Population dynamics of the invasive green mussel Perna viridis and their response to the toxic dinoflagellate Karenia brevis: application of Dynamic Energy Budget theory to determine population trends Katherine Mcfarland To cite this version: Katherine Mcfarland. Population dynamics of the invasive green mussel Perna viridis and their re- sponse to the toxic dinoflagellate Karenia brevis: application of Dynamic Energy Budget theory to determine population trends. Ecology, environment. Université de Bretagne Occidentale (UBO), Brest, 2015. English. tel-01252504 HAL Id: tel-01252504 https://hal.univ-brest.fr/tel-01252504 Submitted on 11 Jan 2016 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. 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Distributed under a Creative Commons Attribution| 4.0 International License THÈSE / UNIVERSITÉ DE BRETAGNE OCCIDENTALE sous le sceau de l’Université européenne de Bretagne présentée par pour obtenir le titre de Katherine McFarland DOCTEUR DE L’UNIVERSITÉ DE BRETAGNE OCCIDENTALE Préparée au Laboratoire des Sciences de la Mer Mention : Biologie Marine Institute Universitaire Européen de la Mer et École Doctorale des Sciences de la Mer Florida Gulf Coast University Mention : (à préciser) Population dynamics of the invasive green mussel Thèse soutenue le (18/05/2015) École Doctorale (à préciser) devant le jury composé de : Perna viridis and their response to the toxic dinoflagellate Karenia brevis: application of Dynamic Fred JEAN Maître de Conférences, LEMAR / Directeur de these Energy Budget theory to determine population trends Aswani K. VOLETY Dean of Arts and Sciences, University of North Carolina Wilmington / Directeur Scientifique Philippe SOUDANT Directeur de Recherche, CNRS/ Examinateur Sebastien LEFEBVRE Professeur / Université de Lille 1 / Rapporteur Xavier de MONTAUDOUIN Maître de Conférences / Université de Bordeaux / Rapporteur Pedro Reis COSTA Ph D Reasercher / Instituto Português do Mar e da Atmosfera, Lisbon, Portuga/ Examinateur To my Grandfathers Dr. Robert McFarland and Dr. John McCoy How I wish you were here Acknowledgements I first and foremost would like to thank my two co-advisors, Dr. Aswani K. Volety and Dr. Fred Jean for their support and patience throughout this journey. You he both been incredible mentors and I am forever grateful for the countless hours spent helping through the many bumps along the road. Aswani thank you for pushing me to continuously improve, the encouragement step out of my comfort zone (quite frequently) and the support to succeed. Thank you for your patience on the days when I would come roaring into your office like a mad man. With calm, and sometime quick discussions you were always able to get my head back on straight and my nerves settled down. This project and dissertation would not have been possible without your endless support. Fred, what a journey DEB has been. I don’t know who told you I was a modeling person, but man was I in for a surprise! Thank you for all your patience with my very steep learning curve and often frantic emails. Even when I was still incredibly confused, with a simple French “phhh” you were able to renew my motivation to defeat my problems with DEB or at least keep trying... To my committee, Drs. Philippe Soudant, Julien Thébault and Ronald Toll for aid in planning of projects, editing of manuscripts and support throughout this program. Special thanks to Philippe for the endless hours of discussing data and detailed editing and re-editing of manuscripts. Thank you for always challenging me and improving my scientific thought processes. Many times, especially near the end you were as involved as a co-advisor and I truly appreciate all of the extra time you spent to help me through the final writing stages. Numerous members of the faculty and staff at Florida Gulf Coast University. To Drs. Darren Rumbold, Mike Parsons, Greg Tolley and Ai Ning Loh for countless hours discussing experimental design and methodologies, in addition to aid in editing of manuscripts and discussion of results and conclusions. I admire your focus on student advancement and your willingness to stop at a moment’s notice for an impromptu meeting and quick feedback and discussions. You have all greatly contributed to my development as a scientist and without your help and support this accomplishment would not have been possible. I wish you could be here to help celebrate the finale! Lesli Haynes, you really did keep my head from popping off on MANY occasions. I am so grateful for all of the help throughout