CBX3 Antibody Cat. No.: 28-173 CBX3 Antibody Antibody used in WB on Human Jurkat cells at 1.25 ug/ml. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Dog, Human, Mouse, Rat Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human CBX3. TESTED APPLICATIONS: ELISA, IHC, WB CBX3 antibody can be used for detection of CBX3 by ELISA at 1:62500. CBX3 antibody can APPLICATIONS: be used for detection of CBX3 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. POSITIVE CONTROL: 1) Cat. No. 1205 - Jurkat Cell Lysate PREDICTED MOLECULAR 21 kDa, 21 kDa, 21 kDa WEIGHT: October 2, 2021 1 https://www.prosci-inc.com/cbx3-antibody-28-173.html Properties PURIFICATION: Antibody is purified by protein A chromatography method. CLONALITY: Polyclonal CONJUGATE: Unconjugated PHYSICAL STATE: Liquid Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose. CONCENTRATION: batch dependent For short periods of storage (days) store at 4˚C. For longer periods of storage, store CBX3 STORAGE CONDITIONS: antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. Additional Info OFFICIAL SYMBOL: CBX3 ALTERNATE NAMES: CBX3, HECH, HP1-GAMMA, HP1Hs-gamma ACCESSION NO.: NP_057671 PROTEIN GI NO.: 20544151 GENE ID: 11335 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. CBX3 binds DNA and is a component of heterochromatin. CBX3 also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of CBX3 may explain the association of heterochromatin with the inner nuclear membrane.At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the BACKGROUND: association of heterochromatin with the inner nuclear membrane. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene. October 2, 2021 2 https://www.prosci-inc.com/cbx3-antibody-28-173.html REFERENCES: 1) Scherer, S.W., (2003) Science 300 (5620), 767-772. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. October 2, 2021 3 https://www.prosci-inc.com/cbx3-antibody-28-173.html.