 15 receptor, alpha U33017_at (SLAM) signaling lymphocytic activation molecule U37546_s_at (API1) apoptosis inhibitor 1 U45983_at (CCR8) U64198_at (IL12RB2) interleukin 12 receptor, beta 2 U68030_at (CCR6) up U70426_at (RGS16) regulator of G-protein signalling 16 U72761_at (KPNB3) beta 3 karyopherin (importin) 3 X00274_at (HLA-DRA) up X01057_at (IL2RA) interleukin 2 receptor, alpha up up X01060_at (TFRC) transferrin receptor (p90, CD71) up X02317_at (SOD1) superoxide dismutase up up X02419_rna1_a (PLAU) plasminogen activator, urokinase t X02530_at (INP1-) IFN-induced protein down X02910_at TNF alpha down X03068_f_at precursor class II antigen X03100_cds2_ (HLA-SB) MHC, class II antigen beta at X62744_at (HLA-DM) MHC, class II, DM alpha X77584_at (TXN) thioredoxin Y00636_at (CD58) Z97074_at Rab9 effector GTPase p40 up Kinases/Phosphatases AB003698_at (CDC7L1) D10495_at (1-May) Protein Kinase C delta up up D10522_at (MACS) MARCKS, 80K-L up J02902_at protein phosphatase 2A regulatory alpha subunit L08488_at (INPP1) inositol polyphosphate-1-phosphatase L08835_rna2_a (DMPK) dystrophia myotonica-protein kinase t L11329_at (DUSP2) dual specificity phosphatase 2 up L20320_at (CDK7) cyclin-dependent kinase 7 L37043_at (CSNK1E) casein kinase 1, epsilon L76927_rna1_a (GALK1) galactokinase 1 t L77886_at (PTPRK) protein tyrosine phosphatase, receptor type, K M68520_at (CDK2) cyclin-dependent kinase 2 M80359_at (MARK3) MAP/microtubule affinity-regulating kinase 3 U15932_at (DUSP5) dual specificity phosphatase 5 up U37022_rna1_a (CDK4) cyclin-dependent kinase 4 t U43522_at (PTK2B) protein tyrosine kinase 2 beta U48296_at (PTP4A1) protein tyrosine phosphatase type IVA, member 1 U48807_at (DUSP4) dual specificity phosphatase 4 U59919_at (SMAP) Smg GDS-associated protein X02419_rna1_a (PLAU) plasminogen activator, urokinase t X17620_at (NME1) non-metastatic cells 1, protein (NM23A) X54941_at (CKS1) CDC28 protein kinase 1 X54942_at (CKS2) CDC28 protein kinase 2 X56468_at 14.3.3 protein tau X56494_at (PKM2) pyruvate kinase, muscle X89416_at (PPP5) Y00815_at (PTPRF) protein tyrosine phosphatase, receptor type, F Y07604_at (NME4) non-metastatic cells 4 Y10275_at (PSP) phosphoserine phosphatase Z18951_at (CAV1) caveolin 1, caveolae protein, 22kD Cytokines/interleukins/oncogenes 4 D43767_at (SCYA17) small inducible cytokine subfamily A (Cys- up Cys) J00219_at (IFNG) interferon gamma up J04156_at (IL7) interleukin 7 L00058_at c-myc protein, second translated L08187_at (EBI3) cytokine receptor up M13207_at (CSF2) GM-CSF M13755_at (ISG15) IFN-induced 15kDa protein up M14745_at bcl-2 protein up M26062_at (IL2RB) interleukin 2 receptor, beta M28130_at (IL8) interleukin 8 M28209_at (RAB1) RAB1, member RAS oncogene family M28215_at (RAB5A) GTP-binding protein up M30135_at (P40) T-cell and mast cell growth factor precursor M37435_at (CSF1) M57506_rna1_ (SCYA1) I-309 up up up at M57731_at (GRO3) GRO3 oncogene M59941_at (CSF2RB) GM-CSF receptor beta chain M65291_at (IL12A) interleukin 12 A U01038_at (pLK) polo-like kinase up U03397_at (TNFRSF9) ILA U11877_at (IL8RB) interleukin 8 receptor beta U14407_at (IL15) interleukin 15 U31120_rna1_a (IL13) interleukin 13 up t U31628_at (IL15RA) interleukin 15 receptor, alpha U64198_at (IL12RB2) interleukin 12 receptor, beta 2 U83171_at (SCYA22) small inducible cytokine subfamily A (Cys-Cys) X01057_at (IL2RA) interleukin 2 receptor, alpha up up X04602_s_at (IL6) interleukin 6 (interferon, beta 2) up X12794_at (ERBAL2) v-erb-a X51345_at (JUNB) jun B proto-oncogene up 681_s_at (JUND) jun D proto-oncogene X58298 (IL6R) interleukin 6 receptor X67951_at (PAGA) proliferation-associated gene A X75042_at (REL) v-rel avian reticuloendotheliosis viral oncogene up up homolog Y00081_s_at BSF-2/IL6 gene Y10659_at (IL13RA1) interleukin 13 receptor alpha 1 Cell cycle/apoptosis/repair AF005775_at (CFLAR) CASP8 and FADD-like apoptosis regulator D00017_at (ANX2) annexin II D13639 cyclin D2 up up D14878_at (D123) D123 gene product S-phase entry regulator up D21063_at (MCM2) minichromosome maintenance deficient 2 (mitotin) D55716 (P1cdc47) MCM7 D84557_at (HsMcm6) J04611_at (G22P1) 70kDa KU autoantigen J05614_at PCNA L07493_at (RPA3) replication factor 5 L07541_at (RFC3) replication factor 3 up L20320_at (CDK7) cyclin-dependent kinase 7 L25876_at (CIP2) associated with cyclin-dependent kinase M15796_at PCNA M16441_cds1_ (TNF) tumor necrosis factor (TNF superfamily, down at member 2) M17754_at (BN51T) BN51 (BHK21) M25753_at cyclin B1 up up M34065_at (CDC25C) cell division cycle 25C M36089_at (MSH2) X-ray repair complementing defective repair protein up M60974 GADD45 up up M68520_at (CDK2) cyclin-dependent 2 M74093_at (CCNE1) cyclin E1 M87339_at