EVALUATION OF WOUND HEALING EFFECT OF AQUEOUS STEM EXTRACT OF Zanthoxylum rhetsa (Roxb.) DC. ON WISTAR RATS A Dissertation Submitted to THE TAMIL NADU Dr. M. G. R. MEDICAL UNIVERSITY CHENNAI-600 032 In partial fulfillment of the requirement for the award of the Degree of MASTER OF PHARMACY IN PHARMACOLOGY OCTOBER-2017 DEPARTMENT OF PHARMACOLOGY KMCH COLLEGE OF PHARMACY KOVAI ESTATE, KALAPPATTI ROAD, COIMBATORE-641048 EVALUATION OF WOUND HEALING EFFECT OF AQUEOUS STEM EXTRACT OF Zanthoxylum rhetsa (Roxb.) DC. ON WISTAR RATS A Dissertation submitted to THE TAMIL NADU Dr. M. G. R. MEDICAL UNIVERSITY CHENNAI-600 032 In partial fulfillment of the requirement for the award of the Degree of MASTER OF PHARMACY IN PHARMACOLOGY OCTOBER-2017 DEPARTMENT OF PHARMACOLOGY KMCH COLLEGE OF PHARMACY KOVAI ESTATE, KALAPPATTI ROAD, COIMBATORE-641 048 EVALUATION OF WOUND HEALING EFFECT OF AQUEOUS STEM EXTRACT OF Zanthoxylum rhetsa (Roxb.) DC. ON WISTAR RATS A Dissertation submitted to THE TAMIL NADU Dr. M .G .R. MEDICAL UNIVERSITY CHENNAI-600 032 In partial fulfillment of the requirement for the award of the Degree of MASTER OF PHARMACY IN PHARMACOLOGY OCTOBER-2017 Submitted by Reg. No. 261525809 DEPARTMENT OF PHARMACOLOGY KMCH COLLEGE OF PHARMACY KOVAI ESTATE, KALAPPATTI ROAD, COIMBATORE-641 048 Prof. Dr. A. Rajasekaran, M. Pharm., Ph.D., Principal, KMCH College of Pharmacy, Kovai Estate, Kalapatti Road, Coimbatore - 641 048. Tamil Nadu CERTIFICATE This is to certify that the dissertation work entitled “EVALUATION OF WOUND HEALING EFFECT OF AQUEOUS STEM EXTRACT OF Zanthoxylum rhetsa (Roxb.)DC. ON WISTAR RATS” was carried out by Reg. No. 261525809. The work mentioned in the dissertation was carried out at the Department of Pharmacology, KMCH College of Pharmacy, Coimbatore, Tamil Nadu, for the partial fulfillment for the degree of Master of Pharmacy during the academic year 2016-2017 and is forwarded to the Tamil Nadu Dr. M. G. R. Medical University, Chennai. Date: Prof. Dr. A. Rajasekaran, M. Pharm., Ph.D., Place: Coimbatore Principal GUIDE Dept. of Pharmacology, KMCH College of Pharmacy, Kovai Estate, Kalapatti Road, Coimbatore - 641 048. Tamil Nadu CERTIFICATE This is to certify that the dissertation work entitled “EVALUATION OF WOUND HEALING EFFECT OF AQUEOUS STEM EXTRACT OF Zanthoxylum rhetsa (Roxb.)DC. ON WISTAR RATS” is a bonafide work carried out by Reg. No. 261525809. The work mentioned in the dissertation was carried out at the Department of Pharmacology, KMCH College of Pharmacy, Coimbatore, Tamil Nadu, under my supervision and guidance during the academic year 2016-2017. This research work either in part or full does not constitute any of any thesis / dissertation. Date: Signature of the guide Place: Coimbatore DECLARATION I do here by declare that to the best of my knowledge and belief ,the dissertation work entitled “EVALUATION OF WOUND HEALING EFFECT OF AQUEOUS STEM EXTRACT OF Zanthoxylum rhetsa (Roxb.)DC. ON WISTAR RATS” submitted to the Tamil Nadu Dr. M.G.R. Medical university , Chennai, in the partial fulfillment for the Degree of Master of Pharmacy in Pharmacology, was carried out at Department of Pharmacology, KMCH College of Pharmacy, Coimbatore, during the academic year 2016-2017. Date: Place: Coimbatore Reg. No: 261525809 EVALUATION CERTIFICATE This is to certify that the work embodied in the thesis