TOXICOLOGY ANALYTICAL METHODS Services ForensicCopy Police Internet DOCUMENT State Idaho of Uncontrolled OBSOLETE Property Toxicology Analytical Methods Revision 5 Table of Contents Issue Date: 08/07/2017 Page 1 of 210 Issuing Authority: Quality Manager All printed copies are uncontrolled Table of Contents Revision History ............................................................................................................................................ 7 Revision # ...................................................................................................................................................... 7 Description of Changes ................................................................................................................................. 7 Toxicology AM #1: Enzyme Immunoassay Screening for Drugs-of-Abuse in Urine ...................................... 8 1.0 Background/References ................................................................................................................ 8 2.0 Scope ........................................................................................................................................... 10 3.0 Equipment/Reagents .................................................................................................................. 11 4.0 Procedure .................................................................................................................................... 12 Toxicology AM #2: General Extraction of Urine for Basic/Neutral or Acidic/NeutralServices Compounds ............ 15 1.0 Background/References .............................................................................................................. 15 2.0 Scope ........................................................................................................................................... 15 3.0 Equipment/Reagents .................................................................................................................. 15 4.0 Procedure ....................................................................................................................................ForensicCopy 16 5.0 Work Instructions ........................................................................................................................ 19 Toxicology AM #3: Qualitative 11-nor-9-THC-D9-COOHPolice (Carboxy-THC) in Urine ...................................... 21 1.0 Background/References ..............................................................................................................Internet 21 2.0 Scope ...........................................................................................................................................DOCUMENT 22 3.0 Equipment/Reagents ..................................................................................................................State 22 4.0 Procedure .................................................................................................................................... 24 5.0 Work Instructions ........................................................................................................................Idaho 27 Toxicology AM #4: Cocaine and Cocaine Metabolites in Urine .................................................................. 28 of Uncontrolled 1.0 Background/References ..............................................................................................................OBSOLETE 28 2.0 Scope ........................................................................................................................................... 29 3.0 Equipment/Reagents .................................................................................................................. 29 4.0 PropertyProcedure .................................................................................................................................... 31 5.0 Work Instructions ........................................................................................................................ 34 Toxicology AM #5: Qualitative Benzodiazepines and Ancillary Compounds in Urine ................................ 37 1.0 Background/References .............................................................................................................. 37 2.0 Scope ........................................................................................................................................... 39 3.0 Equipment/Reagents .................................................................................................................. 39 4.0 Procedure .................................................................................................................................... 41 Toxicology Analytical Methods Revision 5 Table of Contents Issue Date: 08/07/2017 Page 2 of 210 Issuing Authority: Quality Manager All printed copies are uncontrolled 5.0 Work Instructions ........................................................................................................................ 44 6.0 Comments ................................................................................................................................... 47 Toxicology AM #6: Screening for Gamma-Hydroxybutyrate (GHB) in Urine .............................................. 48 1.0 Background/References .............................................................................................................. 48 2.0 Scope ........................................................................................................................................... 50 3.0 Equipment/Reagents .................................................................................................................. 50 4.0 Procedure .................................................................................................................................... 51 5.0 Work Instructions ........................................................................................................................ 54 Toxicology AM #7: Enzyme-Linked Immunosorbent Assay (ELISA) Screening for Drugs of Abuse ............ 56 1.0 Background/References ..............................................................................................................Services 56 2.0 Scope ........................................................................................................................................... 57 3.0 Equipment/Reagents .................................................................................................................. 58 4.0 Procedure .................................................................................................................................... 61 5.0 Comments ...................................................................................................................................ForensicCopy 66 Toxicology AM #8: Basic and Neutrals Drugs in Blood ............................................................................... 68 1.0 Background/References .............................................................................................................. 68 2.0 Scope ...........................................................................................................................................Police 68 Internet 3.0 Equipment/Reagents .................................................................................................................. 69 DOCUMENT 4.0 Procedure ....................................................................................................................................State 70 5.0 Work Instructions ........................................................................................................................ 74 Toxicology AM #9: Acidic andIdaho Neutral Drugs in Blood ................................................................................ 76 1.0 Background/Referencesof .............................................................................................................. 76 2.0 Scope ...........................................................................................................................................Uncontrolled 76 OBSOLETE 3.0 Equipment/Reagents .................................................................................................................. 76 4.0 Procedure .................................................................................................................................... 78 5.0 PropertyWork Instructions ........................................................................................................................ 80 Toxicology AM #10: Methamphetamine and Amphetamine in Blood ....................................................... 82 1.0 Background/References .............................................................................................................. 82 2.0 Scope ........................................................................................................................................... 83 3.0 Equipment/Reagents .................................................................................................................. 83 4.0 Procedure .................................................................................................................................... 86 5.0 Work Instructions .......................................................................................................................