this program, whether it was saving me with intern help at the last minute or just talking me off the ledge and back down to reality. It was always a re-motivating breath of fresh air to come down to the marine lab for a quick jam session. We shall most definitely raise a glass (or more) of Tito’s to celebrate this crazy journey! Bob Wasno, to many, many long days at the field station and countless field days; cold winter waters, getting thumped by territorial goliath grouper we couldn’t see, somehow managing to collect mussels with only 1” of visibility or none at all, sneak attacks from manatee, jelly fish stings…..the list goes on! And thank you for always knowing when it was Yingling time, which didn’t always mean it was time to stop working. I think we had some pretty brilliant ideas (not to mention building the red fish tank) over Yinglings in the Tuna Tower and pitchers at the Fish House. Thank you! David Segal, thank you SO much for being such a reliable and AMAZING science partner. I cannot tell you how amazing it was to get on the boat and know that you had the deck under control. You made my field days so easy knowing I had someone on board that I could trust to get the work done and make the right calls while I was underwater. And for somehow completely understanding what I was trying to explain while slightly insane and brain fried, probably not making any sense at all. And for moving at high speed when we had hundreds of samples to homogenize when the MixerMill finally arrived. You are the BEST! I truly could not have asked for a better lab mate to be by my side through this craziness! I am so glad we were able to work together, it was an absolute pleasure. To Romain Lavaud who guided me through the crazy world of DEB and who on many occasions kept my head from exploding. Romain without you I really would still be stuck at the DEB starting line. You have been an amazing support this past year, I don’t think I would have survived it without you! A huge thank you to students and staff of the Coastal Watershed Institute and Vester Marine Research Station for instrumental aid during field and laboratory work. Bob Wasno and David Segal for aid in the collection of mussels, even on holidays and weekends. And to Lesli Haynes, Lindsay Castret and David Segal for always being quick to help when I was in a bind and who were always there to keep me sane when things got crazy. To Christal Niemeyer and Tim Bryant for always keeping my orders and requests as a priority and squeezing me in when a last minute urgent order came up. To Norm Walker for always making sure I got paid and always being quick to get my user account fixed when I would get kicked off. To my friends and family who offered me endless support and motivation throughout this work. A special thanks to Anne and Julien Vignier, Dana Detmar, Ewan Harney for an amazing amount of support and encouragement. From acting as sounding boards and offering advice on a plethora of problems to just being there when the only solution was getting a cold beer. For my family who supported and encouraged me both emotionally and financially, I would not be here without your endless support and love. Thank you Mom and Dad! Most of all to Aaron Slusher who stood by me and cheered me through the good times and the bad. You were there to celebrate the little victories and help pick me back up after the defeats. Thank you for your patience and endless support and encouragement. I could not have made it through this last crazy last year without you to keep me sane and focused. Population dynamics of the invasive green mussel, Perna viridis, in southwest Florida and individual energetics through the application of the Dynamic Energy Budget Theory Table of Contents Abstract…………………………………………………………………………………..………..1 Resumé…………………………………………………………………………………...………..3 Abbreviations…………………………………………………………………………...…………6 Chapter1: General Introduction………………………………………………………………..8 History, origin and transport of green mussels ……….………………………….……….9 Study site: Estero Bay, Florida, USA………………………………………...………….11 Biology of P. viridis……………………………………………………………………..13 Environmental boundaries……………………………………………………………….21 Problems with invasive species: Biofouling and potential harm to oysters and ecosystem disruption………………………………………………………………………………...26 Understanding population dynamics through applying the Dynamic Energy Budget Theory to predict and prevent spread…………………………………………………….30 Chapter 2: Uptake and elimination of brevetoxin in the invasive green mussel, Perna viridis, during