entitled “EVALUATION OF WOUND HEALING EFFECT OF AQUEOUS STEM EXTRACT OF Zanthoxylum rhetsa (Roxb.)DC. ON WISTAR RATS” submitted by Reg No: 261525809 to the Tamil Nadu Dr. M.G.R. Medical university, Chennai, in the partial fulfillment for the Degree of Master of Pharmacy in Pharmacology, is a bonafide research work carried out by the candidate during the academic year 2016- 2017 at KMCH College of Pharmacy, Coimbatore, Tamil Nadu and the same was evaluated by us. Examination Center: KMCH College of Pharmacy, Coimbatore Date: Place: Coimbatore Internal Examiner External Examiner Convener of Examination EVALUATION OF WOUND HEALING EFFECT OF AQUEOUS STEM EXTRACT OF Zanthoxylum rhetsa (Roxb.) DC. ON WISTAR RATS A Dissertation submitted to THE TAMIL NADU Dr. M. G. R. MEDICAL UNIVERSITY CHENNAI-600 032 In partial fulfillment of the requirement for the award of the Degree of MASTER OF PHARMACY IN PHARMACOLOGY OCTOBER-2017 DEPARTMENT OF PHARMACOLOGY KMCH COLLEGE OF PHARMACY KOVAI ESTATE, KALAPPATTI ROAD, COIMBATORE-641 048 EVALUATION OF WOUND HEALING EFFECT OF AQUEOUS STEM EXTRACT OF Zanthoxylum rhetsa (Roxb.) DC. ON WISTAR RATS A Dissertation submitted to THE TAMIL NADU Dr. M. G. R. MEDICAL UNIVERSITY CHENNAI-600 032 In partial fulfillment of the requirement for the award of the Degree of MASTER OF PHARMACY IN PHARMACOLOGY OCTOBER-2017 Submitted by S. Parthiban (Reg. No. 261525809) Under the Guidance of Mrs. A. Abarnadevika, M.Pharm., Asst. Professor, Department of Pharmacology DEPARTMENT OF PHARMACOLOGY KMCH COLLEGE OF PHARMACY, KOVAI ESTATE, KALAPATTI ROAD, COIMBATORE-641 048. Prof. Dr. A. Rajasekaran, M. Pharm., Ph.D., Principal, KMCH College of Pharmacy, Kovai Estate, Kalapatti Road, Coimbatore - 641 048. Tamil Nadu CERTIFICATE This is to certify that the dissertation work entitled “EVALUATION OF WOUND HEALING EFFECT OF AQUEOUS STEM EXTRACT OF Zanthoxylum rhetsa (Roxb.) DC. ON WISTAR RATS” was carried out by S. Parthiban (Reg. No. 261525809). The work mentioned in the dissertation was carried out at the Department of Pharmacology, KMCH College of Pharmacy, Coimbatore, Tamil Nadu, under the guidance of Mrs. A. Abarnadevika, M. Pharm., for the partial fulfillment for the degree of Master of Pharmacy during the academic year 2016-2017 and is forwarded to the Tamil Nadu Dr. M. G. R. Medical University, Chennai. Date: Prof. Dr. A. Rajasekaran, M. Pharm., Ph.D., Place: Coimbatore Principal Mrs. A. Abarnadevika, M. Pharm., Asst. Professor, Dept. of Pharmacology, KMCH College of Pharmacy, Kovai Estate, Kalapatti Road, Coimbatore - 641 048. Tamil Nadu CERTIFICATE This is to certify that the dissertation work entitled “EVALUATION OF WOUND HEALING EFFECT OF AQUEOUS STEM EXTRACT OF Zanthoxylum rhetsa (Roxb.) DC. ON WISTAR RATS” is a bonafide work carried out by S. Parthiban (Reg. No. 261525809). The work mentioned in the dissertation was carried out at the Department of Pharmacology, KMCH College of Pharmacy, Coimbatore, Tamil Nadu, under my supervision and guidance during the academic year 2016-2017. This research work either in part or full does not constitute any of any thesis / dissertation. Date: Mrs. A. Abarnadevika, M. Pharm., DECLARATION I do here by declare that to the best of my knowledge and belief ,the dissertation work entitled “EVALUATION OF WOUND HEALING EFFECT OF AQUEOUS STEM EXTRACT OF Zanthoxylum rhetsa (Roxb.)DC. ON WISTAR RATS” submitted to the Tamil Nadu Dr. M. G. R. Medical university , Chennai, in the partial fulfillment for the Degree of Master of Pharmacy in Pharmacology, was carried out at Department of Pharmacology, KMCH College of Pharmacy, Coimbatore under the guidance of Mrs. A. Abarnadevika, M. Pharm., during the academic year 2016-2017. Date: S. Parthiban Place: Coimbatore Reg No: (261525809) EVALUATION CERTIFICATE This is to certify that the work embodied in the thesis entitled “EVALUATION OF WOUND HEALING EFFECT OF AQUEOUS STEM EXTRACT OF Zanthoxylum rhetsa (Roxb.)DC. ON WISTAR RATS” submitted by S. Parthiban to the Tamil Nadu Dr. M.G.R. Medical university, Chennai, in the partial fulfillment for the Degree of Master of Pharmacy in Pharmacology, is a bonafide research work carried out by the candidate during the academic year 2016-2017 at KMCH College of Pharmacy, Coimbatore, Tamil Nadu and the same was evaluated by us. Examination Center: KMCH College of Pharmacy, Coimbatore Date: Place: Coimbatore Internal Examiner External Examiner Convener of Examination ACKNOWLEDGEMENT By the blessings of Lord, encouragement of parents, continuous guidance and support of my esteemed teachers and timely help of my friends and peers, who orchestrated this work. I take an opportunity to thank them for making me to achieve the desired goal. Many thanks to Almighty God, for it He who began this work in me and carried it to completion. It is he who has blessed me with the people whose names I feel privileged to mention here. I would like to express my whole hearted gratitude to my father, mother and grandmother, without whose blessings, love, and inspiration, this endeavor would not have been completed. I take this opportunity to express my sincere gratitude and indebtedness and heartfelt thanks to my esteemed research guide, Mrs. A. Abarnadevika, M.Pharm, Asst.Professor, Department of Pharmacology, who by his constant evaluation made sure that I stayed focused on my work and with his continued encouragement motivated me to learn more. I would like to thank Dr. K. T. Manisenthil Kumar, HOD and Professor, Department of Pharmacology, for suggesting and guiding me throughout. I will forever be grateful for his invaluable ideas and support. His patience has always encouraged me in learning many things in this work and in my life too. It is my privilege to thank Dr. A. Rajasekaran, Principal, KMCH College of Pharmacy, Coimbatore, who has provided excellent facilities to do research in this institution. I proffer my sincere thanks and show beholden to our beloved Managing Trustee, Dr. Nalla G. Palaniswami and respected Trustee madam Dr. Thavamani D. Palaniswami, Kovai Medical Center Research and Educational trust, Coimbatore for all the facilities that were provided to me at the institution. I express my deep gratitude to Dr. G. Ariharasivakumar, M.pharm.,PhD. Professor, Dr. K.S.G. Arulkumaran M.Pharm., PhD., Dr. N. Adhirajan, M.Pharm.,Ph.D., Dr. C. Sundaramoorthy, M.Pharm Ph.D., Dr. Arivukkarasu, M.Pharm., Ph.D., Dr. M Senthil Kumar, M.Pharm.,PhD., Mr. Ramasamy, Mr. J. Saravanan, Mrs.J.Vennila for their sensible help and suggestions. It was a pleasure to share Master studies with wonderful people. I am greatly indebted to all my loving friends T.Boopathi, Anusree, Anu Sebastain, Anna Mathew, Neethu Dhevasia.