Contamination in Daphnia Culture Samples: A5, B5, C6, F2, F6, G4 Fig. 1 Phylogenetic analysis (PhyML v.3.0.1) of the 6 samples. MSA: 10 20 30 40 50 60 70 80 90 100 ----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----| Con AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGTATGCCTTACACATGCAAGTCGAACGGTAGAGGgGCAACCCTtGAG--n-AGTGGCGAACGGGTG A5 .................................................................................----............... F6 .................................................................................----...............Primer fD1 C6 .................................................................................----............... G4 ....................................................................A....T...C...----............... F2 .....................AC............C.G..T.A..................------------...CGC.AGGGG......AG....... B5 ....................GG.............CG...T.A.G................--------...GT.T.C.GACTGT.......C....... 110 120 130 140 150 160 170 180 190 200 ----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----| Con AGTAATACATCGnGAACGTGCCCAGTCGTGGGGGATAACGTAGCGAAAGCTACGCTAATACCGCATACGAnnnnnnnnCCTGAGGGTGAAAGCGGGGGAt A5 ............-.........................................................--------...................... F6 ............-.........................................................--------...................... C6 ............-.........................................................--------...................... G4 ............-.........................................................--------.....................C F2 .....CG.G.G.-..........TTCGC.TC..A...G.CCC.G....CTGGGAG........G.....C--------.G.ATT..-------------- B5 .....CG.G.AACTG..C.A...CAAA..C.C........GTT......AAT.........GTG..GT.CTGCTCCCT....T...ATC.G--------- 210 220 230 240 250 260 270 280 290 300 ----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----| Con CGcAAGACCTCGCGCGATTGGAGCGGCCGATGgCAGATTAGgTAGTTGGTGGGGTAAAGGCCTACCAAGcCTgCGATCTGTAGCTGGTCTGAGAGGACGA A5 .................................................................................................... F6 .................................................................................................... C6 .................................................................................................... G4 ..T...G........................AT........C................A...C......G.GA........................... F2 -.A....TT.AT..GCGAA...T.....CGC.TTG......C.......A..T....T..A.CC.....G..A.....CA.................T.. B5 -TA...GTT.ACT..TT.G...TG..GTTGC.TTCC..C..C........A..................G.GA...CGGA....C..C........GT.G 310 320 330 340 350 360 370 380 390 400 ----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----| Con CCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCAGCCATACCGCGTG A5 .................................................................................................... F6 .................................................................................................... C6 .................................................................................................... G4 .......................................................................A......T.............G....... F2 T................................................................................................... B5 ..G......GG..C............G..CC.....................TA.....C..CC..........A.........GG...G.CG....... 410 420 430 440 450 460 470 480 490 500 ----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----| Con CGGGAAGAAGGCCTTCGGGTTGTAAACCGCTTTTGTCAGGGAAGAAAT--nCTTCTGGGCTAATACCTCGGGAGGATGACGGTACCTGAAGAATAAGCAC A5 ................................................---................................................. F6 ................................................---................................................. C6 ................................................---................................................. G4 ...............................................CGAGT....---.......AGA.ACTTA......................... F2 ................................................---................................................. B5 A....T.....TT.....A.C.......T.-.GAA.......C....GACG...-------------.AT.CG................GT...-..... 510 520 530 540 550 560 570 580 590 600 ----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----| Con CGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTCCATAAGACAGAT A5 .................................................................................................... F6 .................................................................................................... C6 .................................................................................................... G4 .......................................................................................ATGC........G F2 .................................................................................................... B5 .........C........................G...............CC......C.............G.C.T.T......GATGT....T.T.G. 610 620 630 640 650 660 670 680 690 700 ----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----| Con GTGAAATCCCCGGGCTCAACCTGGGAACTGCATTTGTGACTGTGGGGCTAGAGTACGGTAGAGGGGGATGGAATTCCGCGTGTAGCAGTGAAATGCGTAG A5 .................................................................................................... F6 .................................................................................................... C6 .................................................................................................... G4 ...............................C..........CATA............C......................................... F2 ........................................................Y........................................... B5 T.T...GA.TGC.........GCA.GGA..G.C.G.AT....GCATT..T..CCT.T.G....A.AAC.........TG.......G...G......... 710 720 730 740 750 760 770 780 790 800 ----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----| Con ATATGCGGAGGAACACCAATGGCGAAGGCAATCCCCTGGACCTGTACTGACGCTCATGCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAG A5 .................................................................................................... F6 .................................................................................................... C6 .................................................................................................... G4 .................G.....................G............................................................ F2 .................................................................................................... B5 ...CCA........................GGTT.T.....AGAAGG.......G.G..G......T.........G...C.............G..... 810 820 830 840 850 860 870 880 890 900 ----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----| Con TCCACGCCCTAAACGATGTCAACTGGTTGTTGGGAGGGTTTCTTCTCAGTAACGAAGCTAACGCGTGAAGTTGACCGCCTGGGGAGTACGGCCGCAAGGT A5 .................................................................................................... F6 .................................................................................................... C6 .................................................................................................... G4 ......................................................T............................................. F2 ...........................................................................................T......A. B5 .....A.............ACGT...C.AA.C.C...A.G--C.G.G.T.GG.............AT..ACGT..........A................ 910 920 930 940 950 960 970 980 990 1000 ----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----| Con TGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGATGATGTGGTTTAATTCGATGCAACGCGAAAAACCTTACCTACCCTTGACATGGCAGGA A5 .................................................................................................... F6 .................................................................................................... C6 .................................................................................................... G4 ..............................................................................................C..... F2 .A................................................A................................................. B5 ........................G.................GC................A..........G.........AGGT.........CT.... 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 ----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----|----:----| Con ATCCCGCAGAGATGTGGGAGTGCTCGAAAGAGAACCTGCACACAGGTGCTGCATGGCCGTCG A5 .............................................................. F6 .............................................................. C6 .............................................................. G4 ....T.................................G....................... F2 .............................................................. B5 .C.TTT........A.A.G....C.TTCG.G......AG..................T.... Sequence Analysis RDP Search for A5: Results for Query Sequence: A5_, 997 unique oligos no rank Root (20) (match sequences) domain Bacteria (20) phylum