SGCE Rabbit Pab

SGCE Rabbit Pab

Leader in Biomolecular Solutions for Life Science SGCE Rabbit pAb Catalog No.: A5330 Basic Information Background Catalog No. This gene encodes the epsilon member of the sarcoglycan family. Sarcoglycans are A5330 transmembrane proteins that are components of the dystrophin-glycoprotein complex, which link the actin cytoskeleton to the extracellular matrix. Unlike other family Observed MW members which are predominantly expressed in striated muscle, the epsilon 55kDa sarcoglycan is more broadly expressed. Mutations in this gene are associated with myoclonus-dystonia syndrome. This gene is imprinted, with preferential expression from Calculated MW the paternal allele. Alternatively spliced transcript variants encoding different isoforms 49kDa/51kDa/52kDa have been found for this gene. A pseudogene associated with this gene is located on chromosome 2. Category Primary antibody Applications WB,IHC,IF Cross-Reactivity Human, Mouse Recommended Dilutions Immunogen Information WB 1:500 - 1:2000 Gene ID Swiss Prot 8910 O43556 IHC 1:50 - 1:200 Immunogen 1:50 - 1:200 IF Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human SGCE (NP_003910.1). Synonyms SGCE;DYT11;ESG;epsilon-SG Contact Product Information www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts of various cell lines, using SGCE antibody (A5330) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. Immunohistochemistry of paraffin- Immunofluorescence analysis of HeLa cells embedded mouse heart using SGCE using SGCE antibody (A5330). Blue: DAPI antibody (A5330) at dilution of 1:200 (40x for nuclear staining. lens). Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..